9CAQ

Cryo-EM structure of a human MCM2-7 double hexamer formed from independently loaded MCM2-7 single hexamers

This is a non-PDB format compatible entry.

Functional Information from GO Data

Chain	GOid	namespace	contents
2	0000166	molecular_function	nucleotide binding
2	0000228	cellular_component	nuclear chromosome
2	0000727	biological_process	double-strand break repair via break-induced replication
2	0000781	cellular_component	chromosome, telomeric region
2	0000785	cellular_component	chromatin
2	0003677	molecular_function	DNA binding
2	0003678	molecular_function	DNA helicase activity
2	0003688	molecular_function	DNA replication origin binding
2	0003697	molecular_function	single-stranded DNA binding
2	0004386	molecular_function	helicase activity
2	0005515	molecular_function	protein binding
2	0005524	molecular_function	ATP binding
2	0005634	cellular_component	nucleus
2	0005654	cellular_component	nucleoplasm
2	0005664	cellular_component	nuclear origin of replication recognition complex
2	0005694	cellular_component	chromosome
2	0005730	cellular_component	nucleolus
2	0005737	cellular_component	cytoplasm
2	0005829	cellular_component	cytosol
2	0005929	cellular_component	cilium
2	0006260	biological_process	DNA replication
2	0006270	biological_process	DNA replication initiation
2	0006334	biological_process	nucleosome assembly
2	0006915	biological_process	apoptotic process
2	0008270	molecular_function	zinc ion binding
2	0016787	molecular_function	hydrolase activity
2	0016887	molecular_function	ATP hydrolysis activity
2	0017116	molecular_function	single-stranded DNA helicase activity
2	0019899	molecular_function	enzyme binding
2	0030174	biological_process	regulation of DNA-templated DNA replication initiation
2	0042393	molecular_function	histone binding
2	0042555	cellular_component	MCM complex
2	0043138	molecular_function	3'-5' DNA helicase activity
2	0046872	molecular_function	metal ion binding
2	0071162	cellular_component	CMG complex
2	0071353	biological_process	cellular response to interleukin-4
2	0090102	biological_process	cochlea development
2	1902975	biological_process	mitotic DNA replication initiation
3	0000166	molecular_function	nucleotide binding
3	0000727	biological_process	double-strand break repair via break-induced replication
3	0000781	cellular_component	chromosome, telomeric region
3	0003677	molecular_function	DNA binding
3	0003678	molecular_function	DNA helicase activity
3	0003697	molecular_function	single-stranded DNA binding
3	0004386	molecular_function	helicase activity
3	0005515	molecular_function	protein binding
3	0005524	molecular_function	ATP binding
3	0005634	cellular_component	nucleus
3	0005654	cellular_component	nucleoplasm
3	0005658	cellular_component	alpha DNA polymerase:primase complex
3	0005694	cellular_component	chromosome
3	0005730	cellular_component	nucleolus
3	0005737	cellular_component	cytoplasm
3	0006260	biological_process	DNA replication
3	0006270	biological_process	DNA replication initiation
3	0006271	biological_process	DNA strand elongation involved in DNA replication
3	0016020	cellular_component	membrane
3	0016787	molecular_function	hydrolase activity
3	0016887	molecular_function	ATP hydrolysis activity
3	0017116	molecular_function	single-stranded DNA helicase activity
3	0030174	biological_process	regulation of DNA-templated DNA replication initiation
3	0042555	cellular_component	MCM complex
3	0048471	cellular_component	perinuclear region of cytoplasm
3	0071162	cellular_component	CMG complex
3	1902975	biological_process	mitotic DNA replication initiation
4	0000166	molecular_function	nucleotide binding
4	0000727	biological_process	double-strand break repair via break-induced replication
4	0000781	cellular_component	chromosome, telomeric region
4	0003677	molecular_function	DNA binding
4	0003678	molecular_function	DNA helicase activity
4	0003697	molecular_function	single-stranded DNA binding
4	0004386	molecular_function	helicase activity
4	0005515	molecular_function	protein binding
4	0005524	molecular_function	ATP binding
4	0005634	cellular_component	nucleus
4	0005654	cellular_component	nucleoplasm
4	0005694	cellular_component	chromosome
4	0006260	biological_process	DNA replication
4	0006270	biological_process	DNA replication initiation
4	0006271	biological_process	DNA strand elongation involved in DNA replication
4	0016020	cellular_component	membrane
4	0016787	molecular_function	hydrolase activity
4	0016887	molecular_function	ATP hydrolysis activity
4	0017116	molecular_function	single-stranded DNA helicase activity
4	0030174	biological_process	regulation of DNA-templated DNA replication initiation
4	0042555	cellular_component	MCM complex
4	0071162	cellular_component	CMG complex
4	1902975	biological_process	mitotic DNA replication initiation
5	0000166	molecular_function	nucleotide binding
5	0000727	biological_process	double-strand break repair via break-induced replication
5	0000781	cellular_component	chromosome, telomeric region
5	0003677	molecular_function	DNA binding
5	0003678	molecular_function	DNA helicase activity
5	0003688	molecular_function	DNA replication origin binding
5	0003697	molecular_function	single-stranded DNA binding
5	0004386	molecular_function	helicase activity
5	0005515	molecular_function	protein binding
5	0005524	molecular_function	ATP binding
5	0005634	cellular_component	nucleus
5	0005654	cellular_component	nucleoplasm
5	0005694	cellular_component	chromosome
5	0006260	biological_process	DNA replication
5	0006270	biological_process	DNA replication initiation
5	0016020	cellular_component	membrane
5	0016787	molecular_function	hydrolase activity
