1GNV

CALCIUM INDEPENDENT SUBTILISIN BPN' MUTANT

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	SUBTILISIN BPN'	polymer	266	26771.6	1	UniProt (P00782) Pfam (PF00082)	BACILLUS AMYLOLIQUEFACIENS	ALKALINE PROTEASE
2	B (A)	water	water		18.0	133	Chemie (HOH)

Sequence modifications

A: 1 - 74 (UniProt: P00782)

PDB	External Database	Details
Lys 2	Glu 103	engineered mutation
Cys 3	Ser 104	engineered mutation
Ser 5	Pro 106	engineered mutation
Asn 43	Lys 144	engineered mutation
Phe 50	Met 151	engineered mutation
Leu 73	Ala 174	engineered mutation

A: 84 - 275 (UniProt: P00782)

PDB	External Database	Details
Cys 206	Glu 307	engineered mutation
Lys 217	Tyr 318	engineered mutation
Ser 218	Asn 319	engineered mutation
Mis 221	Ser 322	engineered mutation
Glu 271	Gln 372	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	26771.6
All*	Total formula weight	26771.6

*Water molecules are not included.