8UW2

Structure of AKT1(E17K) with compound 3 (zinc-free)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0001649	biological_process	osteoblast differentiation
A	0001893	biological_process	maternal placenta development
A	0001938	biological_process	positive regulation of endothelial cell proliferation
A	0002042	biological_process	cell migration involved in sprouting angiogenesis
A	0002181	biological_process	cytoplasmic translation
A	0002430	biological_process	complement receptor mediated signaling pathway
A	0003376	biological_process	sphingosine-1-phosphate receptor signaling pathway
A	0004672	molecular_function	protein kinase activity
A	0004674	molecular_function	protein serine/threonine kinase activity
A	0004712	molecular_function	protein serine/threonine/tyrosine kinase activity
A	0005515	molecular_function	protein binding
A	0005516	molecular_function	calmodulin binding
A	0005524	molecular_function	ATP binding
A	0005547	molecular_function	phosphatidylinositol-3,4,5-trisphosphate binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005758	cellular_component	mitochondrial intermembrane space
A	0005783	cellular_component	endoplasmic reticulum
A	0005819	cellular_component	spindle
A	0005829	cellular_component	cytosol
A	0005886	cellular_component	plasma membrane
A	0005911	cellular_component	cell-cell junction
A	0005929	cellular_component	cilium
A	0005938	cellular_component	cell cortex
A	0005977	biological_process	glycogen metabolic process
A	0005978	biological_process	glycogen biosynthetic process
A	0005979	biological_process	regulation of glycogen biosynthetic process
A	0006006	biological_process	glucose metabolic process
A	0006094	biological_process	gluconeogenesis
A	0006413	biological_process	translational initiation
A	0006417	biological_process	regulation of translation
A	0006468	biological_process	protein phosphorylation
A	0006511	biological_process	ubiquitin-dependent protein catabolic process
A	0006606	biological_process	protein import into nucleus
A	0006607	biological_process	NLS-bearing protein import into nucleus
A	0006809	biological_process	nitric oxide biosynthetic process
A	0006888	biological_process	endoplasmic reticulum to Golgi vesicle-mediated transport
A	0006893	biological_process	Golgi to plasma membrane transport
A	0006915	biological_process	apoptotic process
A	0006924	biological_process	activation-induced cell death of T cells
A	0006954	biological_process	inflammatory response
A	0006979	biological_process	response to oxidative stress
A	0007165	biological_process	signal transduction
A	0007173	biological_process	epidermal growth factor receptor signaling pathway
A	0007186	biological_process	G protein-coupled receptor signaling pathway
A	0007281	biological_process	germ cell development
A	0007399	biological_process	nervous system development
A	0008283	biological_process	cell population proliferation
A	0008286	biological_process	insulin receptor signaling pathway
A	0008637	biological_process	apoptotic mitochondrial changes
A	0009267	biological_process	cellular response to starvation
A	0009408	biological_process	response to heat
A	0009725	biological_process	response to hormone
A	0010467	biological_process	gene expression
A	0010507	biological_process	negative regulation of autophagy
A	0010595	biological_process	positive regulation of endothelial cell migration
A	0010628	biological_process	positive regulation of gene expression
A	0010629	biological_process	negative regulation of gene expression
A	0010748	biological_process	negative regulation of long-chain fatty acid import across plasma membrane
A	0010761	biological_process	fibroblast migration
A	0010763	biological_process	positive regulation of fibroblast migration
A	0010765	biological_process	positive regulation of sodium ion transport
A	0010907	biological_process	positive regulation of glucose metabolic process
A	0010975	biological_process	regulation of neuron projection development
A	0015630	cellular_component	microtubule cytoskeleton
A	0016020	cellular_component	membrane
A	0016242	biological_process	negative regulation of macroautophagy
A	0016301	molecular_function	kinase activity
A	0016310	biological_process	phosphorylation
A	0016477	biological_process	cell migration
A	0016567	biological_process	protein ubiquitination
A	0016740	molecular_function	transferase activity
A	0018105	biological_process	peptidyl-serine phosphorylation
A	0018107	biological_process	peptidyl-threonine phosphorylation
A	0019221	biological_process	cytokine-mediated signaling pathway
A	0019899	molecular_function	enzyme binding
A	0019900	molecular_function	kinase binding
A	0019901	molecular_function	protein kinase binding
A	0022605	biological_process	mammalian oogenesis stage
A	0030027	cellular_component	lamellipodium
A	0030154	biological_process	cell differentiation
A	0030163	biological_process	protein catabolic process
A	0030235	molecular_function	nitric-oxide synthase regulator activity
A	0030291	molecular_function	protein serine/threonine kinase inhibitor activity
A	0030307	biological_process	positive regulation of cell growth
A	0030334	biological_process	regulation of cell migration
A	0030335	biological_process	positive regulation of cell migration
A	0030336	biological_process	negative regulation of cell migration
A	0031145	biological_process	anaphase-promoting complex-dependent catabolic process
A	0031146	biological_process	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
A	0031295	biological_process	T cell costimulation
A	0031397	biological_process	negative regulation of protein ubiquitination
A	0031641	biological_process	regulation of myelination
A	0031663	biological_process	lipopolysaccharide-mediated signaling pathway
A	0031669	biological_process	cellular response to nutrient levels
A	0031929	biological_process	TOR signaling
A	0031982	cellular_component	vesicle
A	0031999	biological_process	negative regulation of fatty acid beta-oxidation
A	0032079	biological_process	positive regulation of endodeoxyribonuclease activity
A	0032094	biological_process	response to food
A	0032287	biological_process	peripheral nervous system myelin maintenance
A	0032436	biological_process	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
A	0032869	biological_process	cellular response to insulin stimulus
A	0032880	biological_process	regulation of protein localization
A	0032991	cellular_component	protein-containing complex
A	0033138	biological_process	positive regulation of peptidyl-serine phosphorylation
A	0033554	biological_process	cellular response to stress
A	0034122	biological_process	negative regulation of toll-like receptor signaling pathway
A	0034123	biological_process	positive regulation of toll-like receptor signaling pathway
A	0034142	biological_process	toll-like receptor 4 signaling pathway
A	0034198	biological_process	cellular response to amino acid starvation
A	0034405	biological_process	response to fluid shear stress
A	0035519	biological_process	protein K29-linked ubiquitination
A	0035556	biological_process	intracellular signal transduction
A	0035655	biological_process	interleukin-18-mediated signaling pathway
A	0035924	biological_process	cellular response to vascular endothelial growth factor stimulus
A	0036064	cellular_component	ciliary basal body
