9OT6
Cryo-EM structure of the PI4KA complex bound to an EFR3 interfering nanobody (F3IN)
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0004430 | molecular_function | 1-phosphatidylinositol 4-kinase activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005524 | molecular_function | ATP binding |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0005925 | cellular_component | focal adhesion |
| A | 0006629 | biological_process | lipid metabolic process |
| A | 0006661 | biological_process | phosphatidylinositol biosynthetic process |
| A | 0007165 | biological_process | signal transduction |
| A | 0016020 | cellular_component | membrane |
| A | 0016301 | molecular_function | kinase activity |
| A | 0016740 | molecular_function | transferase activity |
| A | 0030660 | cellular_component | Golgi-associated vesicle membrane |
| A | 0044788 | biological_process | host-mediated perturbation of viral process |
| A | 0045296 | molecular_function | cadherin binding |
| A | 0046854 | biological_process | phosphatidylinositol phosphate biosynthetic process |
| A | 0048015 | biological_process | phosphatidylinositol-mediated signaling |
| A | 0070062 | cellular_component | extracellular exosome |
| A | 0140754 | biological_process | reorganization of cellular membranes to establish viral sites of replication |
| B | 0000166 | molecular_function | nucleotide binding |
| B | 0004430 | molecular_function | 1-phosphatidylinositol 4-kinase activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005524 | molecular_function | ATP binding |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005829 | cellular_component | cytosol |
| B | 0005886 | cellular_component | plasma membrane |
| B | 0005925 | cellular_component | focal adhesion |
| B | 0006629 | biological_process | lipid metabolic process |
| B | 0006661 | biological_process | phosphatidylinositol biosynthetic process |
| B | 0007165 | biological_process | signal transduction |
| B | 0016020 | cellular_component | membrane |
| B | 0016301 | molecular_function | kinase activity |
| B | 0016740 | molecular_function | transferase activity |
| B | 0030660 | cellular_component | Golgi-associated vesicle membrane |
| B | 0044788 | biological_process | host-mediated perturbation of viral process |
| B | 0045296 | molecular_function | cadherin binding |
| B | 0046854 | biological_process | phosphatidylinositol phosphate biosynthetic process |
| B | 0048015 | biological_process | phosphatidylinositol-mediated signaling |
| B | 0070062 | cellular_component | extracellular exosome |
| B | 0140754 | biological_process | reorganization of cellular membranes to establish viral sites of replication |
| D | 0005515 | molecular_function | protein binding |
| D | 0005829 | cellular_component | cytosol |
| D | 0005886 | cellular_component | plasma membrane |
| D | 0046854 | biological_process | phosphatidylinositol phosphate biosynthetic process |
| D | 0072659 | biological_process | protein localization to plasma membrane |
| F | 0005515 | molecular_function | protein binding |
| F | 0005829 | cellular_component | cytosol |
| F | 0005886 | cellular_component | plasma membrane |
| F | 0046854 | biological_process | phosphatidylinositol phosphate biosynthetic process |
| F | 0072659 | biological_process | protein localization to plasma membrane |
Functional Information from PROSITE/UniProt
| site_id | PS00063 |
| Number of Residues | 16 |
| Details | ALDOKETO_REDUCTASE_3 Aldo/keto reductase family putative active site signature. LARSLAvqRPaSLeKV |
| Chain | Residue | Details |
| A | LEU38-VAL53 |
| site_id | PS00915 |
| Number of Residues | 15 |
| Details | PI3_4_KINASE_1 Phosphatidylinositol 3- and 4-kinases signature 1. FKvg.DDCRQDmlalQ |
| Chain | Residue | Details |
| A | PHE1849-GLN1863 |
| site_id | PS00916 |
| Number of Residues | 21 |
| Details | PI3_4_KINASE_2 Phosphatidylinositol 3- and 4-kinases signature 2. SmAaysLllFLLqIkDRHngN |
| Chain | Residue | Details |
| A | SER1942-ASN1962 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 376 |
| Details | Domain: {"description":"PIK helical","evidences":[{"source":"PROSITE-ProRule","id":"PRU00878","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 12 |
| Details | Region: {"description":"G-loop","evidences":[{"source":"PROSITE-ProRule","id":"PRU00269","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 16 |
| Details | Region: {"description":"Catalytic loop","evidences":[{"source":"PROSITE-ProRule","id":"PRU00269","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 48 |
| Details | Region: {"description":"Activation loop","evidences":[{"source":"PROSITE-ProRule","id":"PRU00269","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"Phosphotyrosine","evidences":[{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 66 |
| Details | Repeat: {"description":"TPR 2"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 66 |
| Details | Repeat: {"description":"TPR 3"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 66 |
| Details | Repeat: {"description":"TPR 4"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI9 |
| Number of Residues | 70 |
| Details | Repeat: {"description":"TPR 5"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI10 |
| Number of Residues | 64 |
| Details | Repeat: {"description":"TPR 6"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI11 |
| Number of Residues | 66 |
| Details | Repeat: {"description":"TPR 7"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI12 |
| Number of Residues | 66 |
| Details | Repeat: {"description":"TPR 8"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI13 |
| Number of Residues | 66 |
| Details | Repeat: {"description":"TPR 9"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI14 |
| Number of Residues | 64 |
| Details | Repeat: {"description":"TPR 10"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI15 |
| Number of Residues | 66 |
| Details | Repeat: {"description":"TPR 11"} |
| Chain | Residue | Details |
