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9AXA

CryoEM structure of activated CRAF/MEK/14-3-3 complex with NST-628

This is a non-PDB format compatible entry.
Functional Information from GO Data
ChainGOidnamespacecontents
A0004364molecular_functionglutathione transferase activity
A0004672molecular_functionprotein kinase activity
A0005524molecular_functionATP binding
A0006468biological_processprotein phosphorylation
A0006749biological_processglutathione metabolic process
A0016740molecular_functiontransferase activity
B0000165biological_processMAPK cascade
B0000166molecular_functionnucleotide binding
B0000187biological_processobsolete activation of MAPK activity
B0003056biological_processregulation of vascular associated smooth muscle contraction
B0004672molecular_functionprotein kinase activity
B0004674molecular_functionprotein serine/threonine kinase activity
B0004708molecular_functionMAP kinase kinase activity
B0004712molecular_functionprotein serine/threonine/tyrosine kinase activity
B0004713molecular_functionprotein tyrosine kinase activity
B0005078molecular_functionMAP-kinase scaffold activity
B0005515molecular_functionprotein binding
B0005524molecular_functionATP binding
B0005634cellular_componentnucleus
B0005737cellular_componentcytoplasm
B0005739cellular_componentmitochondrion
B0005769cellular_componentearly endosome
B0005770cellular_componentlate endosome
B0005783cellular_componentendoplasmic reticulum
B0005794cellular_componentGolgi apparatus
B0005813cellular_componentcentrosome
B0005829cellular_componentcytosol
B0005856cellular_componentcytoskeleton
B0005874cellular_componentmicrotubule
B0005886cellular_componentplasma membrane
B0005925cellular_componentfocal adhesion
B0005938cellular_componentcell cortex
B0006468biological_processprotein phosphorylation
B0006935biological_processchemotaxis
B0006979biological_processresponse to oxidative stress
B0007165biological_processsignal transduction
B0007507biological_processheart development
B0008285biological_processnegative regulation of cell population proliferation
B0010508biological_processpositive regulation of autophagy
B0010628biological_processpositive regulation of gene expression
B0010629biological_processnegative regulation of gene expression
B0014044biological_processSchwann cell development
B0014069cellular_componentpostsynaptic density
B0016020cellular_componentmembrane
B0016301molecular_functionkinase activity
B0016740molecular_functiontransferase activity
B0019901molecular_functionprotein kinase binding
B0021697biological_processcerebellar cortex formation
B0021953biological_processcentral nervous system neuron differentiation
B0030182biological_processneuron differentiation
B0030216biological_processkeratinocyte differentiation
B0030295molecular_functionprotein kinase activator activity
B0030335biological_processpositive regulation of cell migration
B0030424cellular_componentaxon
B0030425cellular_componentdendrite
B0030878biological_processthyroid gland development
B0031267molecular_functionsmall GTPase binding
B0031435molecular_functionmitogen-activated protein kinase kinase kinase binding
B0032402biological_processmelanosome transport
B0032839cellular_componentdendrite cytoplasm
B0032872biological_processregulation of stress-activated MAPK cascade
B0032968biological_processpositive regulation of transcription elongation by RNA polymerase II
B0034111biological_processnegative regulation of homotypic cell-cell adhesion
B0035987biological_processendodermal cell differentiation
B0036064cellular_componentciliary basal body
B0038133biological_processERBB2-ERBB3 signaling pathway
B0042552biological_processmyelination
B0043204cellular_componentperikaryon
B0043410biological_processpositive regulation of MAPK cascade
B0043539molecular_functionprotein serine/threonine kinase activator activity
B0044342biological_processtype B pancreatic cell proliferation
B0044877molecular_functionprotein-containing complex binding
B0045893biological_processpositive regulation of DNA-templated transcription
B0045933biological_processpositive regulation of muscle contraction
B0046579biological_processpositive regulation of Ras protein signal transduction
B0047496biological_processvesicle transport along microtubule
B0048009biological_processinsulin-like growth factor receptor signaling pathway
B0048313biological_processGolgi inheritance
B0048471cellular_componentperinuclear region of cytoplasm
