9ATU

Bifunctional Inhibition of Neutrophil Elastase by Eap4 from S. aureus

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000122	biological_process	negative regulation of transcription by RNA polymerase II
A	0002020	molecular_function	protease binding
A	0002438	biological_process	acute inflammatory response to antigenic stimulus
A	0002523	biological_process	leukocyte migration involved in inflammatory response
A	0002812	biological_process	biosynthetic process of antibacterial peptides active against Gram-negative bacteria
A	0003714	molecular_function	transcription corepressor activity
A	0004175	molecular_function	endopeptidase activity
A	0004252	molecular_function	serine-type endopeptidase activity
A	0005515	molecular_function	protein binding
A	0005576	cellular_component	extracellular region
A	0005615	cellular_component	extracellular space
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006508	biological_process	proteolysis
A	0006874	biological_process	intracellular calcium ion homeostasis
A	0006909	biological_process	phagocytosis
A	0008201	molecular_function	heparin binding
A	0008233	molecular_function	peptidase activity
A	0008236	molecular_function	serine-type peptidase activity
A	0009411	biological_process	response to UV
A	0009986	cellular_component	cell surface
A	0016787	molecular_function	hydrolase activity
A	0017053	cellular_component	transcription repressor complex
A	0019955	molecular_function	cytokine binding
A	0022617	biological_process	extracellular matrix disassembly
A	0030141	cellular_component	secretory granule
A	0030163	biological_process	protein catabolic process
A	0031012	cellular_component	extracellular matrix
A	0031410	cellular_component	cytoplasmic vesicle
A	0031452	biological_process	negative regulation of heterochromatin formation
A	0032682	biological_process	negative regulation of chemokine production
A	0032717	biological_process	negative regulation of interleukin-8 production
A	0032757	biological_process	positive regulation of interleukin-8 production
A	0035578	cellular_component	azurophil granule lumen
A	0035580	cellular_component	specific granule lumen
A	0042582	cellular_component	azurophil granule
A	0042742	biological_process	defense response to bacterium
A	0043406	biological_process	positive regulation of MAP kinase activity
A	0045335	cellular_component	phagocytic vesicle
A	0048661	biological_process	positive regulation of smooth muscle cell proliferation
A	0050728	biological_process	negative regulation of inflammatory response
A	0050922	biological_process	negative regulation of chemotaxis
A	0070062	cellular_component	extracellular exosome
A	0070269	biological_process	pyroptotic inflammatory response
A	0070944	biological_process	neutrophil-mediated killing of bacterium
A	0070945	biological_process	neutrophil-mediated killing of gram-negative bacterium
A	0070947	biological_process	neutrophil-mediated killing of fungus
A	0140644	cellular_component	neutrophil extracellular trap
A	0140645	biological_process	neutrophil extracellular trap formation
A	1903238	biological_process	positive regulation of leukocyte tethering or rolling
C	0000122	biological_process	negative regulation of transcription by RNA polymerase II
C	0002020	molecular_function	protease binding
C	0002438	biological_process	acute inflammatory response to antigenic stimulus
C	0002523	biological_process	leukocyte migration involved in inflammatory response
C	0002812	biological_process	biosynthetic process of antibacterial peptides active against Gram-negative bacteria
C	0003714	molecular_function	transcription corepressor activity
C	0004175	molecular_function	endopeptidase activity
C	0004252	molecular_function	serine-type endopeptidase activity
C	0005515	molecular_function	protein binding
C	0005576	cellular_component	extracellular region
C	0005615	cellular_component	extracellular space
C	0005634	cellular_component	nucleus
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006508	biological_process	proteolysis
C	0006874	biological_process	intracellular calcium ion homeostasis
C	0006909	biological_process	phagocytosis
C	0008201	molecular_function	heparin binding
C	0008233	molecular_function	peptidase activity
C	0008236	molecular_function	serine-type peptidase activity
C	0009411	biological_process	response to UV
C	0009986	cellular_component	cell surface
C	0016787	molecular_function	hydrolase activity
C	0017053	cellular_component	transcription repressor complex
C	0019955	molecular_function	cytokine binding
