1C0G

CRYSTAL STRUCTURE OF 1:1 COMPLEX BETWEEN GELSOLIN SEGMENT 1 AND A DICTYOSTELIUM/TETRAHYMENA CHIMERA ACTIN (MUTANT 228: Q228K/T229A/A230Y/E360H)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	S	PROTEIN (GELSOLIN SEGMENT 1)	polymer	127	14205.0	1	UniProt (P06396) Pfam (PF00626) In PDB	Homo sapiens (human)
2	A	PROTEIN (CHIMERIC ACTIN)	polymer	375	41732.6	1	UniProt (P07830) Pfam (PF00022) In PDB	Dictyostelium discoideum Tetrahymena thermophila ( )
3	S, A	CALCIUM ION	non-polymer		40.1	3	Chemie (CA)
4	A	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	1	Chemie (ATP)
5		water	water		18.0	469	Chemie (HOH)

Sequence modifications

S: 4 - 127 (UniProt: P06396)

PDB	External Database	Details
Met 1	-	cloning artifact
Gly 2	-	cloning artifact
Ser 3	-	cloning artifact

A: 1 - 375 (UniProt: P07830)

PDB	External Database	Details
Hic 73	His 74	modified residue
Ala 225	Gln 226	conflict
Lys 228	Ala 229	SEE REMARK 999
Ala 229	Thr 230	SEE REMARK 999
Tyr 230	Ala 231	SEE REMARK 999
His 360	Glu 361	SEE REMARK 999

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	55937.6
Non-Polymers*	Number of molecules	4
	Total formula weight	627.4
All*	Total formula weight	56565.0

*Water molecules are not included.