B0048538biological_processthymus development
B0048678biological_processresponse to axon injury
B0048679biological_processregulation of axon regeneration
B0048812biological_processneuron projection morphogenesis
B0048870biological_processcell motility
B0050772biological_processpositive regulation of axonogenesis
B0051384biological_processresponse to glucocorticoid
B0060020biological_processBergmann glial cell differentiation
B0060324biological_processface development
B0060425biological_processlung morphogenesis
B0060440biological_processtrachea formation
B0060502biological_processepithelial cell proliferation involved in lung morphogenesis
B0060674biological_processplacenta blood vessel development
B0060711biological_processlabyrinthine layer development
B0070328biological_processtriglyceride homeostasis
B0070371biological_processERK1 and ERK2 cascade
B0070372biological_processregulation of ERK1 and ERK2 cascade
B0070374biological_processpositive regulation of ERK1 and ERK2 cascade
B0071902biological_processpositive regulation of protein serine/threonine kinase activity
B0090170biological_processregulation of Golgi inheritance
B0090398biological_processcellular senescence
B0097110molecular_functionscaffold protein binding
B0098696biological_processregulation of neurotransmitter receptor localization to postsynaptic specialization membrane
B0098978cellular_componentglutamatergic synapse
B0106310molecular_functionprotein serine kinase activity
B1903226biological_processpositive regulation of endodermal cell differentiation
B1903298biological_processnegative regulation of hypoxia-induced intrinsic apoptotic signaling pathway
B2000641biological_processregulation of early endosome to late endosome transport
B2001171biological_processpositive regulation of ATP biosynthetic process
C0004364molecular_functionglutathione transferase activity
C0004672molecular_functionprotein kinase activity
C0005524molecular_functionATP binding
C0006468biological_processprotein phosphorylation
C0006749biological_processglutathione metabolic process
C0016740molecular_functiontransferase activity
D0000165biological_processMAPK cascade
D0000166molecular_functionnucleotide binding
D0000187biological_processobsolete activation of MAPK activity
D0003056biological_processregulation of vascular associated smooth muscle contraction
D0004672molecular_functionprotein kinase activity
D0004674molecular_functionprotein serine/threonine kinase activity
D0004708molecular_functionMAP kinase kinase activity
D0004712molecular_functionprotein serine/threonine/tyrosine kinase activity
D0004713molecular_functionprotein tyrosine kinase activity
D0005078molecular_functionMAP-kinase scaffold activity
D0005515molecular_functionprotein binding
D0005524molecular_functionATP binding
D0005634cellular_componentnucleus
D0005737cellular_componentcytoplasm
D0005739cellular_componentmitochondrion
D0005769cellular_componentearly endosome
D0005770cellular_componentlate endosome
D0005783cellular_componentendoplasmic reticulum
D0005794cellular_componentGolgi apparatus
D0005813cellular_componentcentrosome
D0005829cellular_componentcytosol
D0005856cellular_componentcytoskeleton
D0005874cellular_componentmicrotubule
D0005886cellular_componentplasma membrane
D0005925cellular_componentfocal adhesion
D0005938cellular_componentcell cortex
D0006468biological_processprotein phosphorylation
D0006935biological_processchemotaxis
D0006979biological_processresponse to oxidative stress
D0007165biological_processsignal transduction
D0007507biological_processheart development
D0008285biological_processnegative regulation of cell population proliferation
D0010508biological_processpositive regulation of autophagy
D0010628biological_processpositive regulation of gene expression
D0010629biological_processnegative regulation of gene expression
D0014044biological_processSchwann cell development
D0014069cellular_componentpostsynaptic density
D0016020cellular_componentmembrane
D0016301molecular_functionkinase activity
D0016740molecular_functiontransferase activity
D0019901molecular_functionprotein kinase binding
D0021697biological_processcerebellar cortex formation
D0021953biological_processcentral nervous system neuron differentiation
D0030182biological_processneuron differentiation
D0030216biological_processkeratinocyte differentiation
D0030295molecular_functionprotein kinase activator activity
