1KL3

an engineered streptavidin with improved affinity for the strep-tag II peptide : SAm1-StrepII

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	2001-07-21
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	58.699, 86.339, 47.448
Unit cell angles	90.00, 98.92, 90.00

Refinement procedure

Resolution	36.000 - 1.700
R-factor	0.175 *
Rwork	0.175
R-free	0.21700 *
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1swu
RMSD bond length	0.018
RMSD bond angle	2.773
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000 *	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.040 *
Total number of observations	126949 *
Number of reflections	49002
<I/σ(I)>	23.8	3.2
Completeness [%]	94.8	90.3
Redundancy	2.6	2.38

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	20 *	100 mM Na2HPO4, 1.3-M (NH4)2SO4, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	22 (mg/ml)
2	1	drop	Tris-HCl	5 (mM)	pH8.0