Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

8UII

Cryo-EM map of human clamp-clamp loader ATAD5-RFC-closed PCNA complex in intermediate state 1

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0003677	molecular_function	DNA binding
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005634	cellular_component	nucleus
A	0006974	biological_process	DNA damage response
A	0008283	biological_process	cell population proliferation
A	0016887	molecular_function	ATP hydrolysis activity
A	0031391	cellular_component	Elg1 RFC-like complex
A	0033260	biological_process	nuclear DNA replication
A	0045740	biological_process	positive regulation of DNA replication
A	0051054	biological_process	positive regulation of DNA metabolic process
A	0061860	molecular_function	DNA clamp unloader activity
A	1901987	biological_process	regulation of cell cycle phase transition
A	1902166	biological_process	negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
A	1902751	biological_process	positive regulation of cell cycle G2/M phase transition
B	0000076	biological_process	DNA replication checkpoint signaling
B	0000166	molecular_function	nucleotide binding
B	0003677	molecular_function	DNA binding
B	0003689	molecular_function	DNA clamp loader activity
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005663	cellular_component	DNA replication factor C complex
B	0005694	cellular_component	chromosome
B	0006260	biological_process	DNA replication
B	0006261	biological_process	DNA-templated DNA replication
B	0006281	biological_process	DNA repair
B	0016887	molecular_function	ATP hydrolysis activity
B	0017116	molecular_function	single-stranded DNA helicase activity
B	0019899	molecular_function	enzyme binding
B	0031390	cellular_component	Ctf18 RFC-like complex
B	1900264	biological_process	positive regulation of DNA-directed DNA polymerase activity
C	0000076	biological_process	DNA replication checkpoint signaling
C	0000166	molecular_function	nucleotide binding
C	0003677	molecular_function	DNA binding
C	0003689	molecular_function	DNA clamp loader activity
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005663	cellular_component	DNA replication factor C complex
C	0005694	cellular_component	chromosome
C	0006260	biological_process	DNA replication
C	0006261	biological_process	DNA-templated DNA replication
C	0006281	biological_process	DNA repair
C	0016887	molecular_function	ATP hydrolysis activity
C	0017116	molecular_function	single-stranded DNA helicase activity
C	0019899	molecular_function	enzyme binding
C	0031390	cellular_component	Ctf18 RFC-like complex
C	1900264	biological_process	positive regulation of DNA-directed DNA polymerase activity
D	0000076	biological_process	DNA replication checkpoint signaling
D	0000166	molecular_function	nucleotide binding
D	0003677	molecular_function	DNA binding
D	0003689	molecular_function	DNA clamp loader activity
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005663	cellular_component	DNA replication factor C complex
D	0005694	cellular_component	chromosome
D	0006260	biological_process	DNA replication
D	0006261	biological_process	DNA-templated DNA replication
D	0006271	biological_process	DNA strand elongation involved in DNA replication
D	0006281	biological_process	DNA repair
D	0016887	molecular_function	ATP hydrolysis activity
D	0017116	molecular_function	single-stranded DNA helicase activity
D	0019899	molecular_function	enzyme binding
D	0031390	cellular_component	Ctf18 RFC-like complex
D	0031391	cellular_component	Elg1 RFC-like complex
D	1900264	biological_process	positive regulation of DNA-directed DNA polymerase activity
E	0000076	biological_process	DNA replication checkpoint signaling
E	0000731	biological_process	DNA synthesis involved in DNA repair
E	0003677	molecular_function	DNA binding
E	0003689	molecular_function	DNA clamp loader activity
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005654	cellular_component	nucleoplasm
E	0005663	cellular_component	DNA replication factor C complex
E	0005694	cellular_component	chromosome
E	0006260	biological_process	DNA replication
E	0006261	biological_process	DNA-templated DNA replication
E	0006271	biological_process	DNA strand elongation involved in DNA replication
E	0006281	biological_process	DNA repair
E	0008094	molecular_function	ATP-dependent activity, acting on DNA
E	0017116	molecular_function	single-stranded DNA helicase activity
E	0031390	cellular_component	Ctf18 RFC-like complex
E	0032991	cellular_component	protein-containing complex
E	1900264	biological_process	positive regulation of DNA-directed DNA polymerase activity
F	0000307	cellular_component	cyclin-dependent protein kinase holoenzyme complex
F	0000701	molecular_function	purine-specific mismatch base pair DNA N-glycosylase activity
F	0000781	cellular_component	chromosome, telomeric region
F	0000785	cellular_component	chromatin
F	0003677	molecular_function	DNA binding
F	0003682	molecular_function	chromatin binding
F	0003684	molecular_function	damaged DNA binding
F	0005515	molecular_function	protein binding
F	0005634	cellular_component	nucleus
F	0005652	cellular_component	nuclear lamina
