4REK
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CRYSTAL STRUCTURE AND CHARGE DENSITY STUDIES OF CHOLESTEROL OXIDASE FROM BREVIBACTERIUM STEROLICUM AT 0.74 ULTRA-HIGH RESOLUTION
Descriptor:Cholesterol oxidase, FLAVIN-ADENINE DINUCLEOTIDE, PHOSPHATE ION
Authors:Zarychta, B., Lyubimov, A., Ahmed, M., Munshi, P., Guillot, B., Vrielink, A.
Deposit date:2014-09-23
Release date:2015-04-15
Last modified:2017-11-22
Method:X-RAY DIFFRACTION (0.74 Å)
Cite:Cholesterol oxidase: ultrahigh-resolution crystal structure and multipolar atom model-based analysis.
Acta Crystallogr.,Sect.D, 71, 2015
4XXG
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STRUCTURE OF PROTONATED CHOLESTEROL OXIDASE FROM STREPTOMYCES SA-COO
Descriptor:Cholesterol oxidase, FLAVIN-ADENINE DINUCLEOTIDE
Authors:Golden, E., Vrielink, A.
Deposit date:2015-01-30
Release date:2015-12-30
Method:X-RAY DIFFRACTION (0.85 Å)
Cite:Production and characterization of recombinant perdeuterated cholesterol oxidase.
Anal.Biochem., 485, 2015
1N4W
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ATOMIC RESOLUTION STRUCTURE OF CHOLESTEROL OXIDASE @ PH 7.3 (STREPTOMYCES SP. SA-COO)
Descriptor:Cholesterol oxidase, MANGANESE (II) ION, FLAVIN-ADENINE DINUCLEOTIDE, ...
Authors:Vrielink, A., Lario, P.I.
Deposit date:2002-11-01
Release date:2004-04-27
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (0.92 Å)
Cite:Atomic resolution crystallography reveals how changes in pH shape the protein microenvironment
Nat.Chem.Biol., 2, 2006
3GYJ
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CHOLESTEROL OXIDASE FROM STREPTOMYCES SP. N485L MUTANT (0.92A)
Descriptor:Cholesterol oxidase, FLAVIN-ADENINE DINUCLEOTIDE, SULFATE ION
Authors:Lyubimov, A.Y., Vrielink, A.
Deposit date:2009-04-03
Release date:2009-12-01
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (0.92 Å)
Cite:A hydrogen-bonding network is important for oxidation and isomerization in the reaction catalyzed by cholesterol oxidase.
Acta Crystallogr.,Sect.D, 65, 2009
1MXT
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ATOMIC RESOLUTION STRUCTURE OF CHOLESTEROL OXIDASE (STREPTOMYCES SP. SA-COO)
Descriptor:CHOLESTEROL OXIDASE, SULFATE ION, FLAVIN-N7 PROTONATED-ADENINE DINUCLEOTIDE, ...
Authors:Vrielink, A., Lario, P.I.
Deposit date:2002-10-03
Release date:2003-02-25
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (0.95 Å)
Cite:Sub-atomic resolution crystal structure of cholesterol oxidase: What atomic resolution crystallography reveals about enzyme mechanism and the role of FAD cofactor in redox activity
J.Mol.Biol., 326, 2003
1N1P
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ATOMIC RESOLUTION STRUCTURE OF CHOLESTEROL OXIDASE @ PH 7.4 (STREPTOMYCES SP. SA-COO)
Descriptor:Cholesterol oxidase, MANGANESE (II) ION, FLAVIN-ADENINE DINUCLEOTIDE, ...
Authors:Vrielink, A., Lario, P.I.
Deposit date:2002-10-18
Release date:2003-10-28
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (0.95 Å)
Cite:Atomic Resolution Density Maps Reveal Secondary Structure Dependent Differences in Electronic Distribution
J.Am.Chem.Soc., 125, 2003
1N4U
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CHOLESTEROL OXIDASE FROM STREPTOMYCES @ PH 4.5 (STREPTOMYCES SP. SA-COO)
Descriptor:Cholesterol oxidase, PHOSPHATE ION, FLAVIN-N7 PROTONATED-ADENINE DINUCLEOTIDE, ...
