1KIE

Inosine-adenosine-guanosine preferring nucleoside hydrolase from Trypanosoma vivax: Asp10Ala mutant in complex with 3-deaza-adenosine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	inosine-adenosine-guanosine preferring nucleoside hydrolase	polymer	339	37675.0	2	UniProt (Q9GPQ4) Pfam (PF01156) In PDB	Trypanosoma vivax	IAG-NUCLEOSIDE HYDROLASE, IAG-NH
2	A, B	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
3	A	NICKEL (II) ION	non-polymer		58.7	1	Chemie (NI)
4	A, B	3-DEAZA-ADENOSINE	non-polymer		266.3	2	Chemie (AD3)
5		water	water		18.0	604	Chemie (HOH)

Sequence modifications

A, B: 2 - 327 (UniProt: Q9GPQ4)

PDB	External Database	Details
Met -12	-	expression tag
Arg -11	-	expression tag
Gly -10	-	expression tag
Ser -9	-	expression tag
Pro -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Ala 10	Asp 10	engineered mutation
Asn 301	Lys 301	SEE REMARK 999

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	75349.9
Non-Polymers*	Number of molecules	5
	Total formula weight	671.4
All*	Total formula weight	76021.3

*Water molecules are not included.