1EB8
Structure Determinants of Substrate Specificity of Hydroxynitrile Lyase from Manihot esculenta
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0009694 | biological_process | jasmonic acid metabolic process |
| A | 0009696 | biological_process | salicylic acid metabolic process |
| A | 0016829 | molecular_function | lyase activity |
| A | 0047606 | molecular_function | hydroxynitrilase activity |
| A | 0052891 | molecular_function | aliphatic (S)-hydroxynitrile lyase activity |
| A | 0052892 | molecular_function | aromatic (S)-hydroxynitrile lyase activity |
| A | 0080030 | molecular_function | methyl indole-3-acetate esterase activity |
| A | 0080031 | molecular_function | methyl salicylate esterase activity |
| A | 0080032 | molecular_function | methyl jasmonate esterase activity |
| B | 0009694 | biological_process | jasmonic acid metabolic process |
| B | 0009696 | biological_process | salicylic acid metabolic process |
| B | 0016829 | molecular_function | lyase activity |
| B | 0047606 | molecular_function | hydroxynitrilase activity |
| B | 0052891 | molecular_function | aliphatic (S)-hydroxynitrile lyase activity |
| B | 0052892 | molecular_function | aromatic (S)-hydroxynitrile lyase activity |
| B | 0080030 | molecular_function | methyl indole-3-acetate esterase activity |
| B | 0080031 | molecular_function | methyl salicylate esterase activity |
| B | 0080032 | molecular_function | methyl jasmonate esterase activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE MPD A1301 |
| Chain | Residue |
| A | SER80 |
| A | HOH2041 |
| site_id | AC2 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE MPD B1301 |
| Chain | Residue |
| B | ILE12 |
| B | HOH2058 |
| B | HOH2134 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | ACT_SITE: Proton donor/acceptor => ECO:0000305|PubMed:11173464, ECO:0000305|PubMed:11316882 |
| Chain | Residue | Details |
| A | CYS81 | |
| A | LYS237 | |
| B | CYS81 | |
| B | LYS237 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 6 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:11173464, ECO:0007744|PDB:1DWO |
| Chain | Residue | Details |
| A | ILE12 | |
| A | CYS81 | |
| A | ALA82 | |
| B | ILE12 | |
| B | CYS81 | |
| B | ALA82 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | SITE: Increases basicity of active site His => ECO:0000305|PubMed:11316882 |
| Chain | Residue | Details |
| A | LYS209 | |
| B | LYS209 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1c4x |
| Chain | Residue | Details |
| A | SER80 | |
| A | HIS236 | |
| A | ASP208 | |
| A | THR11 |
| site_id | CSA2 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1c4x |
| Chain | Residue | Details |
| B | SER80 | |
| B | HIS236 | |
| B | ASP208 | |
| B | THR11 |
| site_id | CSA3 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1c4x |
| Chain | Residue | Details |
| A | SER80 | |
| A | HIS236 | |
| A | ASP208 |
| site_id | CSA4 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1c4x |
| Chain | Residue | Details |
| B | SER80 | |
| B | HIS236 | |
| B | ASP208 |
