1EB8

Structure Determinants of Substrate Specificity of Hydroxynitrile Lyase from Manihot esculenta

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	(S)-ACETONE-CYANOHYDRIN LYASE	polymer	262	29720.1	2	UniProt (P52705) Pfam (PF00561) In PDB	MANIHOT ESCULENTA (CASSAVA)	(S)-HYDROXYNITRILE LYASE, (S)-HYDROXYNITRILASE, OXYNITRILASE, HYDROXYNITRILE LYASE
2	A, B	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	2	Chemie (MPD)
3		water	water		18.0	250	Chemie (HOH)

Sequence modifications

A, B: -4 - 1 (PDB: 1EB8)
A, B: 2 - 258 (UniProt: P52705)

PDB	External Database	Details
Ala 128	Trp 127	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	59440.2
Non-Polymers*	Number of molecules	2
	Total formula weight	236.3
All*	Total formula weight	59676.5

*Water molecules are not included.