A	0036294	biological_process	cellular response to decreased oxygen levels
A	0036316	biological_process	SREBP-SCAP complex retention in endoplasmic reticulum
A	0036416	biological_process	tRNA stabilization
A	0036444	biological_process	calcium import into the mitochondrion
A	0036499	biological_process	PERK-mediated unfolded protein response
A	0038202	biological_process	TORC1 signaling
A	0042593	biological_process	glucose homeostasis
A	0042802	molecular_function	identical protein binding
A	0042803	molecular_function	protein homodimerization activity
A	0042981	biological_process	regulation of apoptotic process
A	0043001	biological_process	Golgi to plasma membrane protein transport
A	0043066	biological_process	negative regulation of apoptotic process
A	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
A	0043276	biological_process	anoikis
A	0043325	molecular_function	phosphatidylinositol-3,4-bisphosphate binding
A	0043488	biological_process	regulation of mRNA stability
A	0043491	biological_process	phosphatidylinositol 3-kinase/protein kinase B signal transduction
A	0043536	biological_process	positive regulation of blood vessel endothelial cell migration
A	0045429	biological_process	positive regulation of nitric oxide biosynthetic process
A	0045542	biological_process	positive regulation of cholesterol biosynthetic process
A	0045600	biological_process	positive regulation of fat cell differentiation
A	0045724	biological_process	positive regulation of cilium assembly
A	0045725	biological_process	positive regulation of glycogen biosynthetic process
A	0045746	biological_process	negative regulation of Notch signaling pathway
A	0045861	biological_process	negative regulation of proteolysis
A	0045944	biological_process	positive regulation of transcription by RNA polymerase II
A	0045947	biological_process	negative regulation of translational initiation
A	0045948	biological_process	positive regulation of translational initiation
A	0046209	biological_process	nitric oxide metabolic process
A	0046326	biological_process	positive regulation of D-glucose import
A	0046622	biological_process	positive regulation of organ growth
A	0046777	biological_process	protein autophosphorylation
A	0046889	biological_process	positive regulation of lipid biosynthetic process
A	0048009	biological_process	insulin-like growth factor receptor signaling pathway
A	0048266	biological_process	behavioral response to pain
A	0048661	biological_process	positive regulation of smooth muscle cell proliferation
A	0051146	biological_process	striated muscle cell differentiation
A	0051247	biological_process	positive regulation of protein metabolic process
A	0051604	biological_process	protein maturation
A	0060079	biological_process	excitatory postsynaptic potential
A	0060416	biological_process	response to growth hormone
A	0060627	biological_process	regulation of vesicle-mediated transport
A	0060644	biological_process	mammary gland epithelial cell differentiation
A	0060716	biological_process	labyrinthine layer blood vessel development
A	0061512	biological_process	protein localization to cilium
A	0070141	biological_process	response to UV-A
A	0070848	biological_process	response to growth factor
A	0070972	biological_process	protein localization to endoplasmic reticulum
A	0070979	biological_process	protein K11-linked ubiquitination
A	0071277	biological_process	cellular response to calcium ion
A	0071356	biological_process	cellular response to tumor necrosis factor
A	0071363	biological_process	cellular response to growth factor stimulus
A	0071364	biological_process	cellular response to epidermal growth factor stimulus
A	0071380	biological_process	cellular response to prostaglandin E stimulus
A	0071889	molecular_function	14-3-3 protein binding
A	0072350	biological_process	tricarboxylic acid metabolic process
A	0072655	biological_process	establishment of protein localization to mitochondrion
A	0072656	biological_process	maintenance of protein location in mitochondrion
A	0072752	biological_process	cellular response to rapamycin
A	0085020	biological_process	protein K6-linked ubiquitination
A	0090201	biological_process	negative regulation of release of cytochrome c from mitochondria
A	0097011	biological_process	cellular response to granulocyte macrophage colony-stimulating factor stimulus
A	0097194	biological_process	execution phase of apoptosis
A	0097700	biological_process	vascular endothelial cell response to laminar fluid shear stress
A	0098794	cellular_component	postsynapse
A	0098978	cellular_component	glutamatergic synapse
A	0099104	molecular_function	potassium channel activator activity
A	0099175	biological_process	regulation of postsynapse organization
A	0106004	biological_process	tRNA (guanine-N7)-methylation
A	0106310	molecular_function	protein serine kinase activity
A	0110002	biological_process	regulation of tRNA methylation
A	0140052	biological_process	cellular response to oxidised low-density lipoprotein particle stimulus
A	0140896	biological_process	cGAS/STING signaling pathway
A	0150033	biological_process	negative regulation of protein localization to lysosome
A	0160049	biological_process	negative regulation of cGAS/STING signaling pathway
A	0160213	biological_process	beta-arrestin-dependent dopamine receptor signaling pathway
A	1900087	biological_process	positive regulation of G1/S transition of mitotic cell cycle
A	1900182	biological_process	positive regulation of protein localization to nucleus
A	1901653	biological_process	cellular response to peptide
A	1901796	biological_process	regulation of signal transduction by p53 class mediator
A	1902018	biological_process	negative regulation of cilium assembly
A	1902176	biological_process	negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
A	1903038	biological_process	negative regulation of leukocyte cell-cell adhesion
A	1903078	biological_process	positive regulation of protein localization to plasma membrane
A	1903318	biological_process	negative regulation of protein maturation
A	1903384	biological_process	negative regulation of hydrogen peroxide-induced neuron intrinsic apoptotic signaling pathway
A	1903898	biological_process	negative regulation of PERK-mediated unfolded protein response
A	1904262	biological_process	negative regulation of TORC1 signaling
A	1904263	biological_process	positive regulation of TORC1 signaling
A	1904515	biological_process	positive regulation of TORC2 signaling
A	1904841	molecular_function	TORC2 complex binding
A	1905552	biological_process	positive regulation of protein localization to endoplasmic reticulum
A	1905786	biological_process	positive regulation of anaphase-promoting complex-dependent catabolic process
A	1990090	biological_process	cellular response to nerve growth factor stimulus
A	1990418	biological_process	response to insulin-like growth factor stimulus
A	2000010	biological_process	positive regulation of protein localization to cell surface
A	2000074	biological_process	regulation of type B pancreatic cell development
A	2000402	biological_process	negative regulation of lymphocyte migration
A	2001240	biological_process	negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
A	2001243	biological_process	negative regulation of intrinsic apoptotic signaling pathway

