12OZ

Single particle cryo-EM structure of human MTCH2 (hyperactive mutant F285N F286N)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Mitochondrial carrier homolog 2,Mitochondrial brown fat uncoupling protein 1	polymer	304	33491.1	1	UniProt (Q9Y6C9) UniProt (P25874)	Homo sapiens (human)	Met-induced mitochondrial protein,UCP 1,Solute carrier family 25 member 7,Thermogenin
2	B (B)	UCP1 Nanobody	polymer	127	13913.5	1		synthetic construct

Sequence modifications

A: 1 - 202 (UniProt: Q9Y6C9)

PDB	External Database	Details
Val 199	Leu 199	engineered mutation
Tyr 200	Ala 200	engineered mutation

A: 203 - 222 (UniProt: P25874)
A: 223 - 304 (UniProt: Q9Y6C9)

PDB	External Database	Details
Ala 227	Thr 226	engineered mutation
Asn 286	Phe 285	engineered mutation
Asn 287	Phe 286	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	47404.6
All*	Total formula weight	47404.6

*Water molecules are not included.