5	0016887	molecular_function	ATP hydrolysis activity
5	0017116	molecular_function	single-stranded DNA helicase activity
5	0030174	biological_process	regulation of DNA-templated DNA replication initiation
5	0042555	cellular_component	MCM complex
5	0043138	molecular_function	3'-5' DNA helicase activity
5	0071162	cellular_component	CMG complex
6	0000166	molecular_function	nucleotide binding
6	0000727	biological_process	double-strand break repair via break-induced replication
6	0000781	cellular_component	chromosome, telomeric region
6	0003677	molecular_function	DNA binding
6	0003678	molecular_function	DNA helicase activity
6	0003697	molecular_function	single-stranded DNA binding
6	0004386	molecular_function	helicase activity
6	0005515	molecular_function	protein binding
6	0005524	molecular_function	ATP binding
6	0005634	cellular_component	nucleus
6	0005654	cellular_component	nucleoplasm
6	0005694	cellular_component	chromosome
6	0005829	cellular_component	cytosol
6	0006260	biological_process	DNA replication
6	0006270	biological_process	DNA replication initiation
6	0016787	molecular_function	hydrolase activity
6	0016887	molecular_function	ATP hydrolysis activity
6	0030174	biological_process	regulation of DNA-templated DNA replication initiation
6	0042555	cellular_component	MCM complex
6	0042802	molecular_function	identical protein binding
6	0071162	cellular_component	CMG complex
6	1902969	biological_process	mitotic DNA replication
6	1990518	molecular_function	single-stranded 3'-5' DNA helicase activity
7	0000166	molecular_function	nucleotide binding
7	0000727	biological_process	double-strand break repair via break-induced replication
7	0000781	cellular_component	chromosome, telomeric region
7	0000785	cellular_component	chromatin
7	0003677	molecular_function	DNA binding
7	0003678	molecular_function	DNA helicase activity
7	0003697	molecular_function	single-stranded DNA binding
7	0004386	molecular_function	helicase activity
7	0005515	molecular_function	protein binding
7	0005524	molecular_function	ATP binding
7	0005634	cellular_component	nucleus
7	0005654	cellular_component	nucleoplasm
7	0005694	cellular_component	chromosome
7	0005730	cellular_component	nucleolus
7	0006260	biological_process	DNA replication
7	0006270	biological_process	DNA replication initiation
7	0006271	biological_process	DNA strand elongation involved in DNA replication
7	0006974	biological_process	DNA damage response
7	0008283	biological_process	cell population proliferation
7	0009410	biological_process	response to xenobiotic stimulus
7	0016020	cellular_component	membrane
7	0016787	molecular_function	hydrolase activity
7	0016887	molecular_function	ATP hydrolysis activity
7	0017116	molecular_function	single-stranded DNA helicase activity
7	0030174	biological_process	regulation of DNA-templated DNA replication initiation
7	0042325	biological_process	regulation of phosphorylation
7	0042555	cellular_component	MCM complex
7	0071162	cellular_component	CMG complex
7	0071364	biological_process	cellular response to epidermal growth factor stimulus
7	0071466	biological_process	cellular response to xenobiotic stimulus
A	0000166	molecular_function	nucleotide binding
A	0000228	cellular_component	nuclear chromosome
A	0000727	biological_process	double-strand break repair via break-induced replication
A	0000781	cellular_component	chromosome, telomeric region
A	0000785	cellular_component	chromatin
A	0003677	molecular_function	DNA binding
A	0003678	molecular_function	DNA helicase activity
A	0003688	molecular_function	DNA replication origin binding
A	0003697	molecular_function	single-stranded DNA binding
A	0004386	molecular_function	helicase activity
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005664	cellular_component	nuclear origin of replication recognition complex
A	0005694	cellular_component	chromosome
A	0005730	cellular_component	nucleolus
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0005929	cellular_component	cilium
A	0006260	biological_process	DNA replication
A	0006270	biological_process	DNA replication initiation
A	0006334	biological_process	nucleosome assembly
A	0006915	biological_process	apoptotic process
A	0008270	molecular_function	zinc ion binding
A	0016787	molecular_function	hydrolase activity
A	0016887	molecular_function	ATP hydrolysis activity
A	0017116	molecular_function	single-stranded DNA helicase activity
A	0019899	molecular_function	enzyme binding
A	0030174	biological_process	regulation of DNA-templated DNA replication initiation
A	0042393	molecular_function	histone binding
A	0042555	cellular_component	MCM complex
A	0043138	molecular_function	3'-5' DNA helicase activity
A	0046872	molecular_function	metal ion binding
A	0071162	cellular_component	CMG complex
A	0071353	biological_process	cellular response to interleukin-4
A	0090102	biological_process	cochlea development
A	1902975	biological_process	mitotic DNA replication initiation
B	0000166	molecular_function	nucleotide binding
B	0000727	biological_process	double-strand break repair via break-induced replication
B	0000781	cellular_component	chromosome, telomeric region
B	0003677	molecular_function	DNA binding
B	0003678	molecular_function	DNA helicase activity
B	0003697	molecular_function	single-stranded DNA binding
B	0004386	molecular_function	helicase activity
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005658	cellular_component	alpha DNA polymerase:primase complex
B	0005694	cellular_component	chromosome
B	0005730	cellular_component	nucleolus
B	0005737	cellular_component	cytoplasm
B	0006260	biological_process	DNA replication
