1C0G
CRYSTAL STRUCTURE OF 1:1 COMPLEX BETWEEN GELSOLIN SEGMENT 1 AND A DICTYOSTELIUM/TETRAHYMENA CHIMERA ACTIN (MUTANT 228: Q228K/T229A/A230Y/E360H)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-06-23 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 0.708 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.862, 69.030, 181.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.000 |
Rwork | 0.182 |
R-free | 0.22300 |
RMSD bond length | 0.011 * |
RMSD bond angle | 1.900 * |
Data scaling software | CCP4 ((SCALA)) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.064 | 0.149 |
Total number of observations | 161420 * | |
Number of reflections | 45284 | |
<I/σ(I)> | 8 | |
Completeness [%] | 92.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 295 | MES, SODIUM CHLORIDE, ATP, CALCIUM CHLORIDE, MAGNESIUM CHLORIDE, SODIUM AZIDE, DITHIOTHREITOL, PEG 6000, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
10 | 1 | reservoir | 150 (mM) | ||
11 | 1 | reservoir | ATP | 0.1 (mM) | |
12 | 1 | reservoir | 0.1 (mM) | ||
13 | 1 | reservoir | 0.1 (mM) | ||
14 | 1 | reservoir | 1 (mM) | ||
15 | 1 | reservoir | dithiothreitol | 10 (mM) | |
2 | 1 | drop | imidazole-HCl | 10 (mM) | |
3 | 1 | drop | 150 (mM) | ||
4 | 1 | drop | ATP | 0.1 (mM) | |
5 | 1 | drop | 0.1 (mM) | ||
6 | 1 | drop | 0.1 (mM) | ||
7 | 1 | drop | 1 (mM) | ||
8 | 1 | drop | dithiothreitol | 0.1 (mM) | |
9 | 1 | reservoir | imidazole-HCl | 50 (mM) |