9ZBL

Helical Reconstruction of the Human Cardiac F-Actin-Tropomyosin Complex

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000146	molecular_function	microfilament motor activity
A	0005200	molecular_function	structural constituent of cytoskeleton
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005576	cellular_component	extracellular region
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0005856	cellular_component	cytoskeleton
A	0005884	cellular_component	actin filament
A	0005925	cellular_component	focal adhesion
A	0007015	biological_process	actin filament organization
A	0010628	biological_process	positive regulation of gene expression
A	0015629	cellular_component	actin cytoskeleton
A	0016020	cellular_component	membrane
A	0016887	molecular_function	ATP hydrolysis activity
A	0017022	molecular_function	myosin binding
A	0030017	cellular_component	sarcomere
A	0030027	cellular_component	lamellipodium
A	0030048	biological_process	actin filament-based movement
A	0030175	cellular_component	filopodium
A	0030240	biological_process	skeletal muscle thin filament assembly
A	0031032	biological_process	actomyosin structure organization
A	0031674	cellular_component	I band
A	0033275	biological_process	actin-myosin filament sliding
A	0044297	cellular_component	cell body
A	0045202	cellular_component	synapse
A	0055003	biological_process	cardiac myofibril assembly
A	0055008	biological_process	cardiac muscle tissue morphogenesis
A	0060047	biological_process	heart contraction
A	0070062	cellular_component	extracellular exosome
A	0072562	cellular_component	blood microparticle
A	0090131	biological_process	mesenchyme migration
A	0098978	cellular_component	glutamatergic synapse
B	0000146	molecular_function	microfilament motor activity
B	0005200	molecular_function	structural constituent of cytoskeleton
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005576	cellular_component	extracellular region
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0005856	cellular_component	cytoskeleton
B	0005884	cellular_component	actin filament
B	0005925	cellular_component	focal adhesion
B	0007015	biological_process	actin filament organization
B	0010628	biological_process	positive regulation of gene expression
B	0015629	cellular_component	actin cytoskeleton
B	0016020	cellular_component	membrane
B	0016887	molecular_function	ATP hydrolysis activity
B	0017022	molecular_function	myosin binding
B	0030017	cellular_component	sarcomere
B	0030027	cellular_component	lamellipodium
B	0030048	biological_process	actin filament-based movement
B	0030175	cellular_component	filopodium
B	0030240	biological_process	skeletal muscle thin filament assembly
B	0031032	biological_process	actomyosin structure organization
B	0031674	cellular_component	I band
B	0033275	biological_process	actin-myosin filament sliding
B	0044297	cellular_component	cell body
B	0045202	cellular_component	synapse
B	0055003	biological_process	cardiac myofibril assembly
B	0055008	biological_process	cardiac muscle tissue morphogenesis
B	0060047	biological_process	heart contraction
B	0070062	cellular_component	extracellular exosome
B	0072562	cellular_component	blood microparticle
B	0090131	biological_process	mesenchyme migration
B	0098978	cellular_component	glutamatergic synapse
C	0000146	molecular_function	microfilament motor activity
C	0005200	molecular_function	structural constituent of cytoskeleton
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005576	cellular_component	extracellular region
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0005856	cellular_component	cytoskeleton
C	0005884	cellular_component	actin filament
C	0005925	cellular_component	focal adhesion
C	0007015	biological_process	actin filament organization
C	0010628	biological_process	positive regulation of gene expression
C	0015629	cellular_component	actin cytoskeleton
C	0016020	cellular_component	membrane
C	0016887	molecular_function	ATP hydrolysis activity
C	0017022	molecular_function	myosin binding
C	0030017	cellular_component	sarcomere
C	0030027	cellular_component	lamellipodium
C	0030048	biological_process	actin filament-based movement
C	0030175	cellular_component	filopodium
C	0030240	biological_process	skeletal muscle thin filament assembly
C	0031032	biological_process	actomyosin structure organization
C	0031674	cellular_component	I band
C	0033275	biological_process	actin-myosin filament sliding
C	0044297	cellular_component	cell body
C	0045202	cellular_component	synapse
C	0055003	biological_process	cardiac myofibril assembly
C	0055008	biological_process	cardiac muscle tissue morphogenesis
C	0060047	biological_process	heart contraction
C	0070062	cellular_component	extracellular exosome
C	0072562	cellular_component	blood microparticle
C	0090131	biological_process	mesenchyme migration
C	0098978	cellular_component	glutamatergic synapse
D	0000146	molecular_function	microfilament motor activity
D	0005200	molecular_function	structural constituent of cytoskeleton
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0005576	cellular_component	extracellular region
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0005856	cellular_component	cytoskeleton
D	0005884	cellular_component	actin filament
D	0005925	cellular_component	focal adhesion
D	0007015	biological_process	actin filament organization
D	0010628	biological_process	positive regulation of gene expression
D	0015629	cellular_component	actin cytoskeleton
D	0016020	cellular_component	membrane
D	0016887	molecular_function	ATP hydrolysis activity
D	0017022	molecular_function	myosin binding
D	0030017	cellular_component	sarcomere
D	0030027	cellular_component	lamellipodium
D	0030048	biological_process	actin filament-based movement
D	0030175	cellular_component	filopodium
D	0030240	biological_process	skeletal muscle thin filament assembly
D	0031032	biological_process	actomyosin structure organization
D	0031674	cellular_component	I band
