1LWK

Multiple Methionine Substitutions are Tolerated in T4 Lysozyme and have Coupled Effects on Folding and Stability

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Lysozyme	polymer	164	19541.4	1	UniProt (P00720) Pfam (PF00959) In PDB	Enterobacteria phage T4	Lysis protein, Muramidase, Endolysin
2	A	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
3	A	2-HYDROXYETHYL DISULFIDE	non-polymer		154.3	1	Chemie (HED)
4		water	water		18.0	77	Chemie (HOH)

Sequence modifications

A: 1 - 164 (UniProt: P00720)

PDB	External Database	Details
Mse 1	Met 1	modified residue
Mse 6	Met 6	modified residue
Thr 54	Cys 54	engineered mutation
Mse 84	Leu 84	engineered mutation
Mse 87	Val 87	engineered mutation
Mse 91	Leu 91	engineered mutation
Ala 97	Cys 97	engineered mutation
Mse 99	Leu 99	engineered mutation
Mse 102	Met 102	modified residue
Mse 106	Met 106	modified residue
Arg 110	Gly 110	engineered mutation
Mse 111	Val 111	engineered mutation
Mse 118	Leu 118	engineered mutation
Mse 120	Met 120	modified residue
Mse 121	Leu 121	engineered mutation
Mse 133	Leu 133	engineered mutation
Mse 153	Phe 153	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	19541.4
Non-Polymers*	Number of molecules	3
	Total formula weight	225.2
All*	Total formula weight	19766.6

*Water molecules are not included.