1KL3
an engineered streptavidin with improved affinity for the strep-tag II peptide : SAm1-StrepII
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | streptavidin | polymer | 127 | 13383.6 | 4 | UniProt (P22629) Pfam (PF01382) | Streptomyces avidinii | |
| 2 | E, F, G, H (E, F, G, H) | strep-tag II peptide | polymer | 9 | 1174.3 | 4 | |||
| 3 | I, J, K, L, M... (A, B, C, D, E...) | water | water | 18.0 | 238 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 14 - 139 (UniProt: P22629)
| PDB | External Database | Details |
|---|---|---|
| Met 13 | - | initiating methionine |
| Val 44 | Glu 68 | engineered mutation |
| Thr 45 | Ser 69 | engineered mutation |
| Arg 47 | Val 71 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 58231.3 | |
| All* | Total formula weight | 58231.3 |
