1H6Y
The role of conserved amino acids in the cleft of the C-terminal family 22 carbohydrate binding module of Clostridium thermocellum Xyn10B in ligand binding
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-07-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 89.869, 89.869, 209.003 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 2.200 |
| Rwork | 0.194 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dyo |
| RMSD bond length | 0.011 * |
| RMSD bond angle | 1.377 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.068 | 0.350 |
| Number of reflections | 26510 | |
| <I/σ(I)> | 24 | 5.8 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 28.3 | 25.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.6 | 0.1 M NAAC(PH 4.6),10 MM DTT, 25% GLYCEROL,12% PEG8000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 50 (mg/ml) | |
| 2 | 1 | drop | 0.1 (mg/ml) | pH4.6 | |
| 3 | 1 | drop | dithiothreitol | 10 (mM) | |
| 4 | 1 | drop | glycerol | 25 (%(v/v)) | |
| 5 | 1 | drop | PEG8000 | 12 (%(w/v)) |