F	0005654	cellular_component	nucleoplasm
F	0005657	cellular_component	replication fork
F	0005813	cellular_component	centrosome
F	0006260	biological_process	DNA replication
F	0006272	biological_process	leading strand elongation
F	0006275	biological_process	regulation of DNA replication
F	0006281	biological_process	DNA repair
F	0006298	biological_process	mismatch repair
F	0006325	biological_process	chromatin organization
F	0006974	biological_process	DNA damage response
F	0006979	biological_process	response to oxidative stress
F	0007507	biological_process	heart development
F	0019899	molecular_function	enzyme binding
F	0019985	biological_process	translesion synthesis
F	0030331	molecular_function	nuclear estrogen receptor binding
F	0030337	molecular_function	DNA polymerase processivity factor activity
F	0030855	biological_process	epithelial cell differentiation
F	0030894	cellular_component	replisome
F	0030971	molecular_function	receptor tyrosine kinase binding
F	0031297	biological_process	replication fork processing
F	0032139	molecular_function	dinucleotide insertion or deletion binding
F	0032355	biological_process	response to estradiol
F	0032405	molecular_function	MutLalpha complex binding
F	0033993	biological_process	response to lipid
F	0034644	biological_process	cellular response to UV
F	0035035	molecular_function	histone acetyltransferase binding
F	0042802	molecular_function	identical protein binding
F	0043596	cellular_component	nuclear replication fork
F	0043626	cellular_component	PCNA complex
F	0044849	biological_process	estrous cycle
F	0044877	molecular_function	protein-containing complex binding
F	0045739	biological_process	positive regulation of DNA repair
F	0045740	biological_process	positive regulation of DNA replication
F	0046686	biological_process	response to cadmium ion
F	0070062	cellular_component	extracellular exosome
F	0070182	molecular_function	DNA polymerase binding
F	0070301	biological_process	cellular response to hydrogen peroxide
F	0070557	cellular_component	PCNA-p21 complex
F	0071548	biological_process	response to dexamethasone
F	0097421	biological_process	liver regeneration
F	0140861	biological_process	DNA repair-dependent chromatin remodeling
F	1902065	biological_process	response to L-glutamate
F	1902969	biological_process	mitotic DNA replication
F	1902990	biological_process	mitotic telomere maintenance via semi-conservative replication
G	0000307	cellular_component	cyclin-dependent protein kinase holoenzyme complex
G	0000701	molecular_function	purine-specific mismatch base pair DNA N-glycosylase activity
G	0000781	cellular_component	chromosome, telomeric region
G	0000785	cellular_component	chromatin
G	0003677	molecular_function	DNA binding
G	0003682	molecular_function	chromatin binding
G	0003684	molecular_function	damaged DNA binding
G	0005515	molecular_function	protein binding
G	0005634	cellular_component	nucleus
G	0005652	cellular_component	nuclear lamina
G	0005654	cellular_component	nucleoplasm
G	0005657	cellular_component	replication fork
G	0005813	cellular_component	centrosome
G	0006260	biological_process	DNA replication
G	0006272	biological_process	leading strand elongation
G	0006275	biological_process	regulation of DNA replication
G	0006281	biological_process	DNA repair
G	0006298	biological_process	mismatch repair
G	0006325	biological_process	chromatin organization
G	0006974	biological_process	DNA damage response
G	0006979	biological_process	response to oxidative stress
G	0007507	biological_process	heart development
G	0019899	molecular_function	enzyme binding
G	0019985	biological_process	translesion synthesis
G	0030331	molecular_function	nuclear estrogen receptor binding
G	0030337	molecular_function	DNA polymerase processivity factor activity
G	0030855	biological_process	epithelial cell differentiation
G	0030894	cellular_component	replisome
G	0030971	molecular_function	receptor tyrosine kinase binding
G	0031297	biological_process	replication fork processing
G	0032139	molecular_function	dinucleotide insertion or deletion binding
G	0032355	biological_process	response to estradiol
G	0032405	molecular_function	MutLalpha complex binding
G	0033993	biological_process	response to lipid
G	0034644	biological_process	cellular response to UV
G	0035035	molecular_function	histone acetyltransferase binding
G	0042802	molecular_function	identical protein binding
G	0043596	cellular_component	nuclear replication fork
G	0043626	cellular_component	PCNA complex
G	0044849	biological_process	estrous cycle
G	0044877	molecular_function	protein-containing complex binding
G	0045739	biological_process	positive regulation of DNA repair
G	0045740	biological_process	positive regulation of DNA replication
G	0046686	biological_process	response to cadmium ion
G	0070062	cellular_component	extracellular exosome
G	0070182	molecular_function	DNA polymerase binding
G	0070301	biological_process	cellular response to hydrogen peroxide
G	0070557	cellular_component	PCNA-p21 complex
G	0071548	biological_process	response to dexamethasone
G	0097421	biological_process	liver regeneration
G	0140861	biological_process	DNA repair-dependent chromatin remodeling
