1GNV

CALCIUM INDEPENDENT SUBTILISIN BPN' MUTANT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	AREA DETECTOR
Collection date	1994-07-15
Detector	SIEMENS MULTIWIRE
Spacegroup name	P 21 21 21
Unit cell lengths	54.200, 60.400, 82.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.900
Rwork	0.176
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1sua
RMSD bond length	0.020
RMSD bond angle	0.040
Data reduction software	XENGEN
Data scaling software	XENGEN
Phasing software	AMoRE
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	8.000
High resolution limit [Å]	1.900
Rmerge	0.088	0.088
Total number of observations	72438 *
Number of reflections	17067 *
<I/σ(I)>	6
Completeness [%]	86.0
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	9 *	20 *	23% PEG 4K, O.2M AMMONIUM SULFATE, pH 7.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	glycine	0.05 (M)	pH9.0
3	1	reservoir	acetone	55 (%)
4	1	reservoir	glycine	0.05 (M)