Authors:Vrielink, A., Lario, P.I.
Deposit date:2002-11-01
Release date:2004-04-27
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (0.95 Å)
Cite:Atomic resolution crystallography reveals how changes in pH shape the protein microenvironment
Nat.Chem.Biol., 2, 2006
3CNJ
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CHOLESTEROL OXIDASE FROM STREPTOMYCES SP. F359W MUTANT (0.95A)
Descriptor:Cholesterol oxidase, SULFATE ION, FLAVIN-ADENINE DINUCLEOTIDE
Authors:Lyubimov, A.Y., Brammer, L., Vrielink, A.
Deposit date:2008-03-25
Release date:2008-04-29
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (0.95 Å)
Cite:The binding and release of oxygen and hydrogen peroxide are directed by a hydrophobic tunnel in cholesterol oxidase
Biochemistry, 47, 2008
2GEW
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ATOMIC RESOLUTION STRUCTURE OF CHOLESTEROL OXIDASE @ PH 9.0 (STREPTOMYCES SP. SA-COO)
Descriptor:Cholesterol oxidase, SULFATE ION, FLAVIN-ADENINE DINUCLEOTIDE, ...
Authors:Lyubimov, A.Y., Vrielink, A.
Deposit date:2006-03-20
Release date:2006-05-02
Last modified:2018-01-24
Method:X-RAY DIFFRACTION (0.97 Å)
Cite:Atomic resolution crystallography reveals how changes in pH shape the protein microenvironment.
Nat.Chem.Biol., 2, 2006
3B3R
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CRYSTAL STRUCTURE OF STREPTOMYCES CHOLESTEROL OXIDASE H447Q/E361Q MUTANT BOUND TO GLYCEROL (0.98A)
Descriptor:Cholesterol oxidase, SULFATE ION, FLAVIN-N7 PROTONATED-ADENINE DINUCLEOTIDE, ...
Authors:Lyubimov, A.Y., Heard, K., Tang, H., Sampson, N.S., Vrielink, A.
Deposit date:2007-10-22
Release date:2007-12-18
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (0.98 Å)
Cite:Distortion of flavin geometry is linked to ligand binding in cholesterol oxidase
Protein Sci., 16, 2007
1N4V
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ATOMIC RESOLUTION STRUCTURE OF CHOLESTEROL OXIDASE @PH 5.8 (STREPTOMYCES SP. SA-COO)
Descriptor:Cholesterol oxidase, FLAVIN-ADENINE DINUCLEOTIDE, GLYCEROL
Authors:Vrielink, A., Lario, P.I.
Deposit date:2002-11-01
Release date:2004-04-27
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (1 Å)
Cite:Atomic resolution crystallography reveals how changes in pH shape the protein microenvironment
Nat.Chem.Biol., 2, 2006
3GYI
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CHOLESTEROL OXIDASE FROM STREPTOMYCES SP. N485D MUTANT (1.0A)
Descriptor:Cholesterol oxidase, FLAVIN-ADENINE DINUCLEOTIDE, SULFATE ION
Authors:Lyubimov, A.Y., Vrielink, A.
Deposit date:2009-04-03
Release date:2009-12-01
Last modified:2017-11-01
Method:X-RAY DIFFRACTION (1 Å)
Cite:A hydrogen-bonding network is important for oxidation and isomerization in the reaction catalyzed by cholesterol oxidase.
Acta Crystallogr.,Sect.D, 65, 2009
4XWR
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X-RAY STRUCTURE OF PERDEUTERATED CHOLESTEROL OXIDASE FROM STREPTOMYCES SA-COO
Descriptor:Cholesterol oxidase, FLAVIN-ADENINE DINUCLEOTIDE, SULFATE ION
Authors:Golden, E., Vrielink, A.
Deposit date:2015-01-29
Release date:2015-12-30
Method:X-RAY DIFFRACTION (1.1 Å)
Cite:Production and characterization of recombinant perdeuterated cholesterol oxidase.
