10VB

Crystal structure of GeoCas9 HNH domain bound to S78A mutant anti-CRISPR AcrIIC1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G, I... (A, C, E, G, I...)	CRISPR-associated endonuclease Cas9	polymer	111	13078.8	6	UniProt (A0ABF7PG96)	Geobacillus stearothermophilus
2	B, D, F, H, J... (B, D, F, H, J...)	Anti-CRISPR protein (AcrIIC1)	polymer	86	9621.9	6	UniProt (A0A2D0TCG3)	Geobacillus stearothermophilus
3	M, N, O, P, Q... (A, B, C, D, E...)	water	water		18.0	750	Chemie (HOH)

Sequence modifications

A, C, E, G, I, K: 4 - 111 (UniProt: A0ABF7PG96)

PDB	External Database	Details
Ser 1	-	expression tag
Gly 2	-	expression tag
Gly 3	-	expression tag

B, D, F, H, J, L: 1 - 86 (UniProt: A0A2D0TCG3)

PDB	External Database	Details
Ala 79	Ser 79	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	136203.9
All*	Total formula weight	136203.9

*Water molecules are not included.