D	0033275	biological_process	actin-myosin filament sliding
D	0044297	cellular_component	cell body
D	0045202	cellular_component	synapse
D	0055003	biological_process	cardiac myofibril assembly
D	0055008	biological_process	cardiac muscle tissue morphogenesis
D	0060047	biological_process	heart contraction
D	0070062	cellular_component	extracellular exosome
D	0072562	cellular_component	blood microparticle
D	0090131	biological_process	mesenchyme migration
D	0098978	cellular_component	glutamatergic synapse
E	0000146	molecular_function	microfilament motor activity
E	0005200	molecular_function	structural constituent of cytoskeleton
E	0005515	molecular_function	protein binding
E	0005524	molecular_function	ATP binding
E	0005576	cellular_component	extracellular region
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0005856	cellular_component	cytoskeleton
E	0005884	cellular_component	actin filament
E	0005925	cellular_component	focal adhesion
E	0007015	biological_process	actin filament organization
E	0010628	biological_process	positive regulation of gene expression
E	0015629	cellular_component	actin cytoskeleton
E	0016020	cellular_component	membrane
E	0016887	molecular_function	ATP hydrolysis activity
E	0017022	molecular_function	myosin binding
E	0030017	cellular_component	sarcomere
E	0030027	cellular_component	lamellipodium
E	0030048	biological_process	actin filament-based movement
E	0030175	cellular_component	filopodium
E	0030240	biological_process	skeletal muscle thin filament assembly
E	0031032	biological_process	actomyosin structure organization
E	0031674	cellular_component	I band
E	0033275	biological_process	actin-myosin filament sliding
E	0044297	cellular_component	cell body
E	0045202	cellular_component	synapse
E	0055003	biological_process	cardiac myofibril assembly
E	0055008	biological_process	cardiac muscle tissue morphogenesis
E	0060047	biological_process	heart contraction
E	0070062	cellular_component	extracellular exosome
E	0072562	cellular_component	blood microparticle
E	0090131	biological_process	mesenchyme migration
E	0098978	cellular_component	glutamatergic synapse
F	0000146	molecular_function	microfilament motor activity
F	0005200	molecular_function	structural constituent of cytoskeleton
F	0005515	molecular_function	protein binding
F	0005524	molecular_function	ATP binding
F	0005576	cellular_component	extracellular region
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0005856	cellular_component	cytoskeleton
F	0005884	cellular_component	actin filament
F	0005925	cellular_component	focal adhesion
F	0007015	biological_process	actin filament organization
F	0010628	biological_process	positive regulation of gene expression
F	0015629	cellular_component	actin cytoskeleton
F	0016020	cellular_component	membrane
F	0016887	molecular_function	ATP hydrolysis activity
F	0017022	molecular_function	myosin binding
F	0030017	cellular_component	sarcomere
F	0030027	cellular_component	lamellipodium
F	0030048	biological_process	actin filament-based movement
F	0030175	cellular_component	filopodium
F	0030240	biological_process	skeletal muscle thin filament assembly
F	0031032	biological_process	actomyosin structure organization
F	0031674	cellular_component	I band
F	0033275	biological_process	actin-myosin filament sliding
F	0044297	cellular_component	cell body
F	0045202	cellular_component	synapse
F	0055003	biological_process	cardiac myofibril assembly
F	0055008	biological_process	cardiac muscle tissue morphogenesis
F	0060047	biological_process	heart contraction
F	0070062	cellular_component	extracellular exosome
F	0072562	cellular_component	blood microparticle
F	0090131	biological_process	mesenchyme migration
F	0098978	cellular_component	glutamatergic synapse
M	0001725	cellular_component	stress fiber
M	0003065	biological_process	positive regulation of heart rate by epinephrine
M	0003779	molecular_function	actin binding
M	0005200	molecular_function	structural constituent of cytoskeleton
M	0005515	molecular_function	protein binding
M	0005829	cellular_component	cytosol
M	0005856	cellular_component	cytoskeleton
M	0005862	cellular_component	muscle thin filament tropomyosin
M	0005884	cellular_component	actin filament
M	0006937	biological_process	regulation of muscle contraction
M	0007010	biological_process	cytoskeleton organization
M	0007015	biological_process	actin filament organization
M	0008016	biological_process	regulation of heart contraction
M	0008092	molecular_function	cytoskeletal protein binding
M	0008307	molecular_function	structural constituent of muscle
M	0008360	biological_process	regulation of cell shape
M	0030017	cellular_component	sarcomere
M	0030049	biological_process	muscle filament sliding
M	0030336	biological_process	negative regulation of cell migration
M	0031529	biological_process	ruffle organization
M	0032059	cellular_component	bleb
M	0032587	cellular_component	ruffle membrane
M	0034614	biological_process	cellular response to reactive oxygen species
M	0042060	biological_process	wound healing
M	0042802	molecular_function	identical protein binding
M	0042803	molecular_function	protein homodimerization activity
M	0045214	biological_process	sarcomere organization
M	0045785	biological_process	positive regulation of cell adhesion
M	0046982	molecular_function	protein heterodimerization activity
M	0051015	molecular_function	actin filament binding
M	0051496	biological_process	positive regulation of stress fiber assembly
M	0055010	biological_process	ventricular cardiac muscle tissue morphogenesis
M	0060048	biological_process	cardiac muscle contraction
M	1904706	biological_process	negative regulation of vascular associated smooth muscle cell proliferation
M	1904753	biological_process	negative regulation of vascular associated smooth muscle cell migration
N	0001725	cellular_component	stress fiber
N	0003065	biological_process	positive regulation of heart rate by epinephrine
N	0003779	molecular_function	actin binding
N	0005200	molecular_function	structural constituent of cytoskeleton
