1LWK
Multiple Methionine Substitutions are Tolerated in T4 Lysozyme and have Coupled Effects on Folding and Stability
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 173 |
Detector technology | IMAGE PLATE |
Collection date | 2002-02-10 |
Detector | MARRESEARCH |
Wavelength(s) | 0.82653 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 60.332, 60.332, 91.367 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 60.000 - 2.100 |
R-factor | 0.219 |
Rwork | 0.217 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1l63 |
RMSD bond length | 0.018 |
RMSD bond angle | 2.900 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | TNT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 11279 | |
Completeness [%] | 99.2 | 99.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | VAPOR DIFFUSION, HANGING DROP |