Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

9SRX

Cryo-EM structure of the Mlc repressor in complex with the glucose-specific IICB transporter

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003677	molecular_function	DNA binding
A	0005515	molecular_function	protein binding
A	0005737	cellular_component	cytoplasm
A	0006351	biological_process	DNA-templated transcription
A	0006355	biological_process	regulation of DNA-templated transcription
A	0016020	cellular_component	membrane
A	0042802	molecular_function	identical protein binding
B	0003677	molecular_function	DNA binding
B	0005515	molecular_function	protein binding
B	0005737	cellular_component	cytoplasm
B	0006351	biological_process	DNA-templated transcription
B	0006355	biological_process	regulation of DNA-templated transcription
B	0016020	cellular_component	membrane
B	0042802	molecular_function	identical protein binding
C	0003677	molecular_function	DNA binding
C	0005515	molecular_function	protein binding
C	0005737	cellular_component	cytoplasm
C	0006351	biological_process	DNA-templated transcription
C	0006355	biological_process	regulation of DNA-templated transcription
C	0016020	cellular_component	membrane
C	0042802	molecular_function	identical protein binding
D	0003677	molecular_function	DNA binding
D	0005515	molecular_function	protein binding
D	0005737	cellular_component	cytoplasm
D	0006351	biological_process	DNA-templated transcription
D	0006355	biological_process	regulation of DNA-templated transcription
D	0016020	cellular_component	membrane
D	0042802	molecular_function	identical protein binding
E	0005515	molecular_function	protein binding
E	0005886	cellular_component	plasma membrane
E	0006355	biological_process	regulation of DNA-templated transcription
E	0009401	biological_process	phosphoenolpyruvate-dependent sugar phosphotransferase system
E	0016020	cellular_component	membrane
E	0090564	molecular_function	protein-phosphocysteine-glucose phosphotransferase system transporter activity
E	0098708	biological_process	D-glucose import across plasma membrane
E	1902495	cellular_component	transmembrane transporter complex
E	1904659	biological_process	D-glucose transmembrane transport
F	0005515	molecular_function	protein binding
F	0005886	cellular_component	plasma membrane
F	0006355	biological_process	regulation of DNA-templated transcription
F	0009401	biological_process	phosphoenolpyruvate-dependent sugar phosphotransferase system
F	0016020	cellular_component	membrane
F	0090564	molecular_function	protein-phosphocysteine-glucose phosphotransferase system transporter activity
F	0098708	biological_process	D-glucose import across plasma membrane
F	1902495	cellular_component	transmembrane transporter complex
F	1904659	biological_process	D-glucose transmembrane transport
G	0005515	molecular_function	protein binding
G	0005886	cellular_component	plasma membrane
G	0006355	biological_process	regulation of DNA-templated transcription
G	0009401	biological_process	phosphoenolpyruvate-dependent sugar phosphotransferase system
G	0016020	cellular_component	membrane
G	0090564	molecular_function	protein-phosphocysteine-glucose phosphotransferase system transporter activity
G	0098708	biological_process	D-glucose import across plasma membrane
G	1902495	cellular_component	transmembrane transporter complex
G	1904659	biological_process	D-glucose transmembrane transport
H	0005515	molecular_function	protein binding
H	0005886	cellular_component	plasma membrane
H	0006355	biological_process	regulation of DNA-templated transcription
H	0009401	biological_process	phosphoenolpyruvate-dependent sugar phosphotransferase system
H	0016020	cellular_component	membrane
H	0090564	molecular_function	protein-phosphocysteine-glucose phosphotransferase system transporter activity
H	0098708	biological_process	D-glucose import across plasma membrane
H	1902495	cellular_component	transmembrane transporter complex
H	1904659	biological_process	D-glucose transmembrane transport
I	0005515	molecular_function	protein binding
I	0005886	cellular_component	plasma membrane
I	0006355	biological_process	regulation of DNA-templated transcription
I	0009401	biological_process	phosphoenolpyruvate-dependent sugar phosphotransferase system
I	0016020	cellular_component	membrane
I	0090564	molecular_function	protein-phosphocysteine-glucose phosphotransferase system transporter activity
I	0098708	biological_process	D-glucose import across plasma membrane
I	1902495	cellular_component	transmembrane transporter complex
I	1904659	biological_process	D-glucose transmembrane transport
J	0005515	molecular_function	protein binding
J	0005886	cellular_component	plasma membrane
J	0006355	biological_process	regulation of DNA-templated transcription
J	0009401	biological_process	phosphoenolpyruvate-dependent sugar phosphotransferase system
J	0016020	cellular_component	membrane
J	0090564	molecular_function	protein-phosphocysteine-glucose phosphotransferase system transporter activity
J	0098708	biological_process	D-glucose import across plasma membrane
J	1902495	cellular_component	transmembrane transporter complex
J	1904659	biological_process	D-glucose transmembrane transport
K	0005515	molecular_function	protein binding
K	0005886	cellular_component	plasma membrane
K	0006355	biological_process	regulation of DNA-templated transcription
K	0009401	biological_process	phosphoenolpyruvate-dependent sugar phosphotransferase system
K	0016020	cellular_component	membrane
K	0090564	molecular_function	protein-phosphocysteine-glucose phosphotransferase system transporter activity
K	0098708	biological_process	D-glucose import across plasma membrane
K	1902495	cellular_component	transmembrane transporter complex
K	1904659	biological_process	D-glucose transmembrane transport
L	0005515	molecular_function	protein binding
L	0005886	cellular_component	plasma membrane
L	0006355	biological_process	regulation of DNA-templated transcription
L	0009401	biological_process	phosphoenolpyruvate-dependent sugar phosphotransferase system
L	0016020	cellular_component	membrane
L	0090564	molecular_function	protein-phosphocysteine-glucose phosphotransferase system transporter activity
L	0098708	biological_process	D-glucose import across plasma membrane
L	1902495	cellular_component	transmembrane transporter complex
L	1904659	biological_process	D-glucose transmembrane transport

Functional Information from PROSITE/UniProt

site_id	PS01035
Number of Residues	18
Details	PTS_EIIB_TYPE_1_CYS PTS EIIB domains cysteine phosphorylation site signature. NItnldaCiTRLrVsVaD

Chain	Residue	Details
K	ASN414-ASP431

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	800
Details	Transmembrane: {"description":"Helical","evidences":[{"source":"PROSITE-ProRule","id":"PRU00426","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	292
Details	Topological domain: {"description":"Periplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	280
Details	Topological domain: {"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	4
Details	Active site: {"description":"Phosphocysteine intermediate; for EIIB activity","evidences":[{"source":"PROSITE-ProRule","id":"PRU00421","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"12716891","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"18319344","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"3129430","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	4
Details	Modified residue: {"description":"Phosphocysteine","evidences":[{"source":"PubMed","id":"8505292","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	36
Details	DNA binding: {"description":"H-T-H motif","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI7
Number of Residues	16
Details	Binding site: {"evidences":[{"source":"PubMed","id":"15929984","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"18319344","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1Z6R","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3BP8","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)