10OZ

Structure of PDE5 PDE6 chimera

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	cGMP-specific 3',5'-cyclic phosphodiesterase PDE5-PDE6 chimera	polymer	800	91715.9	2	UniProt (P16586) UniProt (Q28156) Pfam (PF01590) Pfam (PF00233)	Bos taurus (domestic cattle)	PDE V-C1,cGMP phosphodiesterase 6C,cGMP-binding cGMP-specific phosphodiesterase,CGB-PDE
2	C, D (C, D)	Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma	polymer	107	11855.6	2	UniProt (P04972) Pfam (PF04868)	Bos taurus (domestic cattle)	GMP-PDE gamma
3	E, J (A, B)	5-{2-ETHOXY-5-[(4-METHYLPIPERAZIN-1-YL)SULFONYL]PHENYL}-1-METHYL-3-PROPYL-1H,6H,7H-PYRAZOLO[4,3-D]PYRIMIDIN-7-ONE	non-polymer		474.6	2	Chemie (VIA)
4	F, I (A, B)	CYCLIC GUANOSINE MONOPHOSPHATE	non-polymer		345.2	2	Chemie (PCG)
5	G, K (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG) PubChem (888)
6	H, L (A, B)	ZINC ION	non-polymer		65.4	2	Chemie (ZN) PubChem (32051)

Sequence modifications

A, B: 1 - 440 (UniProt: P16586)
A, B: 441 - 800 (UniProt: Q28156)
C, D: 1 - 87 (UniProt: P04972)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	207143.0
Non-Polymers*	Number of molecules	8
	Total formula weight	1819.0
All*	Total formula weight	208962.0

*Water molecules are not included.