1CGV
SITE DIRECTED MUTATIONS OF THE ACTIVE SITE RESIDUE TYROSINE 195 OF CYCLODEXTRIN GLYCOSYLTRANSFERASE FROM BACILLUS CIRCULANS STRAIN 251 AFFECTING ACTIVITY AND PRODUCT SPECIFICITY
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | CYCLOMALTODEXTRIN GLUCANOTRANSFERASE | polymer | 686 | 74559.5 | 1 | UniProt (P43379) Pfam (PF00128) Pfam (PF02806) Pfam (PF01833) Pfam (PF00686) | Bacillus circulans | |
| 2 | B, C, D (B, C, D) | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 342.3 | 3 | In PDB BIRD (PRD_900001) GlyTouCan (G07411ON) | alpha-maltose | ||
| 3 | E, F (A) | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
| 4 | G (A) | water | water | 18.0 | 171 | Chemie (HOH) |
Sequence modifications
A: 1 - 686 (UniProt: P43379)
| PDB | External Database | Details |
|---|---|---|
| Phe 195 | Tyr 222 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 74559.5 | |
| Branched | Number of molecules | 3 |
| Total formula weight | 1026.9 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 80.2 | |
| All* | Total formula weight | 75666.5 |
