1GNS

SUBTILISIN BPN'

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	SUBTILISIN BPN'	polymer	263	26400.3	1	UniProt (P00782) Pfam (PF00082) In PDB	BACILLUS AMYLOLIQUEFACIENS
2	A	ACETONE	non-polymer		58.1	1	Chemie (ACN)
3		water	water		18.0	155	Chemie (HOH)

Sequence modifications

A: 4 - 74 (UniProt: P00782)

PDB	External Database	Details
Gly 4	Val 111	conflict
Ala 41	Asp 148	engineered mutation
Phe 50	Met 157	engineered mutation
Leu 73	Ala 180	engineered mutation

A: 84 - 275 (UniProt: P00782)

PDB	External Database	Details
Trp 206	Gln 313	engineered mutation
Lys 217	Tyr 324	engineered mutation
Ser 218	Asn 325	engineered mutation
Cso 221	Ser 328	engineered mutation
Glu 271	Gln 378	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	26400.3
Non-Polymers*	Number of molecules	1
	Total formula weight	58.1
All*	Total formula weight	26458.4

*Water molecules are not included.