1AK9
SUBTILISIN MUTANT 8321
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | SUBTILISIN 8321 | polymer | 275 | 27539.6 | 1 | UniProt (P00782) Pfam (PF00082) | Bacillus amyloliquefaciens | |
| 2 | B, D (A) | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
| 3 | C (A) | SODIUM ION | non-polymer | 23.0 | 1 | Chemie (NA) | |||
| 4 | E, F (A) | ISOPROPYL ALCOHOL | non-polymer | 60.1 | 2 | Chemie (IPA) | |||
| 5 | G (A) | water | water | 18.0 | 198 | Chemie (HOH) |
Sequence modifications
A: 1 - 275 (UniProt: P00782)
| PDB | External Database | Details |
|---|---|---|
| Cys 22 | Thr 129 | engineered mutation |
| Phe 50 | Met 157 | engineered mutation |
| Cys 87 | Ser 194 | engineered mutation |
| Ala 169 | Gly 276 | engineered mutation |
| Lys 217 | Tyr 324 | engineered mutation |
| Ser 218 | Asn 325 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 27539.6 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 223.3 | |
| All* | Total formula weight | 27763.0 |
