1K6N

E(L212)A,D(L213)A Double Mutant Structure of Photosynthetic Reaction Center from Rhodobacter Sphaeroides

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (L)	PHOTOSYNTHETIC REACTION CENTER L SUBUNIT	polymer	281	31244.3	1	UniProt (P02954) Pfam (PF00124) UniProt (by SIFTS) (P0C0Y8)	Rhodobacter sphaeroides	Reaction center protein L chain
2	B (M)	PHOTOSYNTHETIC REACTION CENTER M SUBUNIT	polymer	314	35365.6	1	UniProt (P02953) Pfam (PF00124) UniProt (by SIFTS) (P0C0Y9)	Rhodobacter sphaeroides	Reaction center protein M chain
3	C (H)	PHOTOSYNTHETIC REACTION CENTER H SUBUNIT	polymer	260	28066.3	1	UniProt (P11846) Pfam (PF03967) Pfam (PF05239) UniProt (by SIFTS) (P0C0Y7)	Rhodobacter sphaeroides	Reaction center protein H chain
4	D, E, J, K (L, M)	BACTERIOCHLOROPHYLL A	non-polymer		911.5	4	Chemie (BCL)
5	F, L (L, M)	BACTERIOPHEOPHYTIN A	non-polymer		889.2	2	Chemie (BPH)
6	G, M (L, M)	UBIQUINONE-10	non-polymer		863.3	2	Chemie (U10)
7	H, P, Q, R (L, M, H)	LAURYL DIMETHYLAMINE-N-OXIDE	non-polymer		229.4	4	Chemie (LDA)
8	I (M)	FE (III) ION	non-polymer		55.8	1	Chemie (FE)
9	N (M)	SPEROIDENONE	non-polymer		595.0	1	Chemie (SPN)
10	O (M)	CARDIOLIPIN	non-polymer		1464.0	1	Chemie (CDL)
11	S, T, U (L, M, H)	water	water		18.0	129	Chemie (HOH)

Sequence modifications

L: 1 - 281 (UniProt: P02954)

PDB	External Database	Details
Ala 212	Glu 212	engineered mutation
Ala 213	Asp 213	engineered mutation

M: 1 - 307 (UniProt: P02953)

PDB	External Database	Details
His 308	-	expression tag
His 309	-	expression tag
His 310	-	expression tag
His 311	-	expression tag
His 312	-	expression tag
His 313	-	expression tag
His 314	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	94676.2
Non-Polymers*	Number of molecules	15
	Total formula weight	10183.6
All*	Total formula weight	104859.8

*Water molecules are not included.