9SA7

Crystal structure of Methanocaldococcus jannaschii Malate dehydrogenase C7 mutant

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004090	molecular_function	carbonyl reductase (NADPH) activity
A	0004459	molecular_function	L-lactate dehydrogenase (NAD+) activity
A	0005737	cellular_component	cytoplasm
A	0006089	biological_process	lactate metabolic process
A	0030060	molecular_function	L-malate dehydrogenase (NAD+) activity
A	0042867	biological_process	pyruvate catabolic process
A	0046554	molecular_function	L-malate dehydrogenase (NADP+) activity
A	0102443	molecular_function	L-2-hydroxycarboxylate dehydrogenase (NAD+) activity
B	0004090	molecular_function	carbonyl reductase (NADPH) activity
B	0004459	molecular_function	L-lactate dehydrogenase (NAD+) activity
B	0005737	cellular_component	cytoplasm
B	0006089	biological_process	lactate metabolic process
B	0030060	molecular_function	L-malate dehydrogenase (NAD+) activity
B	0042867	biological_process	pyruvate catabolic process
B	0046554	molecular_function	L-malate dehydrogenase (NADP+) activity
B	0102443	molecular_function	L-2-hydroxycarboxylate dehydrogenase (NAD+) activity
C	0004090	molecular_function	carbonyl reductase (NADPH) activity
C	0004459	molecular_function	L-lactate dehydrogenase (NAD+) activity
C	0005737	cellular_component	cytoplasm
C	0006089	biological_process	lactate metabolic process
C	0030060	molecular_function	L-malate dehydrogenase (NAD+) activity
C	0042867	biological_process	pyruvate catabolic process
C	0046554	molecular_function	L-malate dehydrogenase (NADP+) activity
C	0102443	molecular_function	L-2-hydroxycarboxylate dehydrogenase (NAD+) activity
D	0004090	molecular_function	carbonyl reductase (NADPH) activity
D	0004459	molecular_function	L-lactate dehydrogenase (NAD+) activity
D	0005737	cellular_component	cytoplasm
D	0006089	biological_process	lactate metabolic process
D	0030060	molecular_function	L-malate dehydrogenase (NAD+) activity
D	0042867	biological_process	pyruvate catabolic process
D	0046554	molecular_function	L-malate dehydrogenase (NADP+) activity
D	0102443	molecular_function	L-2-hydroxycarboxylate dehydrogenase (NAD+) activity

Functional Information from PROSITE/UniProt

site_id	PS00064
Number of Residues	7
Details	L_LDH L-lactate dehydrogenase active site. IGEHGDS

Chain	Residue	Details
A	ILE175-SER181

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"UniProtKB","id":"P61889","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	20
Details	Binding site: {"evidences":[{"source":"PubMed","id":"11292347","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1HYG","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PDB","id":"1HYG","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	15
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"P61889","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	4
Details	Site: {"description":"Discrimination site allowing the use of NADPH","evidences":[{"source":"PubMed","id":"10998181","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	3
Details	Site: {"description":"Discrimination site conferring specificity for malate over lactate","evidences":[{"source":"PubMed","id":"10998181","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---