1K6N

E(L212)A,D(L213)A Double Mutant Structure of Photosynthetic Reaction Center from Rhodobacter Sphaeroides

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	2000-01-06
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.54
Spacegroup name	P 31 2 1
Unit cell lengths	141.500, 141.500, 187.400
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 3.100
Rwork	0.203
R-free	0.20740
Structure solution method	rigid-body refinement
Starting model (for MR)	1k6l
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000 *	3.210
High resolution limit [Å]	3.100	3.100
Rmerge	0.099	0.246
Number of reflections	31830 *
<I/σ(I)>	12.5	3.6
Completeness [%]	92.0 *	85 *
Redundancy	2.3	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	potassium phosphate, ldao, heptane triol, dioxane, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K