9ZBL
Helical Reconstruction of the Human Cardiac F-Actin-Tropomyosin Complex
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS GLACIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2.7 |
| Energy Filter | TFS Selectris |
| Phase Plate | OTHER |
| Detector | FEI FALCON IV (4k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 2.79 |
| Number of Particle | 2655073 |
| Software | cryoSPARC,PHENIX,MDFF |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