N	0005515	molecular_function	protein binding
N	0005829	cellular_component	cytosol
N	0005856	cellular_component	cytoskeleton
N	0005862	cellular_component	muscle thin filament tropomyosin
N	0005884	cellular_component	actin filament
N	0006937	biological_process	regulation of muscle contraction
N	0007010	biological_process	cytoskeleton organization
N	0007015	biological_process	actin filament organization
N	0008016	biological_process	regulation of heart contraction
N	0008092	molecular_function	cytoskeletal protein binding
N	0008307	molecular_function	structural constituent of muscle
N	0008360	biological_process	regulation of cell shape
N	0030017	cellular_component	sarcomere
N	0030049	biological_process	muscle filament sliding
N	0030336	biological_process	negative regulation of cell migration
N	0031529	biological_process	ruffle organization
N	0032059	cellular_component	bleb
N	0032587	cellular_component	ruffle membrane
N	0034614	biological_process	cellular response to reactive oxygen species
N	0042060	biological_process	wound healing
N	0042802	molecular_function	identical protein binding
N	0042803	molecular_function	protein homodimerization activity
N	0045214	biological_process	sarcomere organization
N	0045785	biological_process	positive regulation of cell adhesion
N	0046982	molecular_function	protein heterodimerization activity
N	0051015	molecular_function	actin filament binding
N	0051496	biological_process	positive regulation of stress fiber assembly
N	0055010	biological_process	ventricular cardiac muscle tissue morphogenesis
N	0060048	biological_process	cardiac muscle contraction
N	1904706	biological_process	negative regulation of vascular associated smooth muscle cell proliferation
N	1904753	biological_process	negative regulation of vascular associated smooth muscle cell migration
O	0001725	cellular_component	stress fiber
O	0003065	biological_process	positive regulation of heart rate by epinephrine
O	0003779	molecular_function	actin binding
O	0005200	molecular_function	structural constituent of cytoskeleton
O	0005515	molecular_function	protein binding
O	0005829	cellular_component	cytosol
O	0005856	cellular_component	cytoskeleton
O	0005862	cellular_component	muscle thin filament tropomyosin
O	0005884	cellular_component	actin filament
O	0006937	biological_process	regulation of muscle contraction
O	0007010	biological_process	cytoskeleton organization
O	0007015	biological_process	actin filament organization
O	0008016	biological_process	regulation of heart contraction
O	0008092	molecular_function	cytoskeletal protein binding
O	0008307	molecular_function	structural constituent of muscle
O	0008360	biological_process	regulation of cell shape
O	0030017	cellular_component	sarcomere
O	0030049	biological_process	muscle filament sliding
O	0030336	biological_process	negative regulation of cell migration
O	0031529	biological_process	ruffle organization
O	0032059	cellular_component	bleb
O	0032587	cellular_component	ruffle membrane
O	0034614	biological_process	cellular response to reactive oxygen species
O	0042060	biological_process	wound healing
O	0042802	molecular_function	identical protein binding
O	0042803	molecular_function	protein homodimerization activity
O	0045214	biological_process	sarcomere organization
O	0045785	biological_process	positive regulation of cell adhesion
O	0046982	molecular_function	protein heterodimerization activity
O	0051015	molecular_function	actin filament binding
O	0051496	biological_process	positive regulation of stress fiber assembly
O	0055010	biological_process	ventricular cardiac muscle tissue morphogenesis
O	0060048	biological_process	cardiac muscle contraction
O	1904706	biological_process	negative regulation of vascular associated smooth muscle cell proliferation
O	1904753	biological_process	negative regulation of vascular associated smooth muscle cell migration
P	0001725	cellular_component	stress fiber
P	0003065	biological_process	positive regulation of heart rate by epinephrine
P	0003779	molecular_function	actin binding
P	0005200	molecular_function	structural constituent of cytoskeleton
P	0005515	molecular_function	protein binding
P	0005829	cellular_component	cytosol
P	0005856	cellular_component	cytoskeleton
P	0005862	cellular_component	muscle thin filament tropomyosin
P	0005884	cellular_component	actin filament
P	0006937	biological_process	regulation of muscle contraction
P	0007010	biological_process	cytoskeleton organization
P	0007015	biological_process	actin filament organization
P	0008016	biological_process	regulation of heart contraction
P	0008092	molecular_function	cytoskeletal protein binding
P	0008307	molecular_function	structural constituent of muscle
P	0008360	biological_process	regulation of cell shape
P	0030017	cellular_component	sarcomere
P	0030049	biological_process	muscle filament sliding
P	0030336	biological_process	negative regulation of cell migration
P	0031529	biological_process	ruffle organization
P	0032059	cellular_component	bleb
P	0032587	cellular_component	ruffle membrane
P	0034614	biological_process	cellular response to reactive oxygen species
P	0042060	biological_process	wound healing
P	0042802	molecular_function	identical protein binding
P	0042803	molecular_function	protein homodimerization activity
P	0045214	biological_process	sarcomere organization
P	0045785	biological_process	positive regulation of cell adhesion
P	0046982	molecular_function	protein heterodimerization activity
P	0051015	molecular_function	actin filament binding
P	0051496	biological_process	positive regulation of stress fiber assembly
P	0055010	biological_process	ventricular cardiac muscle tissue morphogenesis
P	0060048	biological_process	cardiac muscle contraction
P	1904706	biological_process	negative regulation of vascular associated smooth muscle cell proliferation
P	1904753	biological_process	negative regulation of vascular associated smooth muscle cell migration