C	0022617	biological_process	extracellular matrix disassembly
C	0030141	cellular_component	secretory granule
C	0030163	biological_process	protein catabolic process
C	0031012	cellular_component	extracellular matrix
C	0031410	cellular_component	cytoplasmic vesicle
C	0031452	biological_process	negative regulation of heterochromatin formation
C	0032682	biological_process	negative regulation of chemokine production
C	0032717	biological_process	negative regulation of interleukin-8 production
C	0032757	biological_process	positive regulation of interleukin-8 production
C	0035578	cellular_component	azurophil granule lumen
C	0035580	cellular_component	specific granule lumen
C	0042582	cellular_component	azurophil granule
C	0042742	biological_process	defense response to bacterium
C	0043406	biological_process	positive regulation of MAP kinase activity
C	0045335	cellular_component	phagocytic vesicle
C	0048661	biological_process	positive regulation of smooth muscle cell proliferation
C	0050728	biological_process	negative regulation of inflammatory response
C	0050922	biological_process	negative regulation of chemotaxis
C	0070062	cellular_component	extracellular exosome
C	0070269	biological_process	pyroptotic inflammatory response
C	0070944	biological_process	neutrophil-mediated killing of bacterium
C	0070945	biological_process	neutrophil-mediated killing of gram-negative bacterium
C	0070947	biological_process	neutrophil-mediated killing of fungus
C	0140644	cellular_component	neutrophil extracellular trap
C	0140645	biological_process	neutrophil extracellular trap formation
C	1903238	biological_process	positive regulation of leukocyte tethering or rolling
D	0000122	biological_process	negative regulation of transcription by RNA polymerase II
D	0002020	molecular_function	protease binding
D	0002438	biological_process	acute inflammatory response to antigenic stimulus
D	0002523	biological_process	leukocyte migration involved in inflammatory response
D	0002812	biological_process	biosynthetic process of antibacterial peptides active against Gram-negative bacteria
D	0003714	molecular_function	transcription corepressor activity
D	0004175	molecular_function	endopeptidase activity
D	0004252	molecular_function	serine-type endopeptidase activity
D	0005515	molecular_function	protein binding
D	0005576	cellular_component	extracellular region
D	0005615	cellular_component	extracellular space
D	0005634	cellular_component	nucleus
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006508	biological_process	proteolysis
D	0006874	biological_process	intracellular calcium ion homeostasis
D	0006909	biological_process	phagocytosis
D	0008201	molecular_function	heparin binding
D	0008233	molecular_function	peptidase activity
D	0008236	molecular_function	serine-type peptidase activity
D	0009411	biological_process	response to UV
D	0009986	cellular_component	cell surface
D	0016787	molecular_function	hydrolase activity
D	0017053	cellular_component	transcription repressor complex
D	0019955	molecular_function	cytokine binding
D	0022617	biological_process	extracellular matrix disassembly
D	0030141	cellular_component	secretory granule
D	0030163	biological_process	protein catabolic process
D	0031012	cellular_component	extracellular matrix
D	0031410	cellular_component	cytoplasmic vesicle
D	0031452	biological_process	negative regulation of heterochromatin formation
D	0032682	biological_process	negative regulation of chemokine production
D	0032717	biological_process	negative regulation of interleukin-8 production
D	0032757	biological_process	positive regulation of interleukin-8 production
D	0035578	cellular_component	azurophil granule lumen
D	0035580	cellular_component	specific granule lumen
D	0042582	cellular_component	azurophil granule
D	0042742	biological_process	defense response to bacterium
D	0043406	biological_process	positive regulation of MAP kinase activity
D	0045335	cellular_component	phagocytic vesicle
D	0048661	biological_process	positive regulation of smooth muscle cell proliferation
D	0050728	biological_process	negative regulation of inflammatory response
D	0050922	biological_process	negative regulation of chemotaxis
D	0070062	cellular_component	extracellular exosome
D	0070269	biological_process	pyroptotic inflammatory response
D	0070944	biological_process	neutrophil-mediated killing of bacterium
D	0070945	biological_process	neutrophil-mediated killing of gram-negative bacterium
D	0070947	biological_process	neutrophil-mediated killing of fungus