Anal.Biochem., 485, 2015
4U2S
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CHOLESTEROL OXIDASE IN THE REDUCED STATE COMPLEXED WITH ISOPROPANOL
Descriptor:Cholesterol oxidase, SULFATE ION, DIHYDROFLAVINE-ADENINE DINUCLEOTIDE
Authors:Golden, E.A., Vrielink, A.
Deposit date:2014-07-18
Release date:2014-12-10
Last modified:2017-11-22
Method:X-RAY DIFFRACTION (1.12 Å)
Cite:High-resolution structures of cholesterol oxidase in the reduced state provide insights into redox stabilization.
Acta Crystallogr.,Sect.D, 70, 2014
3B6D
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CRYSTAL STRUCTURE OF STREPTOMYCES CHOLESTEROL OXIDASE H447Q/E361Q MUTANT (1.2A)
Descriptor:Cholesterol oxidase, SULFATE ION, FLAVIN-N7 PROTONATED-ADENINE DINUCLEOTIDE
Authors:Lyubimov, A.Y., Vrielink, A.
Deposit date:2007-10-29
Release date:2007-12-18
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (1.2 Å)
Cite:Distortion of flavin geometry is linked to ligand binding in cholesterol oxidase
Protein Sci., 16, 2007
3QVP
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CRYSTAL STRUCTURE OF GLUCOSE OXIDASE FOR SPACE GROUP C2221 AT 1.2 A RESOLUTION
Descriptor:Glucose oxidase, N-ACETYL-D-GLUCOSAMINE, BETA-D-MANNOSE, ...
Authors:Kommoju, P., Chen, Z., Bruckner, R.C., Mathews, F.S., Jorns, M.S.
Deposit date:2011-02-25
Release date:2011-06-01
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (1.2 Å)
Cite:Probing oxygen activation sites in two flavoprotein oxidases using chloride as an oxygen surrogate.
Biochemistry, 50, 2011
4U2T
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CHOLESTEROL OXIDASE IN THE OXIDISED STATE COMPLEXED WITH ISOPROPANOL
Descriptor:Cholesterol oxidase, SULFATE ION, FLAVIN-ADENINE DINUCLEOTIDE
Authors:Golden, E.A., Vrielink, A.
Deposit date:2014-07-18
Release date:2014-12-10
Last modified:2017-11-22
Method:X-RAY DIFFRACTION (1.223 Å)
Cite:High-resolution structures of cholesterol oxidase in the reduced state provide insights into redox stabilization.
Acta Crystallogr.,Sect.D, 70, 2014
3QVR
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CRYSTAL STRUCTURE OF GLUCOSE OXIDASE FOR SPACE GROUP P3121 AT 1.3 A RESOLUTION.
Descriptor:Glucose oxidase, N-ACETYL-D-GLUCOSAMINE, BETA-D-MANNOSE, ...
Authors:Kommoju, P., Chen, Z., Bruckner, R.C., Mathews, F.S., Jorns, M.S.
Deposit date:2011-02-25
Release date:2011-06-01
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (1.3 Å)
Cite:Probing oxygen activation sites in two flavoprotein oxidases using chloride as an oxygen surrogate.
Biochemistry, 50, 2011
4U2L
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DITHIONITE REDUCED CHOLESTEROL IN COMPLEX WITH SULFITE
Descriptor:Cholesterol oxidase, SULFATE ION, (S)-10-((2S,3S,4R)-5-((S)-((S)-(((2R,3S,4R,5R)-5-(6-AMINO-9H-PURIN-9-YL)-3,4-DIHYDROXY-TETRAHYDROFURAN-2-YL)METHOXY)(HYDROXY)PHOSPHORYLOXY)(HYDROXY)PHOSPHORYLOXY)-2,3,4-TRIHYDROXYPENTYL)-7,8-DIMETHYL-2,4-DIOXO-2,3,4,4A-TETRAHYDROBENZO[G]PTERIDINE-5(10H)-SULFONIC ACID
Authors:Golden, E.A., Vrielink, A.