B	0006270	biological_process	DNA replication initiation
B	0006271	biological_process	DNA strand elongation involved in DNA replication
B	0016020	cellular_component	membrane
B	0016787	molecular_function	hydrolase activity
B	0016887	molecular_function	ATP hydrolysis activity
B	0017116	molecular_function	single-stranded DNA helicase activity
B	0030174	biological_process	regulation of DNA-templated DNA replication initiation
B	0042555	cellular_component	MCM complex
B	0048471	cellular_component	perinuclear region of cytoplasm
B	0071162	cellular_component	CMG complex
B	1902975	biological_process	mitotic DNA replication initiation
C	0000166	molecular_function	nucleotide binding
C	0000727	biological_process	double-strand break repair via break-induced replication
C	0000781	cellular_component	chromosome, telomeric region
C	0003677	molecular_function	DNA binding
C	0003678	molecular_function	DNA helicase activity
C	0003697	molecular_function	single-stranded DNA binding
C	0004386	molecular_function	helicase activity
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005694	cellular_component	chromosome
C	0006260	biological_process	DNA replication
C	0006270	biological_process	DNA replication initiation
C	0006271	biological_process	DNA strand elongation involved in DNA replication
C	0016020	cellular_component	membrane
C	0016787	molecular_function	hydrolase activity
C	0016887	molecular_function	ATP hydrolysis activity
C	0017116	molecular_function	single-stranded DNA helicase activity
C	0030174	biological_process	regulation of DNA-templated DNA replication initiation
C	0042555	cellular_component	MCM complex
C	0071162	cellular_component	CMG complex
C	1902975	biological_process	mitotic DNA replication initiation
D	0000166	molecular_function	nucleotide binding
D	0000727	biological_process	double-strand break repair via break-induced replication
D	0000781	cellular_component	chromosome, telomeric region
D	0003677	molecular_function	DNA binding
D	0003678	molecular_function	DNA helicase activity
D	0003688	molecular_function	DNA replication origin binding
D	0003697	molecular_function	single-stranded DNA binding
D	0004386	molecular_function	helicase activity
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005694	cellular_component	chromosome
D	0006260	biological_process	DNA replication
D	0006270	biological_process	DNA replication initiation
D	0016020	cellular_component	membrane
D	0016787	molecular_function	hydrolase activity
D	0016887	molecular_function	ATP hydrolysis activity
D	0017116	molecular_function	single-stranded DNA helicase activity
D	0030174	biological_process	regulation of DNA-templated DNA replication initiation
D	0042555	cellular_component	MCM complex
D	0043138	molecular_function	3'-5' DNA helicase activity
D	0071162	cellular_component	CMG complex
E	0000166	molecular_function	nucleotide binding
E	0000727	biological_process	double-strand break repair via break-induced replication
E	0000781	cellular_component	chromosome, telomeric region
E	0003677	molecular_function	DNA binding
E	0003678	molecular_function	DNA helicase activity
E	0003697	molecular_function	single-stranded DNA binding
E	0004386	molecular_function	helicase activity
E	0005515	molecular_function	protein binding
E	0005524	molecular_function	ATP binding
E	0005634	cellular_component	nucleus
E	0005654	cellular_component	nucleoplasm
E	0005694	cellular_component	chromosome
E	0005829	cellular_component	cytosol
E	0006260	biological_process	DNA replication
E	0006270	biological_process	DNA replication initiation
E	0016787	molecular_function	hydrolase activity
E	0016887	molecular_function	ATP hydrolysis activity
E	0030174	biological_process	regulation of DNA-templated DNA replication initiation
E	0042555	cellular_component	MCM complex
E	0042802	molecular_function	identical protein binding
E	0071162	cellular_component	CMG complex
E	1902969	biological_process	mitotic DNA replication
E	1990518	molecular_function	single-stranded 3'-5' DNA helicase activity
F	0000166	molecular_function	nucleotide binding
F	0000727	biological_process	double-strand break repair via break-induced replication
F	0000781	cellular_component	chromosome, telomeric region
F	0000785	cellular_component	chromatin
F	0003677	molecular_function	DNA binding
F	0003678	molecular_function	DNA helicase activity
F	0003697	molecular_function	single-stranded DNA binding
F	0004386	molecular_function	helicase activity
F	0005515	molecular_function	protein binding
F	0005524	molecular_function	ATP binding
F	0005634	cellular_component	nucleus
F	0005654	cellular_component	nucleoplasm
F	0005694	cellular_component	chromosome
F	0005730	cellular_component	nucleolus
F	0006260	biological_process	DNA replication
F	0006270	biological_process	DNA replication initiation
F	0006271	biological_process	DNA strand elongation involved in DNA replication
F	0006974	biological_process	DNA damage response
F	0008283	biological_process	cell population proliferation
F	0009410	biological_process	response to xenobiotic stimulus
F	0016020	cellular_component	membrane
F	0016787	molecular_function	hydrolase activity
F	0016887	molecular_function	ATP hydrolysis activity
F	0017116	molecular_function	single-stranded DNA helicase activity
F	0030174	biological_process	regulation of DNA-templated DNA replication initiation
F	0042325	biological_process	regulation of phosphorylation
F	0042555	cellular_component	MCM complex
F	0071162	cellular_component	CMG complex
F	0071364	biological_process	cellular response to epidermal growth factor stimulus
F	0071466	biological_process	cellular response to xenobiotic stimulus