D0030335biological_processpositive regulation of cell migration
D0030424cellular_componentaxon
D0030425cellular_componentdendrite
D0030878biological_processthyroid gland development
D0031267molecular_functionsmall GTPase binding
D0031435molecular_functionmitogen-activated protein kinase kinase kinase binding
D0032402biological_processmelanosome transport
D0032839cellular_componentdendrite cytoplasm
D0032872biological_processregulation of stress-activated MAPK cascade
D0032968biological_processpositive regulation of transcription elongation by RNA polymerase II
D0034111biological_processnegative regulation of homotypic cell-cell adhesion
D0035987biological_processendodermal cell differentiation
D0036064cellular_componentciliary basal body
D0038133biological_processERBB2-ERBB3 signaling pathway
D0042552biological_processmyelination
D0043204cellular_componentperikaryon
D0043410biological_processpositive regulation of MAPK cascade
D0043539molecular_functionprotein serine/threonine kinase activator activity
D0044342biological_processtype B pancreatic cell proliferation
D0044877molecular_functionprotein-containing complex binding
D0045893biological_processpositive regulation of DNA-templated transcription
D0045933biological_processpositive regulation of muscle contraction
D0046579biological_processpositive regulation of Ras protein signal transduction
D0047496biological_processvesicle transport along microtubule
D0048009biological_processinsulin-like growth factor receptor signaling pathway
D0048313biological_processGolgi inheritance
D0048471cellular_componentperinuclear region of cytoplasm
D0048538biological_processthymus development
D0048678biological_processresponse to axon injury
D0048679biological_processregulation of axon regeneration
D0048812biological_processneuron projection morphogenesis
D0048870biological_processcell motility
D0050772biological_processpositive regulation of axonogenesis
D0051384biological_processresponse to glucocorticoid
D0060020biological_processBergmann glial cell differentiation
D0060324biological_processface development
D0060425biological_processlung morphogenesis
D0060440biological_processtrachea formation
D0060502biological_processepithelial cell proliferation involved in lung morphogenesis
D0060674biological_processplacenta blood vessel development
D0060711biological_processlabyrinthine layer development
D0070328biological_processtriglyceride homeostasis
D0070371biological_processERK1 and ERK2 cascade
D0070372biological_processregulation of ERK1 and ERK2 cascade
D0070374biological_processpositive regulation of ERK1 and ERK2 cascade
D0071902biological_processpositive regulation of protein serine/threonine kinase activity
D0090170biological_processregulation of Golgi inheritance
D0090398biological_processcellular senescence
D0097110molecular_functionscaffold protein binding
D0098696biological_processregulation of neurotransmitter receptor localization to postsynaptic specialization membrane
D0098978cellular_componentglutamatergic synapse
D0106310molecular_functionprotein serine kinase activity
D1903226biological_processpositive regulation of endodermal cell differentiation
D1903298biological_processnegative regulation of hypoxia-induced intrinsic apoptotic signaling pathway
D2000641biological_processregulation of early endosome to late endosome transport
D2001171biological_processpositive regulation of ATP biosynthetic process
E0000122biological_processnegative regulation of transcription by RNA polymerase II
E0001836biological_processrelease of cytochrome c from mitochondria
E0003334biological_processkeratinocyte development
E0005515molecular_functionprotein binding
E0005576cellular_componentextracellular region
E0005615cellular_componentextracellular space
E0005634cellular_componentnucleus
E0005737cellular_componentcytoplasm
E0005829cellular_componentcytosol
E0006469biological_processnegative regulation of protein kinase activity
E0006611biological_processprotein export from nucleus
E0007165biological_processsignal transduction
E0008104biological_processprotein localization
E0008426molecular_functionprotein kinase C inhibitor activity
E0008630biological_processintrinsic apoptotic signaling pathway in response to DNA damage
E0010482biological_processregulation of epidermal cell division
E0010839biological_processnegative regulation of keratinocyte proliferation
E0019901molecular_functionprotein kinase binding