Deposit date:2014-07-17
Release date:2014-12-10
Last modified:2017-11-22
Method:X-RAY DIFFRACTION (1.337 Å)
Cite:High-resolution structures of cholesterol oxidase in the reduced state provide insights into redox stabilization.
Acta Crystallogr.,Sect.D, 70, 2014
1JU2
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CRYSTAL STRUCTURE OF THE HYDROXYNITRILE LYASE FROM ALMOND
Descriptor:hydroxynitrile lyase, N-ACETYL-D-GLUCOSAMINE, 2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE, ...
Authors:Dreveny, I., Gruber, K., Glieder, A., Thompson, A., Kratky, C.
Deposit date:2001-08-23
Release date:2002-09-04
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (1.47 Å)
Cite:The hydroxynitrile lyase from almond: a lyase that looks like an oxidoreductase.
Structure, 9, 2001
5KWF
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JOINT X-RAY NEUTRON STRUCTURE OF CHOLESTEROL OXIDASE
Descriptor:Cholesterol oxidase, FLAVIN-ADENINE DINUCLEOTIDE
Authors:Golden, E., Vrielink, A., Meilleur, F., Blakeley, M.
Deposit date:2016-07-18
Release date:2017-02-01
Last modified:2017-09-27
Method:X-RAY DIFFRACTION (1.499 Å), NEUTRON DIFFRACTION
Cite:An extended N-H bond, driven by a conserved second-order interaction, orients the flavin N5 orbital in cholesterol oxidase.
Sci Rep, 7, 2017
1B4V
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CHOLESTEROL OXIDASE FROM STREPTOMYCES
Descriptor:PROTEIN (CHOLESTEROL OXIDASE), FLAVIN-ADENINE DINUCLEOTIDE
Authors:Vrielink, A., Yue, Q.K.
Deposit date:1998-12-30
Release date:1999-01-06
Last modified:2017-10-04
Method:X-RAY DIFFRACTION (1.5 Å)
Cite:Crystal structure determination of cholesterol oxidase from Streptomyces and structural characterization of key active site mutants.
Biochemistry, 38, 1999
1KDG
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CRYSTAL STRUCTURE OF THE FLAVIN DOMAIN OF CELLOBIOSE DEHYDROGENASE
Descriptor:cellobiose dehydrogenase, N-ACETYL-D-GLUCOSAMINE, ALPHA-D-MANNOSE, ...
Authors:Hallberg, B.M., Henriksson, G., Pettersson, G., Divne, C.
Deposit date:2001-11-13
Release date:2002-11-13
Last modified:2018-03-07
Method:X-RAY DIFFRACTION (1.5 Å)
Cite:Crystal Structure of the Flavoprotein Domain of the Extracellular Flavocytochrome Cellobiose Dehydrogenase
J.Mol.Biol., 315, 2002
1IJH
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CHOLESTEROL OXIDASE FROM STREPTOMYCES ASN485LEU MUTANT
Descriptor:CHOLESTEROL OXIDASE, FLAVIN-ADENINE DINUCLEOTIDE
Authors:Vrielink, A., Lario, P.I.
Deposit date:2001-04-26
Release date:2001-12-28
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (1.53 Å)
Cite:The presence of a hydrogen bond between asparagine 485 and the pi system of FAD modulates the redox potential in the reaction catalyzed by cholesterol oxidase.
Biochemistry, 40, 2001
3GDP
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HYDROXYNITRILE LYASE FROM ALMOND, MONOCLINIC CRYSTAL FORM
Descriptor:R-oxynitrile lyase isoenzyme 1, ISOPROPYL ALCOHOL, FLAVIN-ADENINE DINUCLEOTIDE, ...
Authors:Dreveny, I., Gruber, K., Kratky, C.
Deposit date:2009-02-24
Release date:2009-03-24
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (1.57 Å)
Cite:Substrate binding in the FAD-dependent hydroxynitrile lyase from almond provides insight into the mechanism of cyanohydrin formation and explains the absence of dehydrogenation activity.
Biochemistry, 48, 2009
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