9DW7

Dephosphorylated CFTR in 1:2 complex with PKA-C

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0005254	molecular_function	chloride channel activity
A	0005260	molecular_function	intracellularly ATP-gated chloride channel activity
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005765	cellular_component	lysosomal membrane
A	0005768	cellular_component	endosome
A	0005769	cellular_component	early endosome
A	0005783	cellular_component	endoplasmic reticulum
A	0005789	cellular_component	endoplasmic reticulum membrane
A	0005829	cellular_component	cytosol
A	0005886	cellular_component	plasma membrane
A	0006695	biological_process	cholesterol biosynthetic process
A	0006811	biological_process	monoatomic ion transport
A	0006821	biological_process	chloride transport
A	0006833	biological_process	water transport
A	0006904	biological_process	vesicle docking involved in exocytosis
A	0009986	cellular_component	cell surface
A	0010008	cellular_component	endosome membrane
A	0015106	molecular_function	bicarbonate transmembrane transporter activity
A	0015108	molecular_function	chloride transmembrane transporter activity
A	0015701	biological_process	bicarbonate transport
A	0016020	cellular_component	membrane
A	0016323	cellular_component	basolateral plasma membrane
A	0016324	cellular_component	apical plasma membrane
A	0016853	molecular_function	isomerase activity
A	0016887	molecular_function	ATP hydrolysis activity
A	0017081	molecular_function	chloride channel regulator activity
A	0019869	molecular_function	chloride channel inhibitor activity
A	0019899	molecular_function	enzyme binding
A	0030165	molecular_function	PDZ domain binding
A	0030301	biological_process	cholesterol transport
A	0030660	cellular_component	Golgi-associated vesicle membrane
A	0030669	cellular_component	clathrin-coated endocytic vesicle membrane
A	0031901	cellular_component	early endosome membrane
A	0032991	cellular_component	protein-containing complex
A	0034220	biological_process	monoatomic ion transmembrane transport
A	0034707	cellular_component	chloride channel complex
A	0034976	biological_process	response to endoplasmic reticulum stress
A	0035377	biological_process	transepithelial water transport
A	0035774	biological_process	positive regulation of insulin secretion involved in cellular response to glucose stimulus
A	0043225	molecular_function	ATPase-coupled inorganic anion transmembrane transporter activity
A	0045921	biological_process	positive regulation of exocytosis
A	0048240	biological_process	sperm capacitation
A	0050891	biological_process	multicellular organismal-level water homeostasis
A	0051087	molecular_function	protein-folding chaperone binding
A	0051454	biological_process	intracellular pH elevation
A	0051649	biological_process	establishment of localization in cell
A	0055037	cellular_component	recycling endosome
A	0055038	cellular_component	recycling endosome membrane
A	0055085	biological_process	transmembrane transport
A	0060081	biological_process	membrane hyperpolarization
A	0065008	biological_process	regulation of biological quality
A	0070175	biological_process	positive regulation of enamel mineralization
A	0071320	biological_process	cellular response to cAMP
A	0071889	molecular_function	14-3-3 protein binding
A	0097186	biological_process	amelogenesis
A	0098660	biological_process	inorganic ion transmembrane transport
A	0106138	molecular_function	Sec61 translocon complex binding
A	0140359	molecular_function	ABC-type transporter activity
A	1902476	biological_process	chloride transmembrane transport
A	1902943	biological_process	positive regulation of voltage-gated chloride channel activity
A	1904322	biological_process	cellular response to forskolin
G	0000166	molecular_function	nucleotide binding
G	0000287	molecular_function	magnesium ion binding
G	0001669	cellular_component	acrosomal vesicle
G	0001707	biological_process	mesoderm formation
G	0001843	biological_process	neural tube closure
G	0004672	molecular_function	protein kinase activity
G	0004674	molecular_function	protein serine/threonine kinase activity
G	0004691	molecular_function	cAMP-dependent protein kinase activity
G	0004712	molecular_function	protein serine/threonine/tyrosine kinase activity
G	0005515	molecular_function	protein binding
G	0005524	molecular_function	ATP binding
G	0005634	cellular_component	nucleus
G	0005737	cellular_component	cytoplasm
G	0005739	cellular_component	mitochondrion
G	0005813	cellular_component	centrosome
G	0005829	cellular_component	cytosol
G	0005886	cellular_component	plasma membrane
G	0005930	cellular_component	axoneme
G	0005952	cellular_component	cAMP-dependent protein kinase complex
G	0006397	biological_process	mRNA processing
G	0006468	biological_process	protein phosphorylation
G	0006611	biological_process	protein export from nucleus
G	0007189	biological_process	adenylate cyclase-activating G protein-coupled receptor signaling pathway
G	0007193	biological_process	adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway
G	0016020	cellular_component	membrane
G	0016301	molecular_function	kinase activity
G	0016607	cellular_component	nuclear speck
G	0016740	molecular_function	transferase activity
G	0018105	biological_process	peptidyl-serine phosphorylation
G	0019901	molecular_function	protein kinase binding
G	0019904	molecular_function	protein domain specific binding
G	0030007	biological_process	intracellular potassium ion homeostasis
G	0030145	molecular_function	manganese ion binding
G	0031594	cellular_component	neuromuscular junction
G	0031625	molecular_function	ubiquitin protein ligase binding
G	0032024	biological_process	positive regulation of insulin secretion
G	0034237	molecular_function	protein kinase A regulatory subunit binding
