1H6Y

The role of conserved amino acids in the cleft of the C-terminal family 22 carbohydrate binding module of Clostridium thermocellum Xyn10B in ligand binding

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	ENDO-1,4-BETA-XYLANASE Y	polymer	170	18939.8	2	UniProt (P51584) Pfam (PF02018)	CLOSTRIDIUM THERMOCELLUM	CBM22-2,XYLANASE Y, XYLY, 1,4-BETA-D-XYLAN XYLANOHYDROLASE Y
2	C, D (A, B)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
3	E, F (A, B)	water	water		18.0	296	Chemie (HOH)

Sequence modifications

A, B: 2 - 162 (UniProt: P51584)

PDB	External Database	Details
Met 1	-	expression tag
Ala 138	Glu 696	engineered mutation
Leu 163	-	expression tag
Glu 164	-	expression tag
His 165	-	expression tag
His 166	-	expression tag
His 167	-	expression tag
His 168	-	expression tag
His 169	-	expression tag
His 170	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	37879.7
Non-Polymers*	Number of molecules	2
	Total formula weight	80.2
All*	Total formula weight	37959.9

*Water molecules are not included.