9WJ6

An integrated in silico-in vitro workflow for discovering high-affinity, selective antibodies to the KRAS(G12D)-MHC I complex

Functional Information from GO Data

Chain	GOid	namespace	contents
F	0000139	cellular_component	Golgi membrane
F	0002477	biological_process	antigen processing and presentation of exogenous peptide antigen via MHC class Ib
F	0002502	biological_process	peptide antigen assembly with MHC class I protein complex
F	0002503	biological_process	peptide antigen assembly with MHC class II protein complex
F	0002715	biological_process	regulation of natural killer cell mediated immunity
F	0002726	biological_process	positive regulation of T cell cytokine production
F	0005198	molecular_function	structural molecule activity
F	0005515	molecular_function	protein binding
F	0005576	cellular_component	extracellular region
F	0005765	cellular_component	lysosomal membrane
F	0005783	cellular_component	endoplasmic reticulum
F	0005788	cellular_component	endoplasmic reticulum lumen
F	0005794	cellular_component	Golgi apparatus
F	0005886	cellular_component	plasma membrane
F	0005925	cellular_component	focal adhesion
F	0006879	biological_process	intracellular iron ion homeostasis
F	0007608	biological_process	sensory perception of smell
F	0007611	biological_process	learning or memory
F	0009986	cellular_component	cell surface
F	0010038	biological_process	response to metal ion
F	0012507	cellular_component	ER to Golgi transport vesicle membrane
F	0016020	cellular_component	membrane
F	0019885	biological_process	antigen processing and presentation of endogenous peptide antigen via MHC class I
F	0019886	biological_process	antigen processing and presentation of exogenous peptide antigen via MHC class II
F	0023026	molecular_function	MHC class II protein complex binding
F	0030670	cellular_component	phagocytic vesicle membrane
F	0031901	cellular_component	early endosome membrane
F	0031902	cellular_component	late endosome membrane
F	0031905	cellular_component	early endosome lumen
F	0032398	cellular_component	MHC class Ib protein complex
F	0033572	biological_process	transferrin transport
F	0034756	biological_process	regulation of iron ion transport
F	0034757	biological_process	negative regulation of iron ion transport
F	0035580	cellular_component	specific granule lumen
F	0042605	molecular_function	peptide antigen binding
F	0042612	cellular_component	MHC class I protein complex
F	0042613	cellular_component	MHC class II protein complex
F	0042802	molecular_function	identical protein binding
F	0042803	molecular_function	protein homodimerization activity
F	0042824	cellular_component	MHC class I peptide loading complex
F	0048260	biological_process	positive regulation of receptor-mediated endocytosis
F	0048261	biological_process	negative regulation of receptor-mediated endocytosis
F	0050680	biological_process	negative regulation of epithelial cell proliferation
F	0050768	biological_process	negative regulation of neurogenesis
F	0050778	biological_process	positive regulation of immune response
F	0050870	biological_process	positive regulation of T cell activation
F	0051289	biological_process	protein homotetramerization
F	0055038	cellular_component	recycling endosome membrane
F	0070062	cellular_component	extracellular exosome
F	0071281	biological_process	cellular response to iron ion
F	0071316	biological_process	cellular response to nicotine
F	1904724	cellular_component	tertiary granule lumen
F	1990000	biological_process	amyloid fibril formation
F	1990712	cellular_component	HFE-transferrin receptor complex
F	2000774	biological_process	positive regulation of cellular senescence
F	2000978	biological_process	negative regulation of forebrain neuron differentiation
N	0000139	cellular_component	Golgi membrane
N	0002477	biological_process	antigen processing and presentation of exogenous peptide antigen via MHC class Ib
N	0002502	biological_process	peptide antigen assembly with MHC class I protein complex
N	0002503	biological_process	peptide antigen assembly with MHC class II protein complex
N	0002715	biological_process	regulation of natural killer cell mediated immunity
N	0002726	biological_process	positive regulation of T cell cytokine production
N	0005198	molecular_function	structural molecule activity
N	0005515	molecular_function	protein binding
N	0005576	cellular_component	extracellular region
N	0005765	cellular_component	lysosomal membrane
N	0005783	cellular_component	endoplasmic reticulum
N	0005788	cellular_component	endoplasmic reticulum lumen
N	0005794	cellular_component	Golgi apparatus
N	0005886	cellular_component	plasma membrane
N	0005925	cellular_component	focal adhesion
N	0006879	biological_process	intracellular iron ion homeostasis
N	0007608	biological_process	sensory perception of smell
N	0007611	biological_process	learning or memory
N	0009986	cellular_component	cell surface
N	0010038	biological_process	response to metal ion
N	0012507	cellular_component	ER to Golgi transport vesicle membrane
N	0016020	cellular_component	membrane
N	0019885	biological_process	antigen processing and presentation of endogenous peptide antigen via MHC class I
N	0019886	biological_process	antigen processing and presentation of exogenous peptide antigen via MHC class II
N	0023026	molecular_function	MHC class II protein complex binding
N	0030670	cellular_component	phagocytic vesicle membrane
N	0031901	cellular_component	early endosome membrane
N	0031902	cellular_component	late endosome membrane
N	0031905	cellular_component	early endosome lumen
N	0032398	cellular_component	MHC class Ib protein complex
N	0033572	biological_process	transferrin transport
N	0034756	biological_process	regulation of iron ion transport
N	0034757	biological_process	negative regulation of iron ion transport
N	0035580	cellular_component	specific granule lumen
N	0042605	molecular_function	peptide antigen binding
N	0042612	cellular_component	MHC class I protein complex
N	0042613	cellular_component	MHC class II protein complex
N	0042802	molecular_function	identical protein binding
N	0042803	molecular_function	protein homodimerization activity
N	0042824	cellular_component	MHC class I peptide loading complex
N	0048260	biological_process	positive regulation of receptor-mediated endocytosis
N	0048261	biological_process	negative regulation of receptor-mediated endocytosis
N	0050680	biological_process	negative regulation of epithelial cell proliferation
N	0050768	biological_process	negative regulation of neurogenesis
N	0050778	biological_process	positive regulation of immune response
N	0050870	biological_process	positive regulation of T cell activation
N	0051289	biological_process	protein homotetramerization
N	0055038	cellular_component	recycling endosome membrane
N	0070062	cellular_component	extracellular exosome
N	0071281	biological_process	cellular response to iron ion
N	0071316	biological_process	cellular response to nicotine
N	1904724	cellular_component	tertiary granule lumen
N	1990000	biological_process	amyloid fibril formation
N	1990712	cellular_component	HFE-transferrin receptor complex
N	2000774	biological_process	positive regulation of cellular senescence
N	2000978	biological_process	negative regulation of forebrain neuron differentiation