G	0034605	biological_process	cellular response to heat
G	0036126	cellular_component	sperm flagellum
G	0044853	cellular_component	plasma membrane raft
G	0045542	biological_process	positive regulation of cholesterol biosynthetic process
G	0045667	biological_process	regulation of osteoblast differentiation
G	0045722	biological_process	positive regulation of gluconeogenesis
G	0045879	biological_process	negative regulation of smoothened signaling pathway
G	0046827	biological_process	positive regulation of protein export from nucleus
G	0048240	biological_process	sperm capacitation
G	0048471	cellular_component	perinuclear region of cytoplasm
G	0050766	biological_process	positive regulation of phagocytosis
G	0050804	biological_process	modulation of chemical synaptic transmission
G	0051726	biological_process	regulation of cell cycle
G	0061136	biological_process	regulation of proteasomal protein catabolic process
G	0070417	biological_process	cellular response to cold
G	0070613	biological_process	regulation of protein processing
G	0071333	biological_process	cellular response to glucose stimulus
G	0071374	biological_process	cellular response to parathyroid hormone stimulus
G	0071377	biological_process	cellular response to glucagon stimulus
G	0097546	cellular_component	ciliary base
G	0098794	cellular_component	postsynapse
G	0098978	cellular_component	glutamatergic synapse
G	0099170	biological_process	postsynaptic modulation of chemical synaptic transmission
G	0106310	molecular_function	protein serine kinase activity
G	1904262	biological_process	negative regulation of TORC1 signaling
G	1904539	biological_process	negative regulation of glycolytic process through fructose-6-phosphate
G	1990044	biological_process	protein localization to lipid droplet
G	2000810	biological_process	regulation of bicellular tight junction assembly
K	0000166	molecular_function	nucleotide binding
K	0000287	molecular_function	magnesium ion binding
K	0001669	cellular_component	acrosomal vesicle
K	0001707	biological_process	mesoderm formation
K	0001843	biological_process	neural tube closure
K	0004672	molecular_function	protein kinase activity
K	0004674	molecular_function	protein serine/threonine kinase activity
K	0004691	molecular_function	cAMP-dependent protein kinase activity
K	0004712	molecular_function	protein serine/threonine/tyrosine kinase activity
K	0005515	molecular_function	protein binding
K	0005524	molecular_function	ATP binding
K	0005634	cellular_component	nucleus
K	0005737	cellular_component	cytoplasm
K	0005739	cellular_component	mitochondrion
K	0005813	cellular_component	centrosome
K	0005829	cellular_component	cytosol
K	0005886	cellular_component	plasma membrane
K	0005930	cellular_component	axoneme
K	0005952	cellular_component	cAMP-dependent protein kinase complex
K	0006397	biological_process	mRNA processing
K	0006468	biological_process	protein phosphorylation
K	0006611	biological_process	protein export from nucleus
K	0007189	biological_process	adenylate cyclase-activating G protein-coupled receptor signaling pathway
K	0007193	biological_process	adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway
K	0016020	cellular_component	membrane
K	0016301	molecular_function	kinase activity
K	0016607	cellular_component	nuclear speck
K	0016740	molecular_function	transferase activity
K	0018105	biological_process	peptidyl-serine phosphorylation
K	0019901	molecular_function	protein kinase binding
K	0019904	molecular_function	protein domain specific binding
K	0030007	biological_process	intracellular potassium ion homeostasis
K	0030145	molecular_function	manganese ion binding
K	0031594	cellular_component	neuromuscular junction
K	0031625	molecular_function	ubiquitin protein ligase binding
K	0032024	biological_process	positive regulation of insulin secretion
K	0034237	molecular_function	protein kinase A regulatory subunit binding
K	0034605	biological_process	cellular response to heat
K	0036126	cellular_component	sperm flagellum
K	0044853	cellular_component	plasma membrane raft
K	0045542	biological_process	positive regulation of cholesterol biosynthetic process
K	0045667	biological_process	regulation of osteoblast differentiation
K	0045722	biological_process	positive regulation of gluconeogenesis
K	0045879	biological_process	negative regulation of smoothened signaling pathway
K	0046827	biological_process	positive regulation of protein export from nucleus
K	0048240	biological_process	sperm capacitation
K	0048471	cellular_component	perinuclear region of cytoplasm
K	0050766	biological_process	positive regulation of phagocytosis
K	0050804	biological_process	modulation of chemical synaptic transmission
K	0051726	biological_process	regulation of cell cycle
K	0061136	biological_process	regulation of proteasomal protein catabolic process
K	0070417	biological_process	cellular response to cold
K	0070613	biological_process	regulation of protein processing
K	0071333	biological_process	cellular response to glucose stimulus
K	0071374	biological_process	cellular response to parathyroid hormone stimulus
K	0071377	biological_process	cellular response to glucagon stimulus
K	0097546	cellular_component	ciliary base
K	0098794	cellular_component	postsynapse
K	0098978	cellular_component	glutamatergic synapse
K	0099170	biological_process	postsynaptic modulation of chemical synaptic transmission
K	0106310	molecular_function	protein serine kinase activity
K	1904262	biological_process	negative regulation of TORC1 signaling
K	1904539	biological_process	negative regulation of glycolytic process through fructose-6-phosphate
K	1990044	biological_process	protein localization to lipid droplet
K	2000810	biological_process	regulation of bicellular tight junction assembly

