9L5R

Cryo-EM structure of the thermophile spliceosome (state ILS)

This is a non-PDB format compatible entry.

Functional Information from GO Data

Chain	GOid	namespace	contents
0	0000974	cellular_component	Prp19 complex
0	0003723	molecular_function	RNA binding
0	0005634	cellular_component	nucleus
0	0017070	molecular_function	U6 snRNA binding
0	0036002	molecular_function	pre-mRNA binding
0	0071006	cellular_component	U2-type catalytic step 1 spliceosome
0	0071007	cellular_component	U2-type catalytic step 2 spliceosome
a	0000387	biological_process	spliceosomal snRNP assembly
a	0000398	biological_process	mRNA splicing, via spliceosome
a	0003723	molecular_function	RNA binding
a	0005634	cellular_component	nucleus
a	0005681	cellular_component	spliceosomal complex
a	0005682	cellular_component	U5 snRNP
a	0005685	cellular_component	U1 snRNP
a	0005686	cellular_component	U2 snRNP
a	0005687	cellular_component	U4 snRNP
a	0005737	cellular_component	cytoplasm
a	0032991	cellular_component	protein-containing complex
a	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
A	0000398	biological_process	mRNA splicing, via spliceosome
A	0003723	molecular_function	RNA binding
A	0005681	cellular_component	spliceosomal complex
A	0008233	molecular_function	peptidase activity
A	0008237	molecular_function	metallopeptidase activity
A	0017070	molecular_function	U6 snRNA binding
A	0030623	molecular_function	U5 snRNA binding
b	0000398	biological_process	mRNA splicing, via spliceosome
b	0003723	molecular_function	RNA binding
b	0005634	cellular_component	nucleus
b	0005681	cellular_component	spliceosomal complex
b	0005685	cellular_component	U1 snRNP
b	0034715	cellular_component	pICln-Sm protein complex
b	0071013	cellular_component	catalytic step 2 spliceosome
b	0120114	cellular_component	Sm-like protein family complex
B	0000398	biological_process	mRNA splicing, via spliceosome
B	0000974	cellular_component	Prp19 complex
B	0005634	cellular_component	nucleus
B	0006397	biological_process	mRNA processing
B	0008380	biological_process	RNA splicing
B	0071013	cellular_component	catalytic step 2 spliceosome
B	0071014	cellular_component	post-mRNA release spliceosomal complex
c	0000398	biological_process	mRNA splicing, via spliceosome
c	0000974	cellular_component	Prp19 complex
c	0003723	molecular_function	RNA binding
c	0005634	cellular_component	nucleus
c	0005681	cellular_component	spliceosomal complex
c	0005682	cellular_component	U5 snRNP
c	0005685	cellular_component	U1 snRNP
c	0005789	cellular_component	endoplasmic reticulum membrane
c	0005829	cellular_component	cytosol
c	0006696	biological_process	ergosterol biosynthetic process
c	0008380	biological_process	RNA splicing
c	0016020	cellular_component	membrane
c	0016126	biological_process	sterol biosynthetic process
c	0016627	molecular_function	oxidoreductase activity, acting on the CH-CH group of donors
c	0016628	molecular_function	oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor
c	0030532	cellular_component	small nuclear ribonucleoprotein complex
c	0050613	molecular_function	Delta14-sterol reductase activity
C	0003924	molecular_function	GTPase activity
C	0005525	molecular_function	GTP binding
Cb	0000390	biological_process	spliceosomal complex disassembly
Cb	0000398	biological_process	mRNA splicing, via spliceosome
Cb	0003676	molecular_function	nucleic acid binding
Cb	0005634	cellular_component	nucleus
Cb	0071008	cellular_component	U2-type post-mRNA release spliceosomal complex
Cc	0000390	biological_process	spliceosomal complex disassembly
Cc	0003676	molecular_function	nucleic acid binding
Cc	0003723	molecular_function	RNA binding
Cc	0003724	molecular_function	RNA helicase activity
Cc	0004386	molecular_function	helicase activity
Cc	0005524	molecular_function	ATP binding
Cc	0005634	cellular_component	nucleus
Cc	0005681	cellular_component	spliceosomal complex
Cc	0071014	cellular_component	post-mRNA release spliceosomal complex
Ci	0003676	molecular_function	nucleic acid binding
Ci	0003723	molecular_function	RNA binding
CY	0000390	biological_process	spliceosomal complex disassembly
CY	0071008	cellular_component	U2-type post-mRNA release spliceosomal complex
d	0000387	biological_process	spliceosomal snRNP assembly
d	0003723	molecular_function	RNA binding
d	0005634	cellular_component	nucleus
d	0006396	biological_process	RNA processing
d	0010468	biological_process	regulation of gene expression
d	0031981	cellular_component	nuclear lumen
d	0097525	cellular_component	spliceosomal snRNP complex
D	0005634	cellular_component	nucleus
D	0005737	cellular_component	cytoplasm
e	0000398	biological_process	mRNA splicing, via spliceosome
e	0003723	molecular_function	RNA binding
e	0005634	cellular_component	nucleus
e	0005682	cellular_component	U5 snRNP
e	0005685	cellular_component	U1 snRNP
e	0005686	cellular_component	U2 snRNP
e	0005687	cellular_component	U4 snRNP
e	0005737	cellular_component	cytoplasm
e	0032991	cellular_component	protein-containing complex
e	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
e	0070990	molecular_function	snRNP binding
e	0071004	cellular_component	U2-type prespliceosome
e	0071013	cellular_component	catalytic step 2 spliceosome
E	0003723	molecular_function	RNA binding
E	0071013	cellular_component	catalytic step 2 spliceosome