Functional Information from PROSITE/UniProt

site_id	PS00290
Number of Residues	7
Details	IG_MHC Immunoglobulins and major histocompatibility complex proteins signature. YACEVTH

Chain	Residue	Details
A	TYR193-HIS199
B	TYR203-HIS209
E	TYR257-HIS263
F	TYR78-HIS84

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	10
Details	Binding site: {"evidences":[{"source":"PubMed","id":"22431598","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"22566140","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"34380736","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"35522713","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"40934300","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"9MF0","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI2
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"40934300","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"9MF0","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI4
Number of Residues	176
Details	Domain: {"description":"Ig-like C1-type"}

Chain

Residue

Details

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site_id	SWS_FT_FI5
Number of Residues	2
Details	Modified residue: {"description":"Pyrrolidone carboxylic acid; in form pI 5.3","evidences":[{"source":"PubMed","id":"7554280","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI6
Number of Residues	2
Details	Glycosylation: {"description":"N-linked (Glc) (glycation) isoleucine; in hemodialysis-associated amyloidosis","evidences":[{"source":"PubMed","id":"7918443","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI7
Number of Residues	12
Details	Glycosylation: {"description":"N-linked (Glc) (glycation) lysine; in vitro","evidences":[{"source":"PubMed","id":"7918443","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

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