E0022407biological_processregulation of cell-cell adhesion
E0030216biological_processkeratinocyte differentiation
E0030307biological_processpositive regulation of cell growth
E0031424biological_processkeratinization
E0032880biological_processregulation of protein localization
E0042742biological_processdefense response to bacterium
E0042802molecular_functionidentical protein binding
E0043588biological_processskin development
E0043616biological_processkeratinocyte proliferation
E0045296molecular_functioncadherin binding
E0045606biological_processpositive regulation of epidermal cell differentiation
E0045785biological_processpositive regulation of cell adhesion
E0045824biological_processnegative regulation of innate immune response
E0046827biological_processpositive regulation of protein export from nucleus
E0050815molecular_functionphosphoserine residue binding
E0051219molecular_functionphosphoprotein binding
E0051715biological_processcytolysis in another organism
E0051726biological_processregulation of cell cycle
E0061436biological_processestablishment of skin barrier
E0070062cellular_componentextracellular exosome
E0072089biological_processstem cell proliferation
E0140311molecular_functionprotein sequestering activity
E0141156biological_processcAMP/PKA signal transduction
E0160075biological_processnon-canonical inflammasome complex assembly
E1903077biological_processnegative regulation of protein localization to plasma membrane
E1903829biological_processpositive regulation of protein localization
E2000647biological_processnegative regulation of stem cell proliferation
F0000122biological_processnegative regulation of transcription by RNA polymerase II
F0001836biological_processrelease of cytochrome c from mitochondria
F0003334biological_processkeratinocyte development
F0005515molecular_functionprotein binding
F0005576cellular_componentextracellular region
F0005615cellular_componentextracellular space
F0005634cellular_componentnucleus
F0005737cellular_componentcytoplasm
F0005829cellular_componentcytosol
F0006469biological_processnegative regulation of protein kinase activity
F0006611biological_processprotein export from nucleus
F0007165biological_processsignal transduction
F0008104biological_processprotein localization
F0008426molecular_functionprotein kinase C inhibitor activity
F0008630biological_processintrinsic apoptotic signaling pathway in response to DNA damage
F0010482biological_processregulation of epidermal cell division
F0010839biological_processnegative regulation of keratinocyte proliferation
F0019901molecular_functionprotein kinase binding
F0022407biological_processregulation of cell-cell adhesion
F0030216biological_processkeratinocyte differentiation
F0030307biological_processpositive regulation of cell growth
F0031424biological_processkeratinization
F0032880biological_processregulation of protein localization
F0042742biological_processdefense response to bacterium
F0042802molecular_functionidentical protein binding
F0043588biological_processskin development
F0043616biological_processkeratinocyte proliferation
F0045296molecular_functioncadherin binding
F0045606biological_processpositive regulation of epidermal cell differentiation
F0045785biological_processpositive regulation of cell adhesion
F0045824biological_processnegative regulation of innate immune response
F0046827biological_processpositive regulation of protein export from nucleus
F0050815molecular_functionphosphoserine residue binding
F0051219molecular_functionphosphoprotein binding
F0051715biological_processcytolysis in another organism
F0051726biological_processregulation of cell cycle
F0061436biological_processestablishment of skin barrier
F0070062cellular_componentextracellular exosome
F0072089biological_processstem cell proliferation
F0140311molecular_functionprotein sequestering activity
F0141156biological_processcAMP/PKA signal transduction
F0160075biological_processnon-canonical inflammasome complex assembly
F1903077biological_processnegative regulation of protein localization to plasma membrane
F1903829biological_processpositive regulation of protein localization
F2000647biological_processnegative regulation of stem cell proliferation
Functional Information from PROSITE/UniProt
site_idPS00107
Number of Residues21
DetailsPROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. IGSGSFGTVYkGkwhgd.............VAVK
ChainResidueDetails
AILE355-LYS375
BLEU74-LYS97