Functional Information from PROSITE/UniProt

site_id	PS00326
Number of Residues	9
Details	TROPOMYOSIN Tropomyosins signature. LKEAEtRAE

Chain	Residue	Details
M	LEU232-GLU240

---

site_id	PS00406
Number of Residues	11
Details	ACTINS_1 Actins signature 1. YVGDEAQs.KRG

Chain	Residue	Details
A	TYR53-GLY63

---

site_id	PS00432
Number of Residues	9
Details	ACTINS_2 Actins signature 2. WISKqEYDE

Chain	Residue	Details
A	TRP356-GLU364

---

site_id	PS01132
Number of Residues	13
Details	ACTINS_ACT_LIKE Actins and actin-related proteins signature. LLTEApLNPkaNR

Chain	Residue	Details
A	LEU104-ARG116

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	12
Details	Modified residue: {"description":"Methionine (R)-sulfoxide","evidences":[{"source":"UniProtKB","id":"P68033","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	6
Details	Modified residue: {"description":"Tele-methylhistidine","evidences":[{"source":"PubMed","id":"30626964","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	6
Details	Modified residue: {"description":"N6-methyllysine","evidences":[{"source":"PubMed","id":"23673617","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	6
Details	Cross-link: {"description":"Isoglutamyl lysine isopeptide (Lys-Glu) (interchain with E-272); by Vibrio toxins RtxA and VgrG1","evidences":[{"source":"UniProtKB","id":"P60709","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	12
Details	Cross-link: {"description":"Isoglutamyl lysine isopeptide (Glu-Lys) (interchain with K-52); by Vibrio toxins RtxA and VgrG1","evidences":[{"source":"UniProtKB","id":"P60709","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	80
Details	Region: {"description":"Disordered","evidences":[{"source":"SAM","id":"MobiDB-lite","evidenceCode":"ECO:0000256"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	4
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"24275569","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	8
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"P58771","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	4
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"19608861","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---