1FI8

RAT GRANZYME B [N66Q] COMPLEXED TO ECOTIN [81-84 IEPD]

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	NATURAL KILLER CELL PROTEASE 1	polymer	228	25225.4	2	UniProt (P18291) Pfam (PF00089) In PDB	Rattus norvegicus (Norway rat)	GRANZYME B
2	C, E	ECOTIN	polymer	84	9528.7	2	UniProt (P23827) Pfam (PF03974) In PDB	Escherichia coli
3	D, F	ECOTIN	polymer	58	6645.7	2	UniProt (P23827) Pfam (PF03974) In PDB	Escherichia coli
4		water	water		18.0	370	Chemie (HOH)

Sequence modifications

A, B: 16 - 245 (UniProt: P18291)

PDB	External Database	Details
Gln 66	Asn 72	engineered mutation

C, E: 6 - 84 (UniProt: P23827)

PDB	External Database	Details
Ile 81	Val 108	engineered mutation
Glu 82	Ser 109	engineered mutation
Pro 83	Thr 110	engineered mutation
Asp 84	Met 111	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	82799.8
All*	Total formula weight	82799.8

*Water molecules are not included.