f	0000387	biological_process	spliceosomal snRNP assembly
f	0003723	molecular_function	RNA binding
f	0005634	cellular_component	nucleus
f	0005681	cellular_component	spliceosomal complex
f	0005685	cellular_component	U1 snRNP
f	0005829	cellular_component	cytosol
f	0006396	biological_process	RNA processing
F	0005684	cellular_component	U2-type spliceosomal complex
F	0071014	cellular_component	post-mRNA release spliceosomal complex
g	0000387	biological_process	spliceosomal snRNP assembly
g	0000398	biological_process	mRNA splicing, via spliceosome
g	0003723	molecular_function	RNA binding
g	0005634	cellular_component	nucleus
g	0005681	cellular_component	spliceosomal complex
g	0005682	cellular_component	U5 snRNP
g	0005685	cellular_component	U1 snRNP
g	0005686	cellular_component	U2 snRNP
g	0005687	cellular_component	U4 snRNP
g	0032991	cellular_component	protein-containing complex
g	0034719	cellular_component	SMN-Sm protein complex
g	0071004	cellular_component	U2-type prespliceosome
g	0071011	cellular_component	precatalytic spliceosome
g	0071013	cellular_component	catalytic step 2 spliceosome
g	0097526	cellular_component	spliceosomal tri-snRNP complex
g	1990904	cellular_component	ribonucleoprotein complex
h	0000398	biological_process	mRNA splicing, via spliceosome
h	0005634	cellular_component	nucleus
h	0005686	cellular_component	U2 snRNP
h	0030620	molecular_function	U2 snRNA binding
i	0003676	molecular_function	nucleic acid binding
i	0003723	molecular_function	RNA binding
I	0000349	biological_process	generation of catalytic spliceosome for first transesterification step
I	0000398	biological_process	mRNA splicing, via spliceosome
I	0000974	cellular_component	Prp19 complex
I	0005634	cellular_component	nucleus
I	0071007	cellular_component	U2-type catalytic step 2 spliceosome
I	0071014	cellular_component	post-mRNA release spliceosomal complex
j	0000387	biological_process	spliceosomal snRNP assembly
j	0000398	biological_process	mRNA splicing, via spliceosome
j	0003723	molecular_function	RNA binding
j	0005634	cellular_component	nucleus
j	0005681	cellular_component	spliceosomal complex
j	0005682	cellular_component	U5 snRNP
j	0005685	cellular_component	U1 snRNP
j	0005686	cellular_component	U2 snRNP
j	0005687	cellular_component	U4 snRNP
j	0005737	cellular_component	cytoplasm
j	0032991	cellular_component	protein-containing complex
j	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
J	0000245	biological_process	spliceosomal complex assembly
J	0000398	biological_process	mRNA splicing, via spliceosome
J	0000974	cellular_component	Prp19 complex
J	0005634	cellular_component	nucleus
J	0071007	cellular_component	U2-type catalytic step 2 spliceosome
J	0071011	cellular_component	precatalytic spliceosome
J	0071014	cellular_component	post-mRNA release spliceosomal complex
k	0000387	biological_process	spliceosomal snRNP assembly
k	0000398	biological_process	mRNA splicing, via spliceosome
k	0003723	molecular_function	RNA binding
k	0005634	cellular_component	nucleus
k	0005681	cellular_component	spliceosomal complex
k	0005682	cellular_component	U5 snRNP
k	0005685	cellular_component	U1 snRNP
k	0005686	cellular_component	U2 snRNP
k	0005687	cellular_component	U4 snRNP
k	0032991	cellular_component	protein-containing complex
k	0034719	cellular_component	SMN-Sm protein complex
k	0071004	cellular_component	U2-type prespliceosome
k	0071011	cellular_component	precatalytic spliceosome
k	0071013	cellular_component	catalytic step 2 spliceosome
k	0097526	cellular_component	spliceosomal tri-snRNP complex
k	1990904	cellular_component	ribonucleoprotein complex
K	0000974	cellular_component	Prp19 complex
K	0005634	cellular_component	nucleus
K	0071011	cellular_component	precatalytic spliceosome
K	0071013	cellular_component	catalytic step 2 spliceosome
l	0000398	biological_process	mRNA splicing, via spliceosome
l	0003723	molecular_function	RNA binding
l	0005634	cellular_component	nucleus
l	0005681	cellular_component	spliceosomal complex
l	0005685	cellular_component	U1 snRNP
l	0034715	cellular_component	pICln-Sm protein complex
l	0071013	cellular_component	catalytic step 2 spliceosome
l	0120114	cellular_component	Sm-like protein family complex
L	0000398	biological_process	mRNA splicing, via spliceosome
L	0000974	cellular_component	Prp19 complex
L	0005681	cellular_component	spliceosomal complex
m	0000398	biological_process	mRNA splicing, via spliceosome
m	0000974	cellular_component	Prp19 complex
m	0003723	molecular_function	RNA binding
m	0005634	cellular_component	nucleus
m	0005681	cellular_component	spliceosomal complex
m	0005682	cellular_component	U5 snRNP
m	0005685	cellular_component	U1 snRNP
m	0005789	cellular_component	endoplasmic reticulum membrane
m	0005829	cellular_component	cytosol
m	0006696	biological_process	ergosterol biosynthetic process
m	0008380	biological_process	RNA splicing
m	0016020	cellular_component	membrane
m	0016126	biological_process	sterol biosynthetic process
m	0016627	molecular_function	oxidoreductase activity, acting on the CH-CH group of donors
m	0016628	molecular_function	oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor
m	0030532	cellular_component	small nuclear ribonucleoprotein complex
m	0050613	molecular_function	Delta14-sterol reductase activity
