1CGV

SITE DIRECTED MUTATIONS OF THE ACTIVE SITE RESIDUE TYROSINE 195 OF CYCLODEXTRIN GLYCOSYLTRANSFERASE FROM BACILLUS CIRCULANS STRAIN 251 AFFECTING ACTIVITY AND PRODUCT SPECIFICITY

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	121.031, 111.107, 66.302
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.500
R-factor	0.155
RMSD bond length	0.010 *
RMSD bond angle	2.100 *
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.000 *
High resolution limit [Å]	2.500 *	2.500 *
Rmerge	0.049 *
Total number of observations	84161 *
Number of reflections	28296 *
Completeness [%]	87.3 *	45 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *