9M1M
Cryo-EM structure of the TBC-DEC-Arl2-alpha-beta-tubulin complex with GDP-AlFx
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| a | 0000166 | molecular_function | nucleotide binding |
| a | 0000226 | biological_process | microtubule cytoskeleton organization |
| a | 0000278 | biological_process | mitotic cell cycle |
| a | 0003924 | molecular_function | GTPase activity |
| a | 0005200 | molecular_function | structural constituent of cytoskeleton |
| a | 0005525 | molecular_function | GTP binding |
| a | 0005737 | cellular_component | cytoplasm |
| a | 0005856 | cellular_component | cytoskeleton |
| a | 0005874 | cellular_component | microtubule |
| a | 0015630 | cellular_component | microtubule cytoskeleton |
| a | 0016787 | molecular_function | hydrolase activity |
| a | 0030182 | biological_process | neuron differentiation |
| a | 0046872 | molecular_function | metal ion binding |
| b | 0000166 | molecular_function | nucleotide binding |
| b | 0000226 | biological_process | microtubule cytoskeleton organization |
| b | 0000278 | biological_process | mitotic cell cycle |
| b | 0005200 | molecular_function | structural constituent of cytoskeleton |
| b | 0005515 | molecular_function | protein binding |
| b | 0005525 | molecular_function | GTP binding |
| b | 0005634 | cellular_component | nucleus |
| b | 0005641 | cellular_component | nuclear envelope lumen |
| b | 0005737 | cellular_component | cytoplasm |
| b | 0005856 | cellular_component | cytoskeleton |
| b | 0005874 | cellular_component | microtubule |
| b | 0005929 | cellular_component | cilium |
| b | 0015630 | cellular_component | microtubule cytoskeleton |
| b | 0031625 | molecular_function | ubiquitin protein ligase binding |
| b | 0036464 | cellular_component | cytoplasmic ribonucleoprotein granule |
| b | 0042288 | molecular_function | MHC class I protein binding |
| b | 0044297 | cellular_component | cell body |
| b | 0045171 | cellular_component | intercellular bridge |
| b | 0046872 | molecular_function | metal ion binding |
| b | 0050807 | biological_process | regulation of synapse organization |
| b | 0072686 | cellular_component | mitotic spindle |
| b | 0097228 | cellular_component | sperm principal piece |
| b | 0097229 | cellular_component | sperm end piece |
| b | 0097542 | cellular_component | ciliary tip |
| C | 0003924 | molecular_function | GTPase activity |
| C | 0005096 | molecular_function | GTPase activator activity |
| C | 0005515 | molecular_function | protein binding |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005829 | cellular_component | cytosol |
| C | 0005856 | cellular_component | cytoskeleton |
| C | 0005874 | cellular_component | microtubule |
| C | 0006457 | biological_process | protein folding |
| C | 0007021 | biological_process | tubulin complex assembly |
| C | 0007023 | biological_process | post-chaperonin tubulin folding pathway |
| C | 0032391 | cellular_component | photoreceptor connecting cilium |
| C | 0051087 | molecular_function | protein-folding chaperone binding |
| D | 0000226 | biological_process | microtubule cytoskeleton organization |
| D | 0000278 | biological_process | mitotic cell cycle |
| D | 0005096 | molecular_function | GTPase activator activity |
| D | 0005515 | molecular_function | protein binding |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0005813 | cellular_component | centrosome |
| D | 0005874 | cellular_component | microtubule |
| D | 0005912 | cellular_component | adherens junction |
| D | 0005923 | cellular_component | bicellular tight junction |
| D | 0006457 | biological_process | protein folding |
| D | 0007021 | biological_process | tubulin complex assembly |
| D | 0007023 | biological_process | post-chaperonin tubulin folding pathway |
| D | 0010812 | biological_process | negative regulation of cell-substrate adhesion |
| D | 0016328 | cellular_component | lateral plasma membrane |
| D | 0031115 | biological_process | negative regulation of microtubule polymerization |
| D | 0034333 | biological_process | adherens junction assembly |
| D | 0048487 | molecular_function | beta-tubulin binding |
| D | 0048667 | biological_process | cell morphogenesis involved in neuron differentiation |
| D | 0051087 | molecular_function | protein-folding chaperone binding |
| D | 0070830 | biological_process | bicellular tight junction assembly |
| E | 0000226 | biological_process | microtubule cytoskeleton organization |
| E | 0005515 | molecular_function | protein binding |
| E | 0005737 | cellular_component | cytoplasm |
| E | 0005856 | cellular_component | cytoskeleton |
| E | 0005874 | cellular_component | microtubule |
| E | 0006457 | biological_process | protein folding |
| E | 0007021 | biological_process | tubulin complex assembly |
| E | 0007023 | biological_process | post-chaperonin tubulin folding pathway |
| E | 0007052 | biological_process | mitotic spindle organization |
| E | 0043014 | molecular_function | alpha-tubulin binding |
| E | 0051087 | molecular_function | protein-folding chaperone binding |
| G | 0000166 | molecular_function | nucleotide binding |
| G | 0003924 | molecular_function | GTPase activity |
| G | 0005515 | molecular_function | protein binding |
| G | 0005525 | molecular_function | GTP binding |
| G | 0005634 | cellular_component | nucleus |
| G | 0005654 | cellular_component | nucleoplasm |
| G | 0005730 | cellular_component | nucleolus |
| G | 0005737 | cellular_component | cytoplasm |
| G | 0005739 | cellular_component | mitochondrion |
| G | 0005758 | cellular_component | mitochondrial intermembrane space |
| G | 0005759 | cellular_component | mitochondrial matrix |
| G | 0005813 | cellular_component | centrosome |
| G | 0005829 | cellular_component | cytosol |
| G | 0005929 | cellular_component | cilium |
| G | 0006110 | biological_process | regulation of glycolytic process |
| G | 0006457 | biological_process | protein folding |
| G | 0007098 | biological_process | centrosome cycle |
| G | 0010811 | biological_process | positive regulation of cell-substrate adhesion |
| G | 0015630 | cellular_component | microtubule cytoskeleton |
| G | 0016328 | cellular_component | lateral plasma membrane |
| G | 0019003 | molecular_function | GDP binding |
| G | 0031113 | biological_process | regulation of microtubule polymerization |
| G | 0031116 | biological_process | positive regulation of microtubule polymerization |
| G | 0034260 | biological_process | negative regulation of GTPase activity |
| G | 0051457 | biological_process | maintenance of protein location in nucleus |
| G | 0070830 | biological_process | bicellular tight junction assembly |
| G | 1903715 | biological_process | regulation of aerobic respiration |
Functional Information from PROSITE/UniProt
| site_id | PS00227 |
| Number of Residues | 7 |
| Details | TUBULIN Tubulin subunits alpha, beta, and gamma signature. GGGTGSG |
| Chain | Residue | Details |
| b | GLY140-GLY146 | |
| a | GLY142-GLY148 |
| site_id | PS00228 |
| Number of Residues | 4 |
| Details | TUBULIN_B_AUTOREG Tubulin-beta mRNA autoregulation signal. MREI |
| Chain | Residue | Details |
| b | MET1-ILE4 |
| site_id | PS00845 |
| Number of Residues | 33 |
| Details | CAP_GLY_1 CAP-Gly domain signature. Gvvppvagp..WlGVewDnper...GKHdGSheGtvYF |
| Chain | Residue | Details |
| E | GLY27-PHE59 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 38 |
| Details | Repeat: {"description":"HEAT 1"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 37 |
| Details | Repeat: {"description":"HEAT 2"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 36 |
| Details | Repeat: {"description":"HEAT 3"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 44 |
| Details | Domain: {"description":"CAP-Gly","evidences":[{"source":"PROSITE-ProRule","id":"PRU00045","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 21 |
| Details | Repeat: {"description":"LRR 1"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 20 |
| Details | Repeat: {"description":"LRR 2"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 21 |
| Details | Repeat: {"description":"LRR 3"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 22 |
| Details | Repeat: {"description":"LRR 4"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI9 |
| Number of Residues | 21 |
| Details | Repeat: {"description":"LRR 5"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI10 |
| Number of Residues | 21 |
| Details | Repeat: {"description":"LRR 6"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI11 |
| Number of Residues | 21 |
| Details | Repeat: {"description":"LRR 7"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI12 |
| Number of Residues | 42 |
| Details | Domain: {"description":"LRRCT"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI13 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"N-acetylserine","evidences":[{"source":"PubMed","id":"19413330","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"22223895","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"22814378","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI14 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"19608861","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI15 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"18669648","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI16 |
| Number of Residues | 15 |
| Details | Binding site: {"evidences":[{"evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI17 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"Q9D0J4","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI18 |
| Number of Residues | 1 |
| Details | Lipidation: {"description":"N-myristoyl glycine","evidences":[{"evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI19 |
| Number of Residues | 2 |
| Details | Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI20 |
| Number of Residues | 3 |
| Details | Motif: {"description":"MREC motif","evidences":[{"source":"UniProtKB","id":"P68363","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI21 |
| Number of Residues | 1 |
| Details | Active site: {"evidences":[{"source":"UniProtKB","id":"P68363","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI22 |
| Number of Residues | 18 |
| Details | Binding site: {"evidences":[{"source":"UniProtKB","id":"P68363","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI23 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"N6-acetyllysine; alternate","evidences":[{"source":"UniProtKB","id":"P68363","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI24 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"P68363","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI25 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"3'-nitrotyrosine","evidences":[{"source":"UniProtKB","id":"P68373","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI26 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"Omega-N-methylarginine","evidences":[{"source":"UniProtKB","id":"P68363","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI27 |
| Number of Residues | 3 |
| Details | Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"UniProtKB","id":"P68363","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI28 |
| Number of Residues | 3 |
| Details | Motif: {"description":"MREI motif","evidences":[{"source":"UniProtKB","id":"P07437","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI29 |
| Number of Residues | 7 |
| Details | Binding site: {"evidences":[{"source":"UniProtKB","id":"Q13509","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI30 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"P99024","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI31 |
| Number of Residues | 3 |
| Details | Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"UniProtKB","id":"P07437","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI32 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"N6-succinyllysine; alternate","evidences":[{"source":"UniProtKB","id":"P99024","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI33 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"Phosphoserine; by CDK1","evidences":[{"source":"UniProtKB","id":"P07437","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI34 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"Omega-N-methylarginine","evidences":[{"source":"UniProtKB","id":"P07437","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI35 |
| Number of Residues | 1 |
| Details | Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate","evidences":[{"source":"UniProtKB","id":"P07437","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI36 |
| Number of Residues | 2 |
| Details | Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"UniProtKB","id":"P07437","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
