11SS

Cryo-EM structure of DNA polymerase Zeta with RNA:DNA hybrid and incoming dNTP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA polymerase zeta catalytic subunit	polymer	1538	177068.5	1	UniProt (P14284)	Saccharomyces cerevisiae (brewer's yeast)	Protein reversionless 3
2	B, C (D, E)	DNA polymerase zeta processivity subunit	polymer	245	28791.7	2	UniProt (P38927)	Saccharomyces cerevisiae (brewer's yeast)	Revertibility protein 7
3	D (G)	DNA polymerase delta subunit 3	polymer	350	40377.7	1	UniProt (P47110)	Saccharomyces cerevisiae (brewer's yeast)
4	E (B)	DNA repair protein REV1	polymer	985	112384.2	1	UniProt (P12689)	Saccharomyces cerevisiae (brewer's yeast)	Reversionless protein 1
5	F (P)	DNA (5'-D(P*AP*TP*TP*AP*CP*GP*AP*AP*TP*TP*GP*C)-3')	polymer	20	6133.0	1		synthetic construct
6	G (F)	DNA polymerase delta small subunit	polymer	494	55987.4	1	UniProt (P46957)	Saccharomyces cerevisiae (brewer's yeast)	Hydroxyurea-sensitive protein 2
7	H (X)	RNA (5'-R(P*AP*AP*UP*GP*GP*CP*AP*AP*UP*UP*CP*GP*UP*AP*AP*U)-3')	polymer	25	7984.8	1		synthetic construct
8	I (A)	CALCIUM ION	non-polymer		40.1	1	Chemie (CA) PubChem (271)
9	J (A)	2'-DEOXYCYTIDINE-5'-TRIPHOSPHATE	non-polymer		467.2	1	Chemie (DCP)
10	K (A)	IRON/SULFUR CLUSTER	non-polymer		351.6	1	Chemie (SF4)
11	L, M (A, D)	water	water		18.0	7	Chemie (HOH)

Sequence modifications

A: 1 - 1504 (UniProt: P14284)

PDB	External Database	Details
Met -33	-	initiating methionine
Asp -32	-	expression tag
Tyr -31	-	expression tag
Lys -30	-	expression tag
Asp -29	-	expression tag
Asp -28	-	expression tag
Asp -27	-	expression tag
Asp -26	-	expression tag
Lys -25	-	expression tag
Gly -24	-	expression tag
Asp -23	-	expression tag
His -22	-	expression tag
Asn -21	-	expression tag
His -20	-	expression tag
Arg -19	-	expression tag
His -18	-	expression tag
Lys -17	-	expression tag
His -16	-	expression tag
Gly -15	-	expression tag
Asp -14	-	expression tag
Pro -13	-	expression tag
Leu -12	-	expression tag
Glu -11	-	expression tag
Val -10	-	expression tag
Leu -9	-	expression tag
Phe -8	-	expression tag
Gln -7	-	expression tag
Gly -6	-	expression tag
Pro -5	-	expression tag
Gly -4	-	expression tag
Gly -3	-	expression tag
Asp -2	-	expression tag
Pro -1	-	expression tag
His 0	-	expression tag

F: 1 - 487 (UniProt: P46957)

PDB	External Database	Details
Gly -6	-	expression tag
Pro -5	-	expression tag
Gly -4	-	expression tag
Gly -3	-	expression tag
Asp -2	-	expression tag
Leu -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	457518.9
Non-Polymers*	Number of molecules	3
	Total formula weight	858.9
All*	Total formula weight	458377.7

*Water molecules are not included.