Functional Information from PROSITE/UniProt

site_id	PS00107
Number of Residues	24
Details	PROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. LGTGSFGRVMlVkhmetgnh..........YAMK

Chain	Residue	Details
K	LEU50-LYS73

---

site_id	PS00108
Number of Residues	13
Details	PROTEIN_KINASE_ST Serine/Threonine protein kinases active-site signature. LiYrDLKpeNLLI

Chain	Residue	Details
K	LEU163-ILE175

---

site_id	PS00211
Number of Residues	15
Details	ABC_TRANSPORTER_1 ABC transporters family signature. LSGGQRARISLARAV

Chain	Residue	Details
A	LEU548-VAL562

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	76
Details	TOPO_DOM: Cytoplasmic => ECO:0000305

Chain	Residue	Details
A	MET1-PHE77
G	ASP167

---

site_id	SWS_FT_FI2
Number of Residues	20
Details	TRANSMEM: Helical; Name=1 => ECO:0000269|Ref.56

Chain	Residue	Details
A	PHE78-GLN98
K	LYS73
G	LEU50
G	LYS73

---

site_id	SWS_FT_FI3
Number of Residues	99
Details	TOPO_DOM: Extracellular => ECO:0000269|Ref.56

Chain	Residue	Details
A	PRO99-TYR122
A	GLU217-SER222
A	LEU320-THR339
A	VAL880-ILE918
A	GLN1012-PRO1013
A	ILE1117-GLY1130

