9IML
Sertraline enhances the deubiquitinase activity of USP7 by binding to its switching loop region
Functional Information from PROSITE/UniProt
| site_id | PS00299 |
| Number of Residues | 26 |
| Details | UBIQUITIN_1 Ubiquitin domain signature. KakIqDkegIPpdqQrLIFaGkqleD |
| Chain | Residue | Details |
| B | LYS27-ASP52 |
| site_id | PS00972 |
| Number of Residues | 16 |
| Details | USP_1 Ubiquitin specific protease (USP) domain signature 1. GLknqGAtCYMNSlLQ |
| Chain | Residue | Details |
| A | GLY215-GLN230 |
| site_id | PS00973 |
| Number of Residues | 18 |
| Details | USP_2 Ubiquitin specific protease (USP) domain signature 2. YiLhAVlvHsGdnhg..GHY |
| Chain | Residue | Details |
| A | TYR448-TYR465 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | ACT_SITE: Nucleophile => ECO:0000269|PubMed:12507430, ECO:0000269|PubMed:25944111, ECO:0000305|PubMed:11923872, ECO:0000305|PubMed:15053880, ECO:0000305|PubMed:16964248, ECO:0000305|PubMed:18716620, ECO:0000305|PubMed:21745816, ECO:0000305|PubMed:22411829 |
| Chain | Residue | Details |
| A | CYS223 | |
| C | CYS223 | |
| E | CYS223 | |
| G | CYS223 | |
| F | ARG54 | |
| F | ARG72 | |
| H | ARG54 | |
| H | ARG72 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | ACT_SITE: Proton acceptor => ECO:0000305|PubMed:12507430 |
| Chain | Residue | Details |
| A | HIS464 | |
| C | HIS464 | |
| E | HIS464 | |
| G | HIS464 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 4 |
| Details | MOD_RES: Phosphoserine; by PINK1 => ECO:0000269|PubMed:24660806, ECO:0000269|PubMed:24751536, ECO:0000269|PubMed:24784582, ECO:0000269|PubMed:25527291 |
| Chain | Residue | Details |
| B | SER65 | |
| D | SER65 | |
| F | SER65 | |
| H | SER65 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 4 |
| Details | MOD_RES: (Microbial infection) ADP-ribosylthreonine => ECO:0000269|PubMed:32330457 |
| Chain | Residue | Details |
| B | THR66 | |
| D | THR66 | |
| F | THR66 | |
| H | THR66 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 4 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16443603 |
| Chain | Residue | Details |
| B | LYS6 | |
| D | LYS6 | |
| F | LYS6 | |
| H | LYS6 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 8 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16543144, ECO:0000269|PubMed:18719106 |
| Chain | Residue | Details |
| B | LYS63 | |
| D | LYS63 | |
| F | LYS63 | |
| H | LYS63 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 8 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16443603, ECO:0000269|PubMed:16543144 |
| Chain | Residue | Details |
| B | LYS11 | |
| B | LYS48 | |
| D | LYS11 | |
| D | LYS48 | |
| F | LYS11 | |
| F | LYS48 | |
| H | LYS11 | |
| H | LYS48 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 4 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000305|PubMed:15466860 |
| Chain | Residue | Details |
| B | LYS27 | |
| D | LYS27 | |
| F | LYS27 | |
| H | LYS27 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 4 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16543144, ECO:0000269|PubMed:25752573, ECO:0000269|PubMed:25752577, ECO:0000269|PubMed:34239127 |
| Chain | Residue | Details |
| B | LYS29 | |
| D | LYS29 | |
| F | LYS29 | |
| H | LYS29 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 4 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:25752577 |
| Chain | Residue | Details |
| B | LYS33 | |
| D | LYS33 | |
| F | LYS33 | |
| H | LYS33 |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 4 |
| Details | M-CSA 789 |
| Chain | Residue | Details |
| A | ASN218 | electrostatic stabiliser |
| A | CYS223 | nucleofuge, nucleophile, proton acceptor, proton donor |
| A | HIS464 | proton acceptor, proton donor |
| A | ASP481 | electrostatic stabiliser |
| site_id | MCSA2 |
| Number of Residues | 4 |
| Details | M-CSA 789 |
| Chain | Residue | Details |
| C | ASN218 | electrostatic stabiliser |
| C | CYS223 | nucleofuge, nucleophile, proton acceptor, proton donor |
| C | HIS464 | proton acceptor, proton donor |
| C | ASP481 | electrostatic stabiliser |
| site_id | MCSA3 |
| Number of Residues | 4 |
| Details | M-CSA 789 |
| Chain | Residue | Details |
| E | ASN218 | electrostatic stabiliser |
| E | CYS223 | nucleofuge, nucleophile, proton acceptor, proton donor |
| E | HIS464 | proton acceptor, proton donor |
| E | ASP481 | electrostatic stabiliser |
| site_id | MCSA4 |
| Number of Residues | 4 |
| Details | M-CSA 789 |
| Chain | Residue | Details |
| G | ASN218 | electrostatic stabiliser |
| G | CYS223 | nucleofuge, nucleophile, proton acceptor, proton donor |
| G | HIS464 | proton acceptor, proton donor |
| G | ASP481 | electrostatic stabiliser |
