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8W2H

Human liver phosphofructokinase-1 in the T-state conformation

Functional Information from GO Data
ChainGOidnamespacecontents
A0000166molecular_functionnucleotide binding
A0003824molecular_functioncatalytic activity
A0003872molecular_function6-phosphofructokinase activity
A0005515molecular_functionprotein binding
A0005524molecular_functionATP binding
A0005576cellular_componentextracellular region
A0005737cellular_componentcytoplasm
A0005829cellular_componentcytosol
A0005945cellular_component6-phosphofructokinase complex
A0006002biological_processfructose 6-phosphate metabolic process
A0006096biological_processglycolytic process
A0009749biological_processresponse to glucose
A0016020cellular_componentmembrane
A0016301molecular_functionkinase activity
A0016740molecular_functiontransferase activity
A0019900molecular_functionkinase binding
A0030388biological_processfructose 1,6-bisphosphate metabolic process
A0034774cellular_componentsecretory granule lumen
A0042802molecular_functionidentical protein binding
A0046676biological_processnegative regulation of insulin secretion
A0046872molecular_functionmetal ion binding
A0048029molecular_functionmonosaccharide binding
A0061615biological_processglycolytic process through fructose-6-phosphate
A0061621biological_processcanonical glycolysis
A0070061molecular_functionfructose binding
A0070062cellular_componentextracellular exosome
A0070095molecular_functionfructose-6-phosphate binding
A1904813cellular_componentficolin-1-rich granule lumen
B0000166molecular_functionnucleotide binding
B0003824molecular_functioncatalytic activity
B0003872molecular_function6-phosphofructokinase activity
B0005515molecular_functionprotein binding
B0005524molecular_functionATP binding
B0005576cellular_componentextracellular region
B0005737cellular_componentcytoplasm
B0005829cellular_componentcytosol
B0005945cellular_component6-phosphofructokinase complex
B0006002biological_processfructose 6-phosphate metabolic process
B0006096biological_processglycolytic process
B0009749biological_processresponse to glucose
B0016020cellular_componentmembrane
B0016301molecular_functionkinase activity
B0016740molecular_functiontransferase activity
B0019900molecular_functionkinase binding
B0030388biological_processfructose 1,6-bisphosphate metabolic process
B0034774cellular_componentsecretory granule lumen
B0042802molecular_functionidentical protein binding
B0046676biological_processnegative regulation of insulin secretion
B0046872molecular_functionmetal ion binding
B0048029molecular_functionmonosaccharide binding
B0061615biological_processglycolytic process through fructose-6-phosphate
B0061621biological_processcanonical glycolysis
B0070061molecular_functionfructose binding
B0070062cellular_componentextracellular exosome
B0070095molecular_functionfructose-6-phosphate binding
B1904813cellular_componentficolin-1-rich granule lumen
C0000166molecular_functionnucleotide binding
C0003824molecular_functioncatalytic activity
C0003872molecular_function6-phosphofructokinase activity
C0005515molecular_functionprotein binding
C0005524molecular_functionATP binding
C0005576cellular_componentextracellular region
C0005737cellular_componentcytoplasm
C0005829cellular_componentcytosol
C0005945cellular_component6-phosphofructokinase complex
C0006002biological_processfructose 6-phosphate metabolic process
C0006096biological_processglycolytic process
C0009749biological_processresponse to glucose
C0016020cellular_componentmembrane
C0016301molecular_functionkinase activity
C0016740molecular_functiontransferase activity
C0019900molecular_functionkinase binding
C0030388biological_processfructose 1,6-bisphosphate metabolic process
C0034774cellular_componentsecretory granule lumen
C0042802molecular_functionidentical protein binding
C0046676biological_processnegative regulation of insulin secretion
C0046872molecular_functionmetal ion binding
C0048029molecular_functionmonosaccharide binding
C0061615biological_processglycolytic process through fructose-6-phosphate
C0061621biological_processcanonical glycolysis
C0070061molecular_functionfructose binding
C0070062cellular_componentextracellular exosome
C0070095molecular_functionfructose-6-phosphate binding
C1904813cellular_componentficolin-1-rich granule lumen
D0000166molecular_functionnucleotide binding
D0003824molecular_functioncatalytic activity
D0003872molecular_function6-phosphofructokinase activity
D0005515molecular_functionprotein binding
D0005524molecular_functionATP binding
D0005576cellular_componentextracellular region
D0005737cellular_componentcytoplasm
D0005829cellular_componentcytosol
D0005945cellular_component6-phosphofructokinase complex
D0006002biological_processfructose 6-phosphate metabolic process
D0006096biological_processglycolytic process
D0009749biological_processresponse to glucose
D0016020cellular_componentmembrane
D0016301molecular_functionkinase activity
D0016740molecular_functiontransferase activity
D0019900molecular_functionkinase binding
D0030388biological_processfructose 1,6-bisphosphate metabolic process
D0034774cellular_componentsecretory granule lumen
D0042802molecular_functionidentical protein binding
D0046676biological_processnegative regulation of insulin secretion
D0046872molecular_functionmetal ion binding
D0048029molecular_functionmonosaccharide binding
D0061615biological_processglycolytic process through fructose-6-phosphate
D0061621biological_processcanonical glycolysis
D0070061molecular_functionfructose binding
D0070062cellular_componentextracellular exosome
D0070095molecular_functionfructose-6-phosphate binding
D1904813cellular_componentficolin-1-rich granule lumen
Functional Information from PROSITE/UniProt
site_idPS00433
Number of Residues19
DetailsPHOSPHOFRUCTOKINASE Phosphofructokinase signature. RvtvlGHvQRGGtpsafDR
ChainResidueDetails
AARG292-ARG310
AARG654-ARG672

Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues4
DetailsACT_SITE: Proton acceptor => ECO:0000255|HAMAP-Rule:MF_03184
ChainResidueDetails
AASP166
BASP166
CASP166
DASP166

site_idSWS_FT_FI2
Number of Residues32
DetailsBINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_03184
ChainResidueDetails
AGLY25
BARG88
BGLY118
BASP119
BARG201
BARG292
BARG565
BARG654
CGLY25
CARG88
CGLY118
AARG88
CASP119
CARG201
CARG292
CARG565
CARG654
DGLY25
DARG88
DGLY118
DASP119
DARG201
AGLY118
DARG292
DARG565
DARG654
AASP119
AARG201
AARG292
AARG565
AARG654
BGLY25

site_idSWS_FT_FI3
Number of Residues40
DetailsBINDING: in other chain => ECO:0000255|HAMAP-Rule:MF_03184
ChainResidueDetails
ASER164
AARG734
BSER164
BMET208
BGLU264
BHIS298
BARG470
BTHR527
BMET572
BGLU628
BHIS660
AMET208
BARG734
CSER164
CMET208
CGLU264
CHIS298
CARG470
CTHR527
CMET572
CGLU628
CHIS660
AGLU264
CARG734
DSER164
DMET208
DGLU264
DHIS298
DARG470
DTHR527
DMET572
DGLU628
DHIS660
AHIS298
DARG734
AARG470
ATHR527
AMET572
AGLU628
AHIS660

site_idSWS_FT_FI4
Number of Residues4
DetailsMOD_RES: N-acetylalanine => ECO:0000269|Ref.6, ECO:0007744|PubMed:22223895, ECO:0007744|PubMed:22814378
ChainResidueDetails
AALA2
BALA2
CALA2
DALA2

site_idSWS_FT_FI5
Number of Residues4
DetailsMOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P47857
ChainResidueDetails
ASER377
BSER377
CSER377
DSER377

site_idSWS_FT_FI6
Number of Residues4
DetailsMOD_RES: Phosphotyrosine => ECO:0000250|UniProtKB:P12382
ChainResidueDetails
ATYR640
BTYR640
CTYR640
DTYR640

site_idSWS_FT_FI7
Number of Residues4
DetailsMOD_RES: Phosphoserine => ECO:0007744|PubMed:18669648
ChainResidueDetails
ASER775
BSER775
CSER775
DSER775

site_idSWS_FT_FI8
Number of Residues4
DetailsCARBOHYD: O-linked (GlcNAc) serine => ECO:0000269|PubMed:22923583
ChainResidueDetails
ASER529
BSER529
CSER529
DSER529

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PDB entries from 2025-06-18

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