---

site_id	SWS_FT_FI4
Number of Residues	23
Details	TRANSMEM: Helical; Name=2 => ECO:0000269|Ref.56

Chain	Residue	Details
A	LEU123-HIS146
G	ASN3

---

site_id	SWS_FT_FI5
Number of Residues	1039
Details	TOPO_DOM: Cytoplasmic => ECO:0000269|Ref.56

Chain	Residue	Details
A	HIS147-LEU195
A	MET244-LYS298
A	GLN359-SER858
A	THR940-THR990
A	GLN1035-THR1095
A	ASP1152-LEU1480

---

site_id	SWS_FT_FI6
Number of Residues	20
Details	TRANSMEM: Helical; Name=3 => ECO:0000269|Ref.56

Chain	Residue	Details
A	ALA196-TRP216
K	THR196
G	THR49
G	THR196

---

site_id	SWS_FT_FI7
Number of Residues	20
Details	TRANSMEM: Helical; Name=4 => ECO:0000269|Ref.56

Chain	Residue	Details
A	ALA223-MET243
G	SER140

---

site_id	SWS_FT_FI8
Number of Residues	20
Details	TRANSMEM: Helical; Name=5 => ECO:0000269|Ref.56

Chain	Residue	Details
A	ALA299-PHE319
G	THR198

---

site_id	SWS_FT_FI9
Number of Residues	18
Details	TRANSMEM: Helical; Name=6 => ECO:0000269|Ref.56

Chain	Residue	Details
A	ILE340-VAL358
G	TYR331

---

site_id	SWS_FT_FI10
Number of Residues	20
Details	TRANSMEM: Helical; Name=7 => ECO:0000269|Ref.56

Chain	Residue	Details
A	LEU859-VAL879
G	SER339

---

site_id	SWS_FT_FI11
Number of Residues	20
Details	TRANSMEM: Discontinuously helical; Name=8 => ECO:0000269|Ref.56

Chain	Residue	Details
A	TYR919-HIS939
G	GLY2

---

site_id	SWS_FT_FI12
Number of Residues	20
Details	TRANSMEM: Helical; Name=9 => ECO:0000269|Ref.56

Chain	Residue	Details
A	ILE991-LEU1011

---

site_id	SWS_FT_FI13
Number of Residues	20
Details	TRANSMEM: Helical; Name=10 => ECO:0000269|Ref.56

Chain	Residue	Details
A	TYR1014-LEU1034

---

site_id	SWS_FT_FI14
Number of Residues	20
Details	TRANSMEM: Helical; Name=11 => ECO:0000269|Ref.56

Chain	Residue	Details
A	LEU1096-PHE1116

---

site_id	SWS_FT_FI15
Number of Residues	20
Details	TRANSMEM: Helical; Name=12 => ECO:0000269|Ref.56

Chain	Residue	Details
A	ILE1131-ILE1151

---

site_id	SWS_FT_FI16
Number of Residues	1
Details	BINDING: BINDING => ECO:0000269|PubMed:15528182, ECO:0000269|PubMed:20150177, ECO:0007744|PDB:1XMI, ECO:0007744|PDB:1XMJ, ECO:0007744|PDB:2BBO, ECO:0007744|PDB:2BBS, ECO:0007744|PDB:2BBT, ECO:0007744|PDB:2PZE, ECO:0007744|PDB:2PZF, ECO:0007744|PDB:2PZG

Chain	Residue	Details
A	TRP401

---

site_id	SWS_FT_FI17
Number of Residues	1
Details	BINDING: BINDING => ECO:0000269|PubMed:20150177, ECO:0007744|PDB:2PZE, ECO:0007744|PDB:2PZF, ECO:0007744|PDB:2PZG

Chain	Residue	Details
A	SER434

---

site_id	SWS_FT_FI18
Number of Residues	1
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00434, ECO:0000269|PubMed:15528182, ECO:0007744|PDB:1XMI, ECO:0007744|PDB:1XMJ, ECO:0007744|PDB:2BBO, ECO:0007744|PDB:2BBS, ECO:0007744|PDB:2BBT

