1GNS
SUBTILISIN BPN'
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Collection date | 1994-07-15 |
| Detector | SIEMENS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.170, 78.060, 36.700 |
| Unit cell angles | 90.00, 114.60, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.800 |
| R-factor | 0.165 |
| Rwork | 0.165 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1suc |
| RMSD bond length | 0.020 |
| RMSD bond angle | 0.040 |
| Data reduction software | XENGEN |
| Data scaling software | XENGEN |
| Phasing software | AMoRE |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 8.000 |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.055 |
| Total number of observations | 64819 * |
| Number of reflections | 15590 |
| Completeness [%] | 77.0 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 9 * | 20 * | 55% ACETONE, 0.05M GLYCINE PH 9.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | glycine | 0.05 (M) | pH9.0 |
| 3 | 1 | reservoir | acetone | 55 (%) | |
| 4 | 1 | reservoir | glycine | 0.05 (M) |
