1GNS
SUBTILISIN BPN'
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 293 |
Detector technology | AREA DETECTOR |
Collection date | 1994-07-15 |
Detector | SIEMENS |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 41.170, 78.060, 36.700 |
Unit cell angles | 90.00, 114.60, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.800 |
R-factor | 0.165 |
Rwork | 0.165 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1suc |
RMSD bond length | 0.020 |
RMSD bond angle | 0.040 |
Data reduction software | XENGEN |
Data scaling software | XENGEN |
Phasing software | AMoRE |
Refinement software | PROLSQ |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 8.000 |
High resolution limit [Å] | 1.800 |
Rmerge | 0.055 |
Total number of observations | 64819 * |
Number of reflections | 15590 |
Completeness [%] | 77.0 |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 9 * | 20 * | 55% ACETONE, 0.05M GLYCINE PH 9.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | glycine | 0.05 (M) | pH9.0 |
3 | 1 | reservoir | acetone | 55 (%) | |
4 | 1 | reservoir | glycine | 0.05 (M) |