Chain	Residue	Details
A	GLY458

---

site_id	SWS_FT_FI19
Number of Residues	1
Details	BINDING: BINDING => ECO:0000269|PubMed:15528182, ECO:0007744|PDB:1XMI, ECO:0007744|PDB:2BBO, ECO:0007744|PDB:2BBS

Chain	Residue	Details
A	GLN493

---

site_id	SWS_FT_FI20
Number of Residues	1
Details	BINDING: BINDING => ECO:0007744|PDB:3GD7

Chain	Residue	Details
A	TYR1219

---

site_id	SWS_FT_FI21
Number of Residues	1
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00434, ECO:0007744|PDB:3GD7

Chain	Residue	Details
A	GLY1244

---

site_id	SWS_FT_FI22
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:20068231

Chain	Residue	Details
A	SER549

---

site_id	SWS_FT_FI23
Number of Residues	4
Details	MOD_RES: Phosphoserine; by PKA => ECO:0000269|PubMed:1377674, ECO:0000269|PubMed:22119790, ECO:0000269|PubMed:25330774, ECO:0000269|PubMed:9385646

Chain	Residue	Details
A	SER660
A	SER700
A	SER737
A	SER795

---

site_id	SWS_FT_FI24
Number of Residues	1
Details	MOD_RES: Phosphoserine; by PKA => ECO:0000269|PubMed:25330774

Chain	Residue	Details
A	SER670

---

site_id	SWS_FT_FI25
Number of Residues	1
Details	MOD_RES: Phosphoserine; by PKC => ECO:0000269|PubMed:1377674, ECO:0000269|PubMed:22119790

Chain	Residue	Details
A	SER686

---

site_id	SWS_FT_FI26
Number of Residues	1
Details	MOD_RES: Phosphoserine; by PKA => ECO:0000269|PubMed:22119790, ECO:0000269|PubMed:25330774, ECO:0000269|PubMed:9385646

Chain	Residue	Details
A	SER712

---

site_id	SWS_FT_FI27
Number of Residues	1
Details	MOD_RES: Phosphothreonine => ECO:0000269|PubMed:22119790

Chain	Residue	Details
A	THR717

---

site_id	SWS_FT_FI28
Number of Residues	1
Details	MOD_RES: Phosphoserine; by PKA => ECO:0000269|PubMed:25330774, ECO:0000269|PubMed:9385646

Chain	Residue	Details
A	SER753

---

site_id	SWS_FT_FI29
Number of Residues	2
Details	MOD_RES: Phosphoserine; by PKA => ECO:0000269|PubMed:1377674, ECO:0000269|PubMed:25330774, ECO:0000269|PubMed:9385646

Chain	Residue	Details
A	SER768
A	SER813

---

site_id	SWS_FT_FI30
Number of Residues	1
Details	MOD_RES: Phosphoserine; by PKC => ECO:0000269|PubMed:1377674

Chain	Residue	Details
A	SER790

---

site_id	SWS_FT_FI31
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0000269|PubMed:22119790

Chain	Residue	Details
A	SER1444
A	SER1456

---

site_id	SWS_FT_FI32
Number of Residues	2
Details	LIPID: S-palmitoyl cysteine => ECO:0000269|PubMed:22119790

Chain	Residue	Details
A	CYS524
A	CYS1395

---

site_id	SWS_FT_FI33
Number of Residues	2
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:7518437, ECO:0000305|PubMed:20008117

Chain	Residue	Details
A	ASN894
A	ASN900

---

site_id	SWS_FT_FI34
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:22119790

Chain	Residue	Details
A	LYS688

---

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	5
Details	M-CSA 757

Chain	Residue	Details
A	LEU167	activator, proton acceptor, proton donor
A	SER169	electrostatic stabiliser, polar interaction
A	LEU172	metal ligand
A	SER185	metal ligand
A	TRP202	electrostatic stabiliser, polar interaction

---

site_id	MCSA2
Number of Residues	5
Details	M-CSA 757

Chain

Residue

Details

---