10VB
Crystal structure of GeoCas9 HNH domain bound to S78A mutant anti-CRISPR AcrIIC1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | RIGAKU MICROMAX-003 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2025-11-20 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.541780 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.478, 90.469, 87.218 |
| Unit cell angles | 90.00, 93.82, 90.00 |
Refinement procedure
| Resolution | 45.230 - 2.300 |
| R-factor | 0.213 |
| Rwork | 0.209 |
| R-free | 0.27920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.959 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (2.0_5885) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.230 | 9.760 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.368 | |
| Number of reflections | 56509 | 11548 |
| <I/σ(I)> | 3.1 | |
| Completeness [%] | 98.0 | |
| Redundancy | 3.7 | |
| CC(1/2) | 0.991 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 0.2M sodium malonate, 22.5% PEG 3350 |