site_idPS00108
Number of Residues13
DetailsPROTEIN_KINASE_ST Serine/Threonine protein kinases active-site signature. IiHrDMKsnNIFL
ChainResidueDetails
AILE464-LEU476
BILE186-VAL198

site_idPS00796
Number of Residues11
Details1433_1 14-3-3 proteins signature 1. RNLLSVAYKNV
ChainResidueDetails
EARG41-VAL51

site_idPS00797
Number of Residues20
Details1433_2 14-3-3 proteins signature 2. YKDSTLIMQLLRDNLTLWTA
ChainResidueDetails
ETYR213-ALA232

Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues4
DetailsSITE: Interaction with phosphoserine on interacting protein
ChainResidueDetails
EARG56
EARG129
FARG56
FARG129
CTYR92
CTRP126
CASN139
CGLN152

site_idSWS_FT_FI2
Number of Residues2
DetailsMOD_RES: Phosphoserine => ECO:0007744|PubMed:20068231
ChainResidueDetails
ESER5
DASP208
FSER5
BSER150
BLYS192
BASP208
DLEU74
DMET143
DSER150
DLYS192

site_idSWS_FT_FI3
Number of Residues2
DetailsMOD_RES: Phosphoserine => ECO:0007744|PubMed:23186163
ChainResidueDetails
ESER74
FSER74

site_idSWS_FT_FI4
Number of Residues2
DetailsMOD_RES: Phosphoserine => ECO:0007744|PubMed:16964243, ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:18691976, ECO:0007744|PubMed:20068231
ChainResidueDetails
ESER248
FSER248
DGLU144
DSER194

site_idSWS_FT_FI5
Number of Residues2
DetailsSITE: Cleavage; by anthrax lethal factor
ChainResidueDetails
BPRO8
DPRO8

site_idSWS_FT_FI6
Number of Residues2
DetailsMOD_RES: Phosphoserine; by BRAF and RAF1 => ECO:0000269|PubMed:10409742, ECO:0000269|PubMed:20956560, ECO:0000269|PubMed:29433126, ECO:0000269|PubMed:8131746
ChainResidueDetails
BALA218
DALA218

site_idSWS_FT_FI7
Number of Residues2
DetailsMOD_RES: Phosphoserine; by BRAF and RAF1 => ECO:0000269|PubMed:20956560, ECO:0000269|PubMed:29433126, ECO:0000269|PubMed:8131746
ChainResidueDetails
BALA222
DALA222
CASP340
CASP341

site_idSWS_FT_FI8
Number of Residues2
DetailsMOD_RES: Phosphothreonine => ECO:0007744|PubMed:18691976
ChainResidueDetails
BTHR286
DTHR286

site_idSWS_FT_FI9
Number of Residues2
DetailsMOD_RES: Phosphothreonine; by MAPK1 => ECO:0000250|UniProtKB:Q01986
ChainResidueDetails
BTHR292
DTHR292

site_idSWS_FT_FI10
Number of Residues2
DetailsMOD_RES: Phosphoserine; by PAK => ECO:0000269|PubMed:16129686
ChainResidueDetails
BSER298
DSER298

site_idSWS_FT_FI11
Number of Residues2
DetailsMOD_RES: Phosphoserine; by PKC => ECO:0000269|PubMed:8349614
ChainResidueDetails
ASER499
CSER499

site_idSWS_FT_FI12
Number of Residues2
DetailsMOD_RES: Symmetric dimethylarginine; by PRMT5 => ECO:0000269|PubMed:21917714
ChainResidueDetails
AARG563
CARG563

site_idSWS_FT_FI13
Number of Residues2
DetailsMOD_RES: Phosphoserine => ECO:0000269|PubMed:10801873, ECO:0000269|PubMed:16892053, ECO:0000269|PubMed:21917714, ECO:0000269|PubMed:8349614
ChainResidueDetails
ASEP621
CSEP621

site_idSWS_FT_FI14
Number of Residues2
DetailsMOD_RES: Phosphoserine; by MAPK1 => ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:23186163
ChainResidueDetails
ASER642
CSER642

237423

PDB entries from 2025-06-11

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