D	0140644	cellular_component	neutrophil extracellular trap
D	0140645	biological_process	neutrophil extracellular trap formation
D	1903238	biological_process	positive regulation of leukocyte tethering or rolling
F	0000122	biological_process	negative regulation of transcription by RNA polymerase II
F	0002020	molecular_function	protease binding
F	0002438	biological_process	acute inflammatory response to antigenic stimulus
F	0002523	biological_process	leukocyte migration involved in inflammatory response
F	0002812	biological_process	biosynthetic process of antibacterial peptides active against Gram-negative bacteria
F	0003714	molecular_function	transcription corepressor activity
F	0004175	molecular_function	endopeptidase activity
F	0004252	molecular_function	serine-type endopeptidase activity
F	0005515	molecular_function	protein binding
F	0005576	cellular_component	extracellular region
F	0005615	cellular_component	extracellular space
F	0005634	cellular_component	nucleus
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0006508	biological_process	proteolysis
F	0006874	biological_process	intracellular calcium ion homeostasis
F	0006909	biological_process	phagocytosis
F	0008201	molecular_function	heparin binding
F	0008233	molecular_function	peptidase activity
F	0008236	molecular_function	serine-type peptidase activity
F	0009411	biological_process	response to UV
F	0009986	cellular_component	cell surface
F	0016787	molecular_function	hydrolase activity
F	0017053	cellular_component	transcription repressor complex
F	0019955	molecular_function	cytokine binding
F	0022617	biological_process	extracellular matrix disassembly
F	0030141	cellular_component	secretory granule
F	0030163	biological_process	protein catabolic process
F	0031012	cellular_component	extracellular matrix
F	0031410	cellular_component	cytoplasmic vesicle
F	0031452	biological_process	negative regulation of heterochromatin formation
F	0032682	biological_process	negative regulation of chemokine production
F	0032717	biological_process	negative regulation of interleukin-8 production
F	0032757	biological_process	positive regulation of interleukin-8 production
F	0035578	cellular_component	azurophil granule lumen
F	0035580	cellular_component	specific granule lumen
F	0042582	cellular_component	azurophil granule
F	0042742	biological_process	defense response to bacterium
F	0043406	biological_process	positive regulation of MAP kinase activity
F	0045335	cellular_component	phagocytic vesicle
F	0048661	biological_process	positive regulation of smooth muscle cell proliferation
F	0050728	biological_process	negative regulation of inflammatory response
F	0050922	biological_process	negative regulation of chemotaxis
F	0070062	cellular_component	extracellular exosome
F	0070269	biological_process	pyroptotic inflammatory response
F	0070944	biological_process	neutrophil-mediated killing of bacterium
F	0070945	biological_process	neutrophil-mediated killing of gram-negative bacterium
F	0070947	biological_process	neutrophil-mediated killing of fungus
F	0140644	cellular_component	neutrophil extracellular trap
F	0140645	biological_process	neutrophil extracellular trap formation
F	1903238	biological_process	positive regulation of leukocyte tethering or rolling

Functional Information from PROSITE/UniProt

site_id	PS00134
Number of Residues	6
Details	TRYPSIN_HIS Serine proteases, trypsin family, histidine active site. MSAAHC

Chain	Residue	Details
A	MET65-CYS70

---

site_id	PS00135
Number of Residues	12
Details	TRYPSIN_SER Serine proteases, trypsin family, serine active site. GVcfGDSGSPLV

Chain	Residue	Details
A	GLY195-VAL206

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	868
Details	Domain: {"description":"Peptidase S1","evidences":[{"source":"PROSITE-ProRule","id":"PRU00274","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	12
Details	Active site: {"description":"Charge relay system","evidences":[{"source":"PubMed","id":"2911584","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"source":"PubMed","id":"19159218","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"source":"PubMed","id":"26274980","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"3550808","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	4
Details	Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"source":"PubMed","id":"19159218","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"3550808","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---