1N4A
The Ligand Bound Structure of E.coli BtuF, the Periplasmic Binding Protein for Vitamin B12
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0005515 | molecular_function | protein binding |
| A | 0015889 | biological_process | cobalamin transport |
| A | 0016020 | cellular_component | membrane |
| A | 0030288 | cellular_component | outer membrane-bounded periplasmic space |
| A | 0031419 | molecular_function | cobalamin binding |
| A | 0042597 | cellular_component | periplasmic space |
| A | 1990191 | cellular_component | cobalamin transport complex |
| B | 0005515 | molecular_function | protein binding |
| B | 0015889 | biological_process | cobalamin transport |
| B | 0016020 | cellular_component | membrane |
| B | 0030288 | cellular_component | outer membrane-bounded periplasmic space |
| B | 0031419 | molecular_function | cobalamin binding |
| B | 0042597 | cellular_component | periplasmic space |
| B | 1990191 | cellular_component | cobalamin transport complex |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 33 |
| Details | BINDING SITE FOR RESIDUE CNC A 501 |
| Chain | Residue |
| A | PRO9 |
| A | SER219 |
| A | ASP220 |
| A | GLU223 |
| A | ARG224 |
| A | HOH7034 |
| A | HOH7035 |
| A | HOH7046 |
| A | HOH7048 |
| A | HOH7069 |
| A | HOH7115 |
| A | ALA10 |
| A | HOH7143 |
| A | HOH7167 |
| A | HOH7179 |
| A | HOH7187 |
| A | HOH7203 |
| A | HOH7225 |
| A | HOH7250 |
| A | HOH7325 |
| A | HOH7376 |
| A | HOH7431 |
| A | TYR28 |
| A | HOH7436 |
| A | HOH7497 |
| A | HOH7503 |
| A | HOH7540 |
| A | TRP44 |
| A | TRP63 |
| A | GLY65 |
| A | GLY66 |
| A | PHE146 |
| A | TRP174 |
| site_id | AC2 |
| Number of Residues | 19 |
| Details | BINDING SITE FOR RESIDUE CNC B 5501 |
| Chain | Residue |
| B | PRO5009 |
| B | ALA5010 |
| B | SER5027 |
| B | TYR5028 |
| B | TRP5044 |
| B | TRP5063 |
| B | GLY5065 |
| B | GLY5066 |
| B | TRP5174 |
| B | SER5219 |
| B | ASP5220 |
| B | GLU5223 |
| B | ARG5224 |
| B | HOH7010 |
| B | HOH7083 |
| B | HOH7109 |
| B | HOH7141 |
| B | HOH7475 |
| B | HOH7520 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_01000, ECO:0000269|PubMed:12468528, ECO:0007744|PDB:1N4A |
| Chain | Residue | Details |
| A | TYR28 | |
| B | TYR5028 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_01000, ECO:0000269|PubMed:12468528, ECO:0000269|PubMed:12475936, ECO:0007744|PDB:1N2Z, ECO:0007744|PDB:1N4A |
| Chain | Residue | Details |
| A | ASP220 | |
| B | ASP5220 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 4 |
| Details | SITE: Important for BtuC binding => ECO:0000255|HAMAP-Rule:MF_01000, ECO:0000305|PubMed:12475936 |
| Chain | Residue | Details |
| A | GLU50 | |
| A | GLU180 | |
| B | GLU5050 | |
| B | GLU5180 |