Functional Information from PROSITE/UniProt

site_id	PS00847
Number of Residues	9
Details	MCM_1 MCM family signature. GICCIDEFD

Chain	Residue	Details
4	GLY569-ASP577
2	GLY582-ASP590
5	GLY440-ASP448
3	GLY404-ASP412
6	GLY455-ASP463
7	GLY440-ASP448

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1863
Details	Domain: {"description":"MCM"}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	52
Details	Zinc finger: {"description":"C4-type","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	30
Details	Motif: {"description":"Arginine finger"}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PubMed","id":"32453425","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"6XTX","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	10
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"19608861","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"21406692","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	12
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	10
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)","evidences":[{"source":"PubMed","id":"28112733","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	62
Details	Binding site: {"evidences":[{"source":"PubMed","id":"32453425","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"6XTX","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"18669648","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"21406692","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	3
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"UniProtKB","id":"P25206","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	4
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI13
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"17081983","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"18691976","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"20068231","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"21406692","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI14
Number of Residues	2
Details	Modified residue: {"description":"N-acetylserine","evidences":[{"source":"PubMed","id":"19413330","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"22223895","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"22814378","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI15
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"16964243","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"18669648","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"19690332","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"20068231","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI16
Number of Residues	2
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"UniProtKB","id":"P97311","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI17
Number of Residues	43
Details	Region: {"description":"Interaction with RAD17","evidences":[{"source":"PubMed","id":"15538388","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI18
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"18669648","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"18691976","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"20068231","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI19
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"20068231","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---