9IQX

Cryo-EM structure of the human TRPV4-RhoA in complex with AH001

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005216	molecular_function	monoatomic ion channel activity
A	0006811	biological_process	monoatomic ion transport
A	0016020	cellular_component	membrane
A	0055085	biological_process	transmembrane transport
B	0005216	molecular_function	monoatomic ion channel activity
B	0006811	biological_process	monoatomic ion transport
B	0016020	cellular_component	membrane
B	0055085	biological_process	transmembrane transport
C	0005216	molecular_function	monoatomic ion channel activity
C	0006811	biological_process	monoatomic ion transport
C	0016020	cellular_component	membrane
C	0055085	biological_process	transmembrane transport
D	0005216	molecular_function	monoatomic ion channel activity
D	0006811	biological_process	monoatomic ion transport
D	0016020	cellular_component	membrane
D	0055085	biological_process	transmembrane transport
E	0000166	molecular_function	nucleotide binding
E	0000281	biological_process	mitotic cytokinesis
E	0002363	biological_process	alpha-beta T cell lineage commitment
E	0003189	biological_process	aortic valve formation
E	0003924	molecular_function	GTPase activity
E	0003925	molecular_function	G protein activity
E	0005515	molecular_function	protein binding
E	0005525	molecular_function	GTP binding
E	0005634	cellular_component	nucleus
E	0005737	cellular_component	cytoplasm
E	0005768	cellular_component	endosome
E	0005789	cellular_component	endoplasmic reticulum membrane
E	0005829	cellular_component	cytosol
E	0005856	cellular_component	cytoskeleton
E	0005886	cellular_component	plasma membrane
E	0005925	cellular_component	focal adhesion
E	0005938	cellular_component	cell cortex
E	0007264	biological_process	small GTPase-mediated signal transduction
E	0007266	biological_process	Rho protein signal transduction
E	0009898	cellular_component	cytoplasmic side of plasma membrane
E	0010812	biological_process	negative regulation of cell-substrate adhesion
E	0016477	biological_process	cell migration
E	0016787	molecular_function	hydrolase activity
E	0017022	molecular_function	myosin binding
E	0019901	molecular_function	protein kinase binding
E	0021762	biological_process	substantia nigra development
E	0030027	cellular_component	lamellipodium
E	0030036	biological_process	actin cytoskeleton organization
E	0030054	cellular_component	cell junction
E	0030334	biological_process	regulation of cell migration
E	0030335	biological_process	positive regulation of cell migration
E	0030425	cellular_component	dendrite
E	0030496	cellular_component	midbody
E	0030667	cellular_component	secretory granule membrane
E	0031122	biological_process	cytoplasmic microtubule organization
E	0031982	cellular_component	vesicle
E	0032154	cellular_component	cleavage furrow
E	0032467	biological_process	positive regulation of cytokinesis
E	0032587	cellular_component	ruffle membrane
E	0032956	biological_process	regulation of actin cytoskeleton organization
E	0033688	biological_process	regulation of osteoblast proliferation
E	0034329	biological_process	cell junction assembly
E	0034446	biological_process	substrate adhesion-dependent cell spreading
E	0035385	biological_process	Roundabout signaling pathway
E	0036089	biological_process	cleavage furrow formation
E	0038027	biological_process	apolipoprotein A-I-mediated signaling pathway
E	0042476	biological_process	odontogenesis
E	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
E	0043149	biological_process	stress fiber assembly
E	0043197	cellular_component	dendritic spine
E	0043296	cellular_component	apical junction complex
E	0043297	biological_process	apical junction assembly
E	0043366	biological_process	beta selection
E	0043542	biological_process	endothelial cell migration
E	0043931	biological_process	ossification involved in bone maturation
E	0044319	biological_process	wound healing, spreading of cells
E	0045198	biological_process	establishment of epithelial cell apical/basal polarity
E	0045666	biological_process	positive regulation of neuron differentiation
E	0045792	biological_process	negative regulation of cell size
E	0046638	biological_process	positive regulation of alpha-beta T cell differentiation
E	0050919	biological_process	negative chemotaxis
E	0051301	biological_process	cell division
E	0051496	biological_process	positive regulation of stress fiber assembly
E	0051893	biological_process	regulation of focal adhesion assembly
E	0060071	biological_process	Wnt signaling pathway, planar cell polarity pathway
E	0060193	biological_process	positive regulation of lipase activity
E	0061430	biological_process	bone trabecula morphogenesis
E	0070062	cellular_component	extracellular exosome
E	0070507	biological_process	regulation of microtubule cytoskeleton organization
E	0071222	biological_process	cellular response to lipopolysaccharide
E	0071345	biological_process	cellular response to cytokine stimulus
E	0071526	biological_process	semaphorin-plexin signaling pathway
E	0071902	biological_process	positive regulation of protein serine/threonine kinase activity
E	0071944	cellular_component	cell periphery
E	0090051	biological_process	negative regulation of cell migration involved in sprouting angiogenesis
E	0090307	biological_process	mitotic spindle assembly