Functional Information from PROSITE/UniProt

site_id	PS00107
Number of Residues	34
Details	PROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. LGKGTFGKVIlVkekatgryyamkilkkevIVAK

Chain	Residue	Details
A	LEU156-LYS189

---

site_id	PS00108
Number of Residues	13
Details	PROTEIN_KINASE_ST Serine/Threonine protein kinases active-site signature. VvYrDLKleNLML

Chain	Residue	Details
A	VAL270-LEU282

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"PROSITE-ProRule","id":"PRU00159","evidenceCode":"ECO:0000255"},{"source":"PROSITE-ProRule","id":"PRU10027","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	9
Details	Binding site: {"evidences":[{"source":"PubMed","id":"12176338","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"12964941","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	9
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00159","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"21775285","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	1
Details	Modified residue: {"description":"Phosphoserine; alternate","evidences":[{"source":"PubMed","id":"17081983","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"18669648","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	1
Details	Modified residue: {"description":"Phosphotyrosine; by TNK2","evidences":[{"source":"PubMed","id":"20333297","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	1
Details	Modified residue: {"description":"Phosphothreonine; by IKKE, PDPK1 and TBK1","evidences":[{"source":"PubMed","id":"15718470","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"18456494","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"20333297","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"20481595","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"21464307","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8978681","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9512493","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	1
Details	Glycosylation: {"description":"O-linked (GlcNAc) serine; alternate","evidences":[{"source":"PubMed","id":"22629392","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	1
Details	Glycosylation: {"description":"O-linked (GlcNAc) threonine","evidences":[{"source":"PubMed","id":"22629392","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	2
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"PubMed","id":"22410793","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---