G	1902065	biological_process	response to L-glutamate
G	1902969	biological_process	mitotic DNA replication
G	1902990	biological_process	mitotic telomere maintenance via semi-conservative replication
H	0000307	cellular_component	cyclin-dependent protein kinase holoenzyme complex
H	0000701	molecular_function	purine-specific mismatch base pair DNA N-glycosylase activity
H	0000781	cellular_component	chromosome, telomeric region
H	0000785	cellular_component	chromatin
H	0003677	molecular_function	DNA binding
H	0003682	molecular_function	chromatin binding
H	0003684	molecular_function	damaged DNA binding
H	0005515	molecular_function	protein binding
H	0005634	cellular_component	nucleus
H	0005652	cellular_component	nuclear lamina
H	0005654	cellular_component	nucleoplasm
H	0005657	cellular_component	replication fork
H	0005813	cellular_component	centrosome
H	0006260	biological_process	DNA replication
H	0006272	biological_process	leading strand elongation
H	0006275	biological_process	regulation of DNA replication
H	0006281	biological_process	DNA repair
H	0006298	biological_process	mismatch repair
H	0006325	biological_process	chromatin organization
H	0006974	biological_process	DNA damage response
H	0006979	biological_process	response to oxidative stress
H	0007507	biological_process	heart development
H	0019899	molecular_function	enzyme binding
H	0019985	biological_process	translesion synthesis
H	0030331	molecular_function	nuclear estrogen receptor binding
H	0030337	molecular_function	DNA polymerase processivity factor activity
H	0030855	biological_process	epithelial cell differentiation
H	0030894	cellular_component	replisome
H	0030971	molecular_function	receptor tyrosine kinase binding
H	0031297	biological_process	replication fork processing
H	0032139	molecular_function	dinucleotide insertion or deletion binding
H	0032355	biological_process	response to estradiol
H	0032405	molecular_function	MutLalpha complex binding
H	0033993	biological_process	response to lipid
H	0034644	biological_process	cellular response to UV
H	0035035	molecular_function	histone acetyltransferase binding
H	0042802	molecular_function	identical protein binding
H	0043596	cellular_component	nuclear replication fork
H	0043626	cellular_component	PCNA complex
H	0044849	biological_process	estrous cycle
H	0044877	molecular_function	protein-containing complex binding
H	0045739	biological_process	positive regulation of DNA repair
H	0045740	biological_process	positive regulation of DNA replication
H	0046686	biological_process	response to cadmium ion
H	0070062	cellular_component	extracellular exosome
H	0070182	molecular_function	DNA polymerase binding
H	0070301	biological_process	cellular response to hydrogen peroxide
H	0070557	cellular_component	PCNA-p21 complex
H	0071548	biological_process	response to dexamethasone
H	0097421	biological_process	liver regeneration
H	0140861	biological_process	DNA repair-dependent chromatin remodeling
H	1902065	biological_process	response to L-glutamate
H	1902969	biological_process	mitotic DNA replication
H	1902990	biological_process	mitotic telomere maintenance via semi-conservative replication

Functional Information from PROSITE/UniProt

site_id	PS00293
Number of Residues	19
Details	PCNA_2 Proliferating cell nuclear antigen signature 2. RCDRnlaMgvnLtSMsKIL

Chain	Residue	Details
F	ARG61-LEU79

site_id	PS01251
Number of Residues	24
Details	PCNA_1 Proliferating cell nuclear antigen signature 1. GVnLqSMDsSHVsLVqLtLrsegF

Chain	Residue	Details
F	GLY34-PHE57

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	7
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00499","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	21
Details	Binding site: {"evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	12
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"19608861","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	1
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"17525332","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	57
Details	DNA binding: {"evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	279
Details	Region: {"description":"Interaction with NUDT15","evidences":[{"source":"PubMed","id":"19419956","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI7
Number of Residues	3
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"19419956","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI8
Number of Residues	3
Details	Modified residue: {"description":"Phosphotyrosine; by EGFR","evidences":[{"source":"PubMed","id":"17115032","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"38459011","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI9
Number of Residues	3
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate","evidences":[{"source":"PubMed","id":"17108083","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"17130289","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI10
Number of Residues	6
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)","evidences":[{"source":"PubMed","id":"28112733","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)