11GK
Human Argonaute2 R315V/H316A - guide RNA in complex with a fully complementary target (conformation 2)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Argonaute-2 R315V/H316A | polymer | 859 | 97277.1 | 1 | UniProt (Q9UKV8) | Homo sapiens (human) | Argonaute2,hAgo2,Argonaute RISC catalytic component 2,Eukaryotic translation initiation factor 2C 2,eIF-2C 2,eIF2C 2,PAZ Piwi domain protein,PPD,Protein slicer |
| 2 | B (B) | guide RNA | polymer | 20 | 6481.9 | 1 | synthetic construct | ||
| 3 | C (C) | target RNA | polymer | 23 | 7258.4 | 1 | synthetic construct | ||
| 4 | D (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) |
Sequence modifications
A: 1 - 859 (UniProt: Q9UKV8)
| PDB | External Database | Details |
|---|---|---|
| Val 315 | Arg 315 | engineered mutation |
| Ala 316 | His 316 | engineered mutation |
| Asp 387 | Ser 387 | engineered mutation |
| Ala 824 | Ser 824 | engineered mutation |
| Asp 828 | Ser 828 | engineered mutation |
| Asp 831 | Ser 831 | engineered mutation |
| Ala 834 | Ser 834 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 111017.3 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 24.3 | |
| All* | Total formula weight | 111041.6 |
