12PB

Single particle cryo-EM structure of human MTCH2 (hyperactive mutant K25E Y235A V238D)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	UCP1 Nanobody	polymer	127	13913.5	1		synthetic construct
2	B (A)	Mitochondrial carrier homolog 2,Mitochondrial brown fat uncoupling protein 1	polymer	304	33481.0	1	UniProt (Q9Y6C9) UniProt (P25874)	Homo sapiens (human)	Met-induced mitochondrial protein,UCP 1,Solute carrier family 25 member 7,Thermogenin

Sequence modifications

A: 1 - 202 (UniProt: Q9Y6C9)

PDB	External Database	Details
Glu 25	Lys 25	engineered mutation
Val 199	Leu 199	engineered mutation
Tyr 200	Ala 200	engineered mutation

A: 203 - 222 (UniProt: P25874)
A: 223 - 304 (UniProt: Q9Y6C9)

PDB	External Database	Details
Ala 227	Thr 226	engineered mutation
Ala 236	Tyr 235	engineered mutation
Asp 239	Val 238	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	47394.5
All*	Total formula weight	47394.5

*Water molecules are not included.