1F4D

CRYSTAL STRUCTURE OF E. COLI THYMIDYLATE SYNTHASE C146S, L143C COVALENTLY MODIFIED AT C143 WITH N-[TOSYL-D-PROLINYL]AMINO-ETHANETHIOL

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	THYMIDYLATE SYNTHASE	polymer	264	30533.6	2	UniProt (P0A884) Pfam (PF00303) In PDB	Escherichia coli
2	A, B	SULFATE ION	non-polymer		96.1	4	Chemie (SO4)
3	A, B	N-[TOSYL-D-PROLINYL]AMINO-ETHANETHIOL	non-polymer		328.4	2	Chemie (TP2)
4	A, B	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
5		water	water		18.0	297	Chemie (HOH)

Sequence modifications

A, B: 1 - 264 (UniProt: P0A884)

PDB	External Database	Details
Cxm 1	Met 1	engineered mutation
Cys 143	Leu 143	engineered mutation
Ser 146	Cys 146	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	61067.2
Non-Polymers*	Number of molecules	8
	Total formula weight	1225.3
All*	Total formula weight	62292.5

*Water molecules are not included.