9II3
Cryo-EM Structure of the 2:1 Complex of mGlu3 and beta-arrestin1
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000139 | cellular_component | Golgi membrane |
| A | 0000785 | cellular_component | chromatin |
| A | 0001664 | molecular_function | G protein-coupled receptor binding |
| A | 0001934 | biological_process | positive regulation of protein phosphorylation |
| A | 0002031 | biological_process | G protein-coupled receptor internalization |
| A | 0002092 | biological_process | positive regulation of receptor internalization |
| A | 0003713 | molecular_function | transcription coactivator activity |
| A | 0004402 | molecular_function | histone acetyltransferase activity |
| A | 0004857 | molecular_function | enzyme inhibitor activity |
| A | 0005096 | molecular_function | GTPase activator activity |
| A | 0005159 | molecular_function | insulin-like growth factor receptor binding |
| A | 0005515 | molecular_function | protein binding |
| A | 0005634 | cellular_component | nucleus |
| A | 0005654 | cellular_component | nucleoplasm |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005765 | cellular_component | lysosomal membrane |
| A | 0005829 | cellular_component | cytosol |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0005905 | cellular_component | clathrin-coated pit |
| A | 0006357 | biological_process | regulation of transcription by RNA polymerase II |
| A | 0006511 | biological_process | ubiquitin-dependent protein catabolic process |
| A | 0009968 | biological_process | negative regulation of signal transduction |
| A | 0010613 | biological_process | positive regulation of cardiac muscle hypertrophy |
| A | 0015031 | biological_process | protein transport |
| A | 0016020 | cellular_component | membrane |
| A | 0016567 | biological_process | protein ubiquitination |
| A | 0016604 | cellular_component | nuclear body |
| A | 0030659 | cellular_component | cytoplasmic vesicle membrane |
| A | 0030666 | cellular_component | endocytic vesicle membrane |
| A | 0031143 | cellular_component | pseudopodium |
| A | 0031397 | biological_process | negative regulation of protein ubiquitination |
| A | 0031410 | cellular_component | cytoplasmic vesicle |
| A | 0031625 | molecular_function | ubiquitin protein ligase binding |
| A | 0031701 | molecular_function | angiotensin receptor binding |
| A | 0032088 | biological_process | negative regulation of NF-kappaB transcription factor activity |
| A | 0032715 | biological_process | negative regulation of interleukin-6 production |
| A | 0032717 | biological_process | negative regulation of interleukin-8 production |
| A | 0035025 | biological_process | positive regulation of Rho protein signal transduction |
| A | 0042995 | cellular_component | cell projection |
| A | 0043149 | biological_process | stress fiber assembly |
| A | 0043161 | biological_process | proteasome-mediated ubiquitin-dependent protein catabolic process |
| A | 0045746 | biological_process | negative regulation of Notch signaling pathway |
| A | 0045944 | biological_process | positive regulation of transcription by RNA polymerase II |
| A | 0060090 | molecular_function | molecular adaptor activity |
| A | 0070374 | biological_process | positive regulation of ERK1 and ERK2 cascade |
| A | 1990763 | molecular_function | arrestin family protein binding |
Functional Information from PROSITE/UniProt
| site_id | PS00295 |
| Number of Residues | 19 |
| Details | ARRESTINS Arrestins signature. FRYGrEDlDVLGLtFrKDL |
| Chain | Residue | Details |
| A | PHE61-LEU79 |
| site_id | PS00979 |
| Number of Residues | 19 |
| Details | G_PROTEIN_RECEP_F3_1 G-protein coupled receptors family 3 signature 1. VaNLLrLFqIPQISyASTS |
| Chain | Residue | Details |
| R | VAL157-SER175 |
| site_id | PS00980 |
| Number of Residues | 23 |
| Details | G_PROTEIN_RECEP_F3_2 G-protein coupled receptors family 3 signature 2. CCWiCipCepyeYla...DefTCmdC |
| Chain | Residue | Details |
| R | CYS527-CYS549 |
| site_id | PS00981 |
| Number of Residues | 11 |
| Details | G_PROTEIN_RECEP_F3_3 G-protein coupled receptors family 3 signature 3. FNEAKfIGFTM |
| Chain | Residue | Details |
| R | PHE765-MET775 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000250 |
| Chain | Residue | Details |
| A | LYS250 | |
| A | MET255 | |
| A | LYS324 | |
| A | LYS326 | |
| B | LEU23-TRP576 | |
| B | LYS635-ARG645 | |
| B | GLU710-ASP734 | |
| B | SER793-THR802 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphotyrosine => ECO:0000250|UniProtKB:Q8BWG8 |
| Chain | Residue | Details |
| A | TYR47 | |
| B | ALA577-ILE599 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphoserine; by GRK5 => ECO:0000269|PubMed:19661922, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:23186163, ECO:0007744|PubMed:24275569 |
| Chain | Residue | Details |
| A | SER412 | |
| R | CYS665-GLN688 | |
| R | LYS757-LYS769 | |
| R | GLN829-LEU879 | |
| B | LYS600-GLU613 | |
| B | CYS665-GLN688 | |
| B | LYS757-LYS769 | |
| B | GLN829-LEU879 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 40 |
| Details | TRANSMEM: Helical; Name=2 => ECO:0000255 |
| Chain | Residue | Details |
| R | LEU614-ALA634 | |
| B | LEU614-ALA634 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 36 |
| Details | TRANSMEM: Helical; Name=3 => ECO:0000255 |
| Chain | Residue | Details |
| R | LEU646-ASN664 | |
| B | LEU646-ASN664 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 40 |
| Details | TRANSMEM: Helical; Name=4 => ECO:0000255 |
| Chain | Residue | Details |
| R | VAL689-LEU709 | |
| B | VAL689-LEU709 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 42 |
| Details | TRANSMEM: Helical; Name=5 => ECO:0000255 |
| Chain | Residue | Details |
| R | SER735-PHE756 | |
| B | SER735-PHE756 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 44 |
| Details | TRANSMEM: Helical; Name=6 => ECO:0000255 |
| Chain | Residue | Details |
| R | PHE770-THR792 | |
| B | PHE770-THR792 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 50 |
| Details | TRANSMEM: Helical; Name=7 => ECO:0000255 |
| Chain | Residue | Details |
| R | MET803-PHE828 | |
| B | MET803-PHE828 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 10 |
| Details | BINDING: BINDING => ECO:0000250 |
| Chain | Residue | Details |
| R | SER151 | |
| B | LYS389 | |
| R | ALA172 | |
| R | TYR222 | |
| R | ASP301 | |
| R | LYS389 | |
| B | SER151 | |
| B | ALA172 | |
| B | TYR222 | |
| B | ASP301 |
| site_id | SWS_FT_FI11 |
| Number of Residues | 8 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255 |
| Chain | Residue | Details |
| R | ASN209 | |
| R | ASN292 | |
| R | ASN414 | |
| R | ASN439 | |
| B | ASN209 | |
| B | ASN292 | |
| B | ASN414 | |
| B | ASN439 |
