11GK
Human Argonaute2 R315V/H316A - guide RNA in complex with a fully complementary target (conformation 2)
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TALOS ARCTICA |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2.7 |
| Detector | FEI FALCON IV (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3 |
| Software | cryoSPARC,PHENIX,Coot,UCSF ChimeraX,EPU |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