M	0000974	cellular_component	Prp19 complex
M	0003723	molecular_function	RNA binding
M	0005634	cellular_component	nucleus
M	0017070	molecular_function	U6 snRNA binding
M	0036002	molecular_function	pre-mRNA binding
M	0071006	cellular_component	U2-type catalytic step 1 spliceosome
M	0071007	cellular_component	U2-type catalytic step 2 spliceosome
N	0000398	biological_process	mRNA splicing, via spliceosome
N	0005634	cellular_component	nucleus
N	0005681	cellular_component	spliceosomal complex
o	0000387	biological_process	spliceosomal snRNP assembly
o	0003723	molecular_function	RNA binding
o	0005634	cellular_component	nucleus
o	0006396	biological_process	RNA processing
o	0010468	biological_process	regulation of gene expression
o	0031981	cellular_component	nuclear lumen
o	0097525	cellular_component	spliceosomal snRNP complex
p	0000398	biological_process	mRNA splicing, via spliceosome
p	0003723	molecular_function	RNA binding
p	0005634	cellular_component	nucleus
p	0005682	cellular_component	U5 snRNP
p	0005685	cellular_component	U1 snRNP
p	0005686	cellular_component	U2 snRNP
p	0005687	cellular_component	U4 snRNP
p	0005737	cellular_component	cytoplasm
p	0032991	cellular_component	protein-containing complex
p	0046540	cellular_component	U4/U6 x U5 tri-snRNP complex
p	0070990	molecular_function	snRNP binding
p	0071004	cellular_component	U2-type prespliceosome
p	0071013	cellular_component	catalytic step 2 spliceosome
P	0000398	biological_process	mRNA splicing, via spliceosome
P	0003723	molecular_function	RNA binding
P	0005681	cellular_component	spliceosomal complex
P	0045292	biological_process	mRNA cis splicing, via spliceosome
P	0071013	cellular_component	catalytic step 2 spliceosome
q	0000398	biological_process	mRNA splicing, via spliceosome
q	0000974	cellular_component	Prp19 complex
q	0004842	molecular_function	ubiquitin-protein transferase activity
q	0005634	cellular_component	nucleus
q	0005737	cellular_component	cytoplasm
q	0016567	biological_process	protein ubiquitination
q	0061630	molecular_function	ubiquitin protein ligase activity
q	0070534	biological_process	protein K63-linked ubiquitination
q	0071006	cellular_component	U2-type catalytic step 1 spliceosome
r	0000398	biological_process	mRNA splicing, via spliceosome
r	0000974	cellular_component	Prp19 complex
r	0004842	molecular_function	ubiquitin-protein transferase activity
r	0005634	cellular_component	nucleus
r	0005737	cellular_component	cytoplasm
r	0016567	biological_process	protein ubiquitination
r	0061630	molecular_function	ubiquitin protein ligase activity
r	0070534	biological_process	protein K63-linked ubiquitination
r	0071006	cellular_component	U2-type catalytic step 1 spliceosome
R	0000398	biological_process	mRNA splicing, via spliceosome
R	0005634	cellular_component	nucleus
R	0005681	cellular_component	spliceosomal complex
s	0000398	biological_process	mRNA splicing, via spliceosome
s	0000974	cellular_component	Prp19 complex
s	0004842	molecular_function	ubiquitin-protein transferase activity
s	0005634	cellular_component	nucleus
s	0005737	cellular_component	cytoplasm
s	0016567	biological_process	protein ubiquitination
s	0061630	molecular_function	ubiquitin protein ligase activity
s	0070534	biological_process	protein K63-linked ubiquitination
s	0071006	cellular_component	U2-type catalytic step 1 spliceosome
S	0003755	molecular_function	peptidyl-prolyl cis-trans isomerase activity
S	0005634	cellular_component	nucleus
S	0071013	cellular_component	catalytic step 2 spliceosome
t	0000398	biological_process	mRNA splicing, via spliceosome
t	0000974	cellular_component	Prp19 complex
t	0004842	molecular_function	ubiquitin-protein transferase activity
t	0005634	cellular_component	nucleus
t	0005737	cellular_component	cytoplasm
t	0016567	biological_process	protein ubiquitination
t	0061630	molecular_function	ubiquitin protein ligase activity
t	0070534	biological_process	protein K63-linked ubiquitination
t	0071006	cellular_component	U2-type catalytic step 1 spliceosome
T	0000398	biological_process	mRNA splicing, via spliceosome
T	0000974	cellular_component	Prp19 complex
T	0005634	cellular_component	nucleus
T	0071011	cellular_component	precatalytic spliceosome
T	0071013	cellular_component	catalytic step 2 spliceosome
u	0000387	biological_process	spliceosomal snRNP assembly
u	0003723	molecular_function	RNA binding
u	0005634	cellular_component	nucleus
u	0005681	cellular_component	spliceosomal complex
u	0005685	cellular_component	U1 snRNP
u	0005829	cellular_component	cytosol
u	0006396	biological_process	RNA processing
U	0000398	biological_process	mRNA splicing, via spliceosome
U	0071014	cellular_component	post-mRNA release spliceosomal complex
W	0000398	biological_process	mRNA splicing, via spliceosome
W	0003729	molecular_function	mRNA binding
W	0071013	cellular_component	catalytic step 2 spliceosome
Y	0000398	biological_process	mRNA splicing, via spliceosome
Y	0003729	molecular_function	mRNA binding
Y	0004386	molecular_function	helicase activity
Y	0005634	cellular_component	nucleus
Y	0005681	cellular_component	spliceosomal complex
Y	0005684	cellular_component	U2-type spliceosomal complex
Y	0045292	biological_process	mRNA cis splicing, via spliceosome
Y	0071013	cellular_component	catalytic step 2 spliceosome