E	0090324	biological_process	negative regulation of oxidative phosphorylation
E	0097498	biological_process	endothelial tube lumen extension
E	0098794	cellular_component	postsynapse
E	0098978	cellular_component	glutamatergic synapse
E	0099159	biological_process	regulation of modification of postsynaptic structure
E	0101003	cellular_component	ficolin-1-rich granule membrane
E	1901224	biological_process	positive regulation of non-canonical NF-kappaB signal transduction
E	1902766	biological_process	skeletal muscle satellite cell migration
E	1903427	biological_process	negative regulation of reactive oxygen species biosynthetic process
E	1903673	biological_process	mitotic cleavage furrow formation
E	1904996	biological_process	positive regulation of leukocyte adhesion to vascular endothelial cell
E	1905274	biological_process	regulation of modification of postsynaptic actin cytoskeleton
E	1990869	biological_process	cellular response to chemokine
E	2000406	biological_process	positive regulation of T cell migration
F	0000166	molecular_function	nucleotide binding
F	0000281	biological_process	mitotic cytokinesis
F	0002363	biological_process	alpha-beta T cell lineage commitment
F	0003189	biological_process	aortic valve formation
F	0003924	molecular_function	GTPase activity
F	0003925	molecular_function	G protein activity
F	0005515	molecular_function	protein binding
F	0005525	molecular_function	GTP binding
F	0005634	cellular_component	nucleus
F	0005737	cellular_component	cytoplasm
F	0005768	cellular_component	endosome
F	0005789	cellular_component	endoplasmic reticulum membrane
F	0005829	cellular_component	cytosol
F	0005856	cellular_component	cytoskeleton
F	0005886	cellular_component	plasma membrane
F	0005925	cellular_component	focal adhesion
F	0005938	cellular_component	cell cortex
F	0007264	biological_process	small GTPase-mediated signal transduction
F	0007266	biological_process	Rho protein signal transduction
F	0009898	cellular_component	cytoplasmic side of plasma membrane
F	0010812	biological_process	negative regulation of cell-substrate adhesion
F	0016477	biological_process	cell migration
F	0016787	molecular_function	hydrolase activity
F	0017022	molecular_function	myosin binding
F	0019901	molecular_function	protein kinase binding
F	0021762	biological_process	substantia nigra development
F	0030027	cellular_component	lamellipodium
F	0030036	biological_process	actin cytoskeleton organization
F	0030054	cellular_component	cell junction
F	0030334	biological_process	regulation of cell migration
F	0030335	biological_process	positive regulation of cell migration
F	0030425	cellular_component	dendrite
F	0030496	cellular_component	midbody
F	0030667	cellular_component	secretory granule membrane
F	0031122	biological_process	cytoplasmic microtubule organization
F	0031982	cellular_component	vesicle
F	0032154	cellular_component	cleavage furrow
F	0032467	biological_process	positive regulation of cytokinesis
F	0032587	cellular_component	ruffle membrane
F	0032956	biological_process	regulation of actin cytoskeleton organization
F	0033688	biological_process	regulation of osteoblast proliferation
F	0034329	biological_process	cell junction assembly
F	0034446	biological_process	substrate adhesion-dependent cell spreading
F	0035385	biological_process	Roundabout signaling pathway
F	0036089	biological_process	cleavage furrow formation
F	0038027	biological_process	apolipoprotein A-I-mediated signaling pathway
F	0042476	biological_process	odontogenesis
F	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
F	0043149	biological_process	stress fiber assembly
F	0043197	cellular_component	dendritic spine
F	0043296	cellular_component	apical junction complex
F	0043297	biological_process	apical junction assembly
F	0043366	biological_process	beta selection
F	0043542	biological_process	endothelial cell migration
F	0043931	biological_process	ossification involved in bone maturation
F	0044319	biological_process	wound healing, spreading of cells
F	0045198	biological_process	establishment of epithelial cell apical/basal polarity
F	0045666	biological_process	positive regulation of neuron differentiation
F	0045792	biological_process	negative regulation of cell size
F	0046638	biological_process	positive regulation of alpha-beta T cell differentiation
F	0050919	biological_process	negative chemotaxis
F	0051301	biological_process	cell division
F	0051496	biological_process	positive regulation of stress fiber assembly
F	0051893	biological_process	regulation of focal adhesion assembly
F	0060071	biological_process	Wnt signaling pathway, planar cell polarity pathway
F	0060193	biological_process	positive regulation of lipase activity
F	0061430	biological_process	bone trabecula morphogenesis
F	0070062	cellular_component	extracellular exosome
F	0070507	biological_process	regulation of microtubule cytoskeleton organization
F	0071222	biological_process	cellular response to lipopolysaccharide
F	0071345	biological_process	cellular response to cytokine stimulus
F	0071526	biological_process	semaphorin-plexin signaling pathway
F	0071902	biological_process	positive regulation of protein serine/threonine kinase activity
F	0071944	cellular_component	cell periphery
F	0090051	biological_process	negative regulation of cell migration involved in sprouting angiogenesis
F	0090307	biological_process	mitotic spindle assembly
F	0090324	biological_process	negative regulation of oxidative phosphorylation
F	0097498	biological_process	endothelial tube lumen extension
F	0098794	cellular_component	postsynapse
F	0098978	cellular_component	glutamatergic synapse
F	0099159	biological_process	regulation of modification of postsynaptic structure
F	0101003	cellular_component	ficolin-1-rich granule membrane
F	1901224	biological_process	positive regulation of non-canonical NF-kappaB signal transduction
F	1902766	biological_process	skeletal muscle satellite cell migration
F	1903427	biological_process	negative regulation of reactive oxygen species biosynthetic process
F	1903673	biological_process	mitotic cleavage furrow formation
F	1904996	biological_process	positive regulation of leukocyte adhesion to vascular endothelial cell
F	1905274	biological_process	regulation of modification of postsynaptic actin cytoskeleton
F	1990869	biological_process	cellular response to chemokine
F	2000406	biological_process	positive regulation of T cell migration

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	420
Details	Transmembrane: {"description":"Helical","evidences":[{"source":"UniProtKB","id":"O35433","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	104
Details	Topological domain: {"description":"Extracellular","evidences":[{"source":"UniProtKB","id":"O35433","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	56
Details	Topological domain: {"description":"Cytoplasmic","evidences":[{"source":"UniProtKB","id":"O35433","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	76
Details	Intramembrane: {"description":"Pore-forming","evidences":[{"source":"UniProtKB","id":"O35433","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	116
Details	Repeat: {"description":"ANK 1"}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	116
Details	Repeat: {"description":"ANK 2"}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	116
Details	Repeat: {"description":"ANK 3"}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	12
Details	Motif: {"description":"Selectivity filter","evidences":[{"source":"UniProtKB","id":"O35433","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	28
Details	Binding site: {"evidences":[{"source":"PubMed","id":"22702953","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4DX2","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	24
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"A0A1D5PXA5","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"Q9R186","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	4
Details	Modified residue: {"description":"Phosphotyrosine","evidences":[{"source":"UniProtKB","id":"Q9EPK8","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI13
Number of Residues	34
Details	Region: {"description":"Switch II region; involved in RAP1GDS1 isoform 2 binding","evidences":[{"source":"PubMed","id":"30190425","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5ZHX","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI14
Number of Residues	16
Details	Motif: {"description":"Effector region","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI15
Number of Residues	20
Details	Binding site: {"evidences":[{"source":"PubMed","id":"10748207","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"12777804","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI16
Number of Residues	26
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"P62820","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI17
Number of Residues	2
Details	Modified residue: {"description":"(Microbial infection) O-AMP-tyrosine; by Haemophilus IbpA; alternate","evidences":[{"source":"PubMed","id":"19362538","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI18
Number of Residues	2
Details	Modified residue: {"description":"(Microbial infection) O-AMP-threonine; by Vibrio VopS","evidences":[{"source":"PubMed","id":"19039103","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI19
Number of Residues	2
Details	Modified residue: {"description":"(Microbial infection) ADP-ribosylasparagine; by botulinum toxin","evidences":[{"source":"PubMed","id":"1328215","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI20
Number of Residues	2
Details	Modified residue: {"description":"5-glutamyl serotonin","evidences":[{"source":"UniProtKB","id":"Q9QUI0","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI21
Number of Residues	2
Details	Glycosylation: {"description":"(Microbial infection) O-linked (GlcNAc) tyrosine; by Photorhabdus PAU_02230; alternate","evidences":[{"source":"PubMed","id":"24141704","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI22
Number of Residues	2
Details	Glycosylation: {"description":"(Microbial infection) O-linked (Glc) threonine; by C.difficile toxins TcdA and TcdB; alternate","evidences":[{"source":"PubMed","id":"24905543","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"7775453","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"7777059","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI23
Number of Residues	4
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"PubMed","id":"23871831","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---