Functional Information from PROSITE/UniProt

site_id	PS00131
Number of Residues	8
Details	CARBOXYPEPT_SER_SER Serine carboxypeptidases, serine active site. MySESYGG

Chain	Residue	Details
C	MET370-GLY377

---

site_id	PS00170
Number of Residues	18
Details	CSA_PPIASE_1 Cyclophilin-type peptidyl-prolyl cis-trans isomerase signature. YdgTiFHRVIkdFMvQGG

Chain	Residue	Details
S	TYR39-GLY56

---

site_id	PS00430
Number of Residues	63
Details	TONB_DEPENDENT_REC_1 TonB-dependent receptor (TBDR) proteins signature 1. mklvrflmkcanetvtielkngaivhgtiasvtpkmdtalrnvrytpkgeepysv............................................................ESLTVRGN

Chain	Residue	Details
d	MET1-ASN63

---

site_id	PS00678
Number of Residues	15
Details	WD_REPEATS_1 Trp-Asp (WD) repeats signature. LVTGgrDgVARVWDM

Chain	Residue	Details
T	LEU286-MET300
T	VAL328-LEU342
E	LEU118-LEU132
E	ILE202-ILE216

---

site_id	PS00690
Number of Residues	10
Details	DEAH_ATP_HELICASE DEAH-box subfamily ATP-dependent helicases signature. ScIILDEAHE

Chain	Residue	Details
Cc	SER213-GLU222

---

site_id	PS00997
Number of Residues	23
Details	G10_1 G10 protein signature 1. LCClRCiqtkEtNfnstCiCRVP

Chain	Residue	Details
N	LEU102-PRO124

---

site_id	PS01017
Number of Residues	16
Details	STEROL_REDUCT_1 Sterol reductase family signature 1. GniLYDwFiGreLNPR

Chain	Residue	Details
c	GLY-286-ARG-271

---

site_id	PS01018
Number of Residues	24
Details	STEROL_REDUCT_2 Sterol reductase family signature 2. LLLHRdrRDdekCskKYGedWekY

Chain	Residue	Details
c	